Pericytes Suppress Brain Metastasis from Lung Cancer In Vitro
Metastasis of lung cancer to the brain is associated with poor outcomes and limited therapeutic options. The blood–brain barrier (BBB) plays a major role in brain metastasis. However, little is known about the role of pericytes in brain metastasis formation. This study aimed to reveal the interaction between pericytes and cancer cells. We established in vitro BBB models with rat primary cultured BBB-related cells (endothelial cells, astrocytes, and pericytes) and investigated the relationship between BBB-related cells and metastatic cancer cell lines. We observed a significant decrease in transendothelial electrical resistance with metastatic cancer cells in monolayer and coculture models with astrocytes. In contrast, the coculture model with pericytes showed inhibition of the decrease in transendothelial electrical resistance with metastatic cancer cells. In addition, the expression of tight junction protein was preserved only in the coculture model with pericytes. The conditioned medium of pericytes with metastatic cancer cells suppressed the proliferation of the cancer cells significantly. This study revealed that brain pericytes are the major regulators of the resistance of the BBB to lung cancer metastasis to the brain. Pericytes exert an anti-metastatic effect and thus have potential for the preventive treatment of brain metastasis.
KeywordsBlood–brain barrier Pericytes Brain metastasis Lung cancer
We thank Jun Aruga for critical review of the manuscript and helpful professional guidance. We also thank Mitchell Arico from Edanz Group (www.edanzediting.com/ac) for editing a draft of this manuscript.
T.F. and Y.M. designed the study and wrote the initial draft of the manuscript. S.N contributed to analysis and interpretation of data, M.N. and M.D. were involved in planning, and also supervised the work. All other authors have contributed to data collection and interpretation, and critically reviewed the manuscript. All authors approved the final version of the manuscript and agree to be accountable for all aspects of the work in ensuring that questions related to the accuracy or integrity of any part of the work are appropriately investigated and resolved.
This study was funded by JSPS and HAS under the Japan-Hungary Research Cooperative Program (to Y.M. and M.D.) and, in part, by Grants-in-Aid for Scientific Research (C) 17K10840 (to Y.M.), (C) 15KK0349 (to Y.M.), and (C) 17K10839 (to K.U.).
Compliance with Ethical Standards
Conflict of interest
All authors have no conflicts of interest to disclose.
All applicable international, national, and/or institutional guidelines for the care and use of animals were followed.
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