The Role of Peripheral Myelin Protein 2 in Remyelination
- 501 Downloads
The protein component of the myelin layer is essential for all aspects of peripheral nerves, and its deficiency can lead to structural and functional impairment. The presence of peripheral myelin protein 2 (P2, PMP2, FABP8, M-FABP) in Schwann cells has been known for decades and shown recently to be involved in the lipid homeostasis in the peripheral neural system. However, its precise role during de- and remyelination has yet to be elucidated. To this end, we assessed remyelination after sciatic nerve crush injury in vivo, and in an experimental de/remyelination ex vivo myelinating culture model in P2-deficient (P2 −/− ) and wild-type (WT) animals. In vivo, the nerve crush paradigm revealed temporal structural and functional changes in P2 −/− mice as compared to WT animals. Concomitantly, P2 −/− DRG cultures demonstrated the presence of shorter internodes and enlarged nodes after ex vivo de/remyelination. Together, these data indicate that P2 may play a role in remyelination of the injured peripheral nervous system, presumably by affecting the nodal and internodal configuration.
KeywordsPeripheral myelin P2 Neuropathy Nerve crush injury Dorsal root ganglia Chronic inflammatory demyelinating neuropathy CIDP
Distal motor latency
Maximum compound muscle action potential
Dorsal root ganglia
Fatty acid binding protein
Myelin basic protein
Major histocompatibility complex
Nerve conduction velocity
Peripheral myelin protein 2
Real-time polymerase chain reaction
Sciatic nerve crush injury
The excellent technical assistance of Zippora Kohne and Jean-Jacques Médard is greatly appreciated. We thank Kathleen Ingenhoven for her great support in the lab.
No funding was received for the current study.
Study concept and design: MS, and BK. Acquisition of data: MS, FK, FS, JPW, TD, AKM. Analysis and interpretation of data: MS, JZ, RC, BCK. Drafting of the manuscript: MS, and BK. Critical revision of the manuscript for important intellectual content: MS, BK, JZ, RC, CK. Statistical analysis: MS, FK, FS, AKM. BK. Study supervision: MS, and BK. All authors have read and approved the final version of the manuscript.
Compliance with Ethical Standards
The authors declare that they have no competing interests.
The manuscript does not contain any individual person’s data in any form. Not applicable.
All experiments implicating animals were performed in accordance with the legal requirements of the University of Düsseldorf and the “Landesamt für Natur- und Umweltschutz Nordrhein-Westfalen” (Regional Office for Nature, Environment and Consumer Protection), Germany.
- Lehmann HC et al (2009) Matrix metalloproteinase-2 is involved in myelination of dorsal root ganglia neurons Glia 57:479–489 doi: 10.1002/glia.20774
- Ruskamo S et al (2014) Atomic resolution view into the structure-function relationships of the human myelin peripheral membrane protein P2. Acta Crystallogr 70:165–176Google Scholar
- Trapp BD, McIntyre LJ, Quarles RH, Sternberger NH, Webster HD (1979) Immunocytochemical localization of rat peripheral nervous system myelin proteins: P2 protein is not a component of all peripheral nervous system myelin sheaths. Proc Natl Acad Sci USA 76:3552–3556CrossRefPubMedPubMedCentralGoogle Scholar