Early Postnatal Development of the Lamination in the Lateral Geniculate Nucleus A-Layers in Cats
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The early postnatal development of the A-layers of the dorsal lateral geniculate nucleus (LGNd) was investigated in kittens aged 0–34 days by immunohistochemistry for the selective marker for neuronal differentiation (NeuN protein) and parvalbumin. We report two new facts about the LGNd development. First, there is a transient stratification of NeuN labelling in layer A, and to a lesser extent in layer A1, in kittens aged 0 and 4 days. Second, a transient population of large cells that are located between the LGNd A-layers (interlaminar cells) showed high expression levels of both NeuN and parvalbumin. These neurons possessed both the morphological and immunohistochemical features, similar to cells in the neighbouring perigeniculate nucleus. Both NeuN-stratification and double-stained interlaminar cells gradually disappeared during the second postnatal week, and almost completely vanished by the opening of the critical period. We discuss a possible linkage between these observed transitory networks and the ON-/OFF- and X-/Y-cells development and propose that the data obtained reflect the functioning of the early environmentally independent geniculate networks.
KeywordsLateral geniculate nucleus Cat Postnatal development NeuN Parvalbumin
The study was supported by the Russian Foundation for Basic Research (RFBR Grant No. 16-04-01791). The authors thank the Centre for Molecular and Cell Technologies, Research Park, Saint Petersburg State University, for supporting this research. The authors also thank Nikitina Nina for animal care and Schkorbatova Polina for help with immunostaining.
All authors had full access to all the data in the study and take responsibility for the integrity of the data and the accuracy of the data analysis. Study concept and design: MN. Acquisition of data: MN, MA. Analysis and interpretation of data: MN, MA. Drafting of the manuscript: MN, MA. Critical revision of the manuscript for important intellectual content: MN, MA. Statistical analysis: MN, MA. Obtained funding: MN, MA. Administrative, technical, and material support: MN, MA, ZP. Study supervision: MN.
Compliance with Ethical Standards
Conflict of interest
The authors report no conflicts of interest.
- Sanchez-Vives MV, Bal T, Kim U et al (1996) Are the interlaminar zones of the ferret dorsal lateral geniculate nucleus actually part of the perigeniculate nucleus? J Neurosci 16:5923–5941. https://doi.org/10.1523/JNEUROSCI.16-19-05923.1996 CrossRefPubMedGoogle Scholar
- Shatz CJ (1983) The prenatal development of the cat’s retinogeniculate pathway. J Neurosci 3:482–499. https://doi.org/10.1523/JNEUROSCI.03-03-00482.1983 CrossRefPubMedGoogle Scholar
- Sretavan DW, Shatz CJ (1986) Prenatal development of retinal ganglion cell axons: segregation into eye-specific layers within the cat’s lateral geniculate nucleus. J Neurosci 6:234–251. https://doi.org/10.1523/JNEUROSCI.06-01-00234.1986 CrossRefPubMedGoogle Scholar
- Stryker MP, Zahs KR (1983) On and off sublaminae in the lateral geniculate nucleus of the ferret. J Neurosci 3:1943–1951. https://doi.org/10.1523/JNEUROSCI.03-10-01943.1983 CrossRefPubMedGoogle Scholar