Cellular and Molecular Neurobiology

, Volume 38, Issue 2, pp 487–496 | Cite as

The Role of Peripheral Myelin Protein 2 in Remyelination

  • Mark StettnerEmail author
  • Jennifer Zenker
  • Fabian Klingler
  • Fabian Szepanowski
  • Hans-P. Hartung
  • Anne K. Mausberg
  • Christoph Kleinschnitz
  • Roman Chrast
  • Bernd C. Kieseier
Original Research


The protein component of the myelin layer is essential for all aspects of peripheral nerves, and its deficiency can lead to structural and functional impairment. The presence of peripheral myelin protein 2 (P2, PMP2, FABP8, M-FABP) in Schwann cells has been known for decades and shown recently to be involved in the lipid homeostasis in the peripheral neural system. However, its precise role during de- and remyelination has yet to be elucidated. To this end, we assessed remyelination after sciatic nerve crush injury in vivo, and in an experimental de/remyelination ex vivo myelinating culture model in P2-deficient (P2 −/− ) and wild-type (WT) animals. In vivo, the nerve crush paradigm revealed temporal structural and functional changes in P2 −/− mice as compared to WT animals. Concomitantly, P2 −/− DRG cultures demonstrated the presence of shorter internodes and enlarged nodes after ex vivo de/remyelination. Together, these data indicate that P2 may play a role in remyelination of the injured peripheral nervous system, presumably by affecting the nodal and internodal configuration.


Peripheral myelin P2 Neuropathy Nerve crush injury Dorsal root ganglia Chronic inflammatory demyelinating neuropathy CIDP 





Distal motor latency


Maximum compound muscle action potential




Dorsal root ganglia


Fatty acid binding protein




Glyceraldehyde-3-phosphate dehydrogenase


Myelin basic protein


Major histocompatibility complex


Nerve conduction velocity


Protein zero


Peripheral myelin protein 2




Room temperature


Real-time polymerase chain reaction


Sciatic nerve crush injury





The excellent technical assistance of Zippora Kohne and Jean-Jacques Médard is greatly appreciated. We thank Kathleen Ingenhoven for her great support in the lab.


No funding was received for the current study.

Authors’ Contributions

Study concept and design: MS, and BK. Acquisition of data: MS, FK, FS, JPW, TD, AKM. Analysis and interpretation of data: MS, JZ, RC, BCK. Drafting of the manuscript: MS, and BK. Critical revision of the manuscript for important intellectual content: MS, BK, JZ, RC, CK. Statistical analysis: MS, FK, FS, AKM. BK. Study supervision: MS, and BK. All authors have read and approved the final version of the manuscript.

Compliance with Ethical Standards

Competing interest

The authors declare that they have no competing interests.

Informed Consent

The manuscript does not contain any individual person’s data in any form. Not applicable.

Ethical Approval

All experiments implicating animals were performed in accordance with the legal requirements of the University of Düsseldorf and the “Landesamt für Natur- und Umweltschutz Nordrhein-Westfalen” (Regional Office for Nature, Environment and Consumer Protection), Germany.


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Copyright information

© Springer Science+Business Media New York 2017

Authors and Affiliations

  • Mark Stettner
    • 1
    Email author
  • Jennifer Zenker
    • 3
  • Fabian Klingler
    • 2
  • Fabian Szepanowski
    • 1
  • Hans-P. Hartung
    • 2
  • Anne K. Mausberg
    • 1
  • Christoph Kleinschnitz
    • 1
  • Roman Chrast
    • 4
  • Bernd C. Kieseier
    • 2
  1. 1.Department of NeurologyUniversity Hospital EssenEssenGermany
  2. 2.Department of NeurologyHeinrich-Heine-UniversityDüsseldorfGermany
  3. 3.Institute of Molecular and Cell Biology, A*STARSingaporeSingapore
  4. 4.Department of Neuroscience and Department of Clinical NeuroscienceKarolinska InstitutetStockholmSweden

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