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Cellular and Molecular Neurobiology

, Volume 39, Issue 7, pp 975–983 | Cite as

CA1 LTP Attenuated by Corticosterone is Canceled by Effusol via Rescuing Intracellular Zn2+ Dysregulation

  • Haruna Tamano
  • Yuichi Sato
  • Mako Takiguchi
  • Taku Murakami
  • Toshiyuki Fukuda
  • Hirokazu Kawagishi
  • Miki Suzuki
  • Atsushi TakedaEmail author
Original Research
  • 90 Downloads

Abstract

Exposure to corticosterone attenuates hippocampal CA1 long-term potentiation (LTP) via intracellular Zn2+ dysregulation. Here we report that effusol, a phenanthrene isolated from Chinese medicine Juncus effusus, rescues CA1 LTP attenuated by corticosterone. In vivo microdialysis experiment indicated that both increases in extracellular glutamate induced under perfusion with corticosterone and high K+ are suppressed in the hippocampus by co-perfusion with effusol. Because corticosterone and high K+ also increase extracellular Zn2+ level, followed by intracellular Zn2+ dysregulation, the effect of effusol on both the increases was examined in brain slice experiments. Effusol did not suppress increase in extracellular Zn2+ in the hippocampal CA1 of brain slices bathed in corticosterone, but suppressed increase in intracellular Zn2+, which may be linked with suppressing the increase in extracellular glutamate in vivo. In vivo CA1 LTP was attenuated under perfusion with corticosterone prior to LTP induction, while the attenuation was rescued by co-perfusion with effusol, suggesting that the rescuing effect of effusol is due to suppressing the increase in intracellular Zn2+ in CA1 pyramidal cells. The present study indicates that CA1 LTP attenuated by corticosterone is canceled by effusol, which rescues intracellular Zn2+ dysregulation via suppressing extracellular glutamate accumulation. It is likely that effusol defends the hippocampal function against stress-induced cognitive decline.

Keywords

Effusol Juncus effusus Zn2+ Corticosterone Hippocampus Stress 

Abbreviations

LTP

Long-term potentiation

MC

Mineralocorticoid

GC

Glucocorticoid

CS

Corticosterone

AMPA

α-Amino-3-hydroxy-5-methyl-4-isoxazolepropionate

GABA

γ-Aminobutyric acid

ACSF

Artificial cerebrospinal fluid

PCL

Pyramidal cell layer

SR

Stratum radiatum

Notes

Author Contributions

Planned experiments: H.T. and A.T. Performed the Experiments: Y.S., T.M, and T.F. Analyzed data: H.K. and M.S. Wrote the paper: H.T. and A.T. All authors reviewed the manuscript.

Compliance with Ethical Standards

Conflict of interest

All authors have no conflict of interest.

Ethical Approval

All applicable international, national, and/or institutional guidelines for the care and use of animals were followed.

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Department of Neurophysiology, School of Pharmaceutical SciencesUniversity of ShizuokaShizuokaJapan
  2. 2.Satoen CO., LTDShizuokaJapan
  3. 3.Research Institute of Green Science and TechnologyShizuoka UniversityShizuokaJapan

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