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Cellular and Molecular Neurobiology

, Volume 37, Issue 1, pp 29–36 | Cite as

Atorvastatin Upregulates the Expression of miR-126 in Apolipoprotein E-knockout Mice with Carotid Atherosclerotic Plaque

  • Xudong Pan
  • Rongyao Hou
  • Aijun MaEmail author
  • Ting Wang
  • Mei Wu
  • Xiaoyan Zhu
  • Shaonan Yang
  • Xing Xiao
Original Research

Abstract

Carotid atherosclerosis (AS) is a chronic inflammatory disease of the carotid arterial wall, which is very important in terms of the occurrence of cerebral vascular accidents. Studies have demonstrated that microRNAs (miRNAs) and their target genes are involved in the formation of atherosclerosis and that atorvastatin might reduce atherosclerotic plaques by regulating the expression of miRNAs. However, the related mechanism is not yet known. In this study, we first investigated the effects of atorvastatin on miR-126 and its target gene, i.e., vascular cell adhesion molecule-1 (VCAM-1) in apolipoprotein E-knockout (ApoE−/−) mice with carotid atherosclerotic plaque in vivo. We compared the expressions of miR-126 and VCAM-1 between the control, atherosclerotic model and atorvastatin treatment groups of ApoE−/− mice using RT-PCR and Western blot. We found the miR-126 expression was significantly down-regulated, and the VCAM-1 expression was significantly up-regulated in the atherosclerotic model group, which accelerated the progression of atherosclerosis in the ApoE−/− mice. These results following atorvastatin treatment indicated that miR-126 expression was significantly up-regulated, VCAM-1 expression was significantly down-regulated and atherosclerotic lesions were reduced. The present results might explain the mechanism by which miR-126 is involved in the formation of atherosclerosis in vivo. Our study first indicated that atorvastatin might exert its anti-inflammatory effects in atherosclerosis by regulating the expressions of miR-126 and VCAM-1 in vivo.

Keywords

Carotid atherosclerosis miR-126 VCAM-1 Atorvastatin Apolipoprotein E-knockout mice 

Notes

Acknowledgments

The authors gratefully acknowledge the financial support from the National Natural Sciences Foundation of China (No. 81571112).

Compliance with Ethical Standards

Conflict of interest

We declare that we have no financial or personal relationships with other people or organizations that could have inappropriately influenced our work. There are no professional or other personal interests of any nature or type in any product, service and/or company that could be construed as influencing the positions presented in this manuscript.

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Copyright information

© Springer Science+Business Media New York 2016

Authors and Affiliations

  • Xudong Pan
    • 1
  • Rongyao Hou
    • 2
  • Aijun Ma
    • 1
    Email author
  • Ting Wang
    • 1
  • Mei Wu
    • 3
  • Xiaoyan Zhu
    • 4
  • Shaonan Yang
    • 1
  • Xing Xiao
    • 1
  1. 1.Department of NeurologyThe Affiliated Hospital of Qingdao UniversityQingdaoPeople’s Republic of China
  2. 2.Department of NeurologyThe Affiliated Hiser Hospital of Qingdao UniversityQingdaoPeople’s Republic of China
  3. 3.Laboratory of Human Micromorphologythe Medical College of Qingdao UniversityQingdaoPeople’s Republic of China
  4. 4.Department of Critical Care Medicinethe Affiliated Hiser Hospital of Qingdao UniversityQingdaoPeople’s Republic of China

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