The Effect of MPP+ on the Viability of Primary Cortical Astrocytes Isolated from Female and Male Wistar Rats of Different Ages
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Although age is known to be the main risk for developing chronic and neurodegenerative diseases, those illnesses have a different prevalence depending on the sex. It has been questioned whether genetic and hormonal differences are preserved in primary cultures from individuals of different genders. Therefore, here we studied the susceptibility of astrocytes, obtained from female and male Wistar rats of different ages (newborn, 9 and 24 months-old), to the well-known toxin MPP+ after 2 weeks in vitro, at different concentrations and exposure times. Our results showed that there are no variances due to gender, but that there are important differences associated to age in terms of the viability against this toxin.
KeywordsAstrocytes Rats Sex Gender Age MPP+
The authors thank Dr. Wilhelmina Sudarsky for English editing. This work was supported by CONACyT grant FON.INST/298/2016.
Alarcón-Aguilar A and Luna-López A performed the experiments and statistics. López-Diazguerrero NE, Alarcón-Aguilar A and Konigsberg M designed the experiments, analyzed the data, and wrote the paper.
Compliance with Ethical Standards
Conflict of interest
The authors have no conflicts of interest to disclose.
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