Cellular and Molecular Neurobiology

, Volume 35, Issue 7, pp 931–941 | Cite as

Mitochondrial Cyclophilin D as a Potential Therapeutic Target for Ischemia-Induced Facial Palsy in Rats

  • Huizhen Chen
  • Chnagtao Liu
  • Jie Yin
  • Zhen Chen
  • Jinwang Xu
  • Duanlei Wang
  • Jiaqiu Zhu
  • Ziyuan Zhang
  • Yong Sun
  • Aimin Li
Original Research
First Online:
Received:
Accepted:

Abstract

Many studies have demonstrated that ischemia could induce facial nerve (FN) injury. However, there is a lack of a suitable animal model for FN injury study and thus little knowledge is available about the precise mechanism for FN injury. The aims of this study were to establish a reliable FN injury model induced by blocking the petrosal artery and to investigate whether dysfunctional interaction between cyclophilin D (CypD) and mitochondrial permeability transition pore (MPTP) can mediate cell dysfunction in ischemic FN injury. The outcomes of ischemia-induced FN injury rat model were evaluated by behavioral assessment, histological observation, electrophysiology, and electron microscopy. Then the levels of CypD and protein that forms the MPTP were evaluated under the conditions with or without the treatment of Cyclosporin A (CsA), which has been found to disrupt MPTP through the binding of CypD. The blocking of petrosal artery caused significant facial palsy signs in the ischemia group but not in the sham group. Furthermore, ischemia can induce the dysfunction of facial nucleus neurons and destruction of the myelin sheath and increase the protein levels of CypD and MPTP protein compared with sham group. Interestingly, treatment with CsA significantly improved neurological function and reversed the ischemia-induced increase of CypD and MPTP proteins in ischemia group. These results demonstrated that blocking of petrosal artery in rats can induce FN injury and the mechanism may be related to the disruption of MPTP by CypD.

Keywords

Facial nerve Animal model Ischemia CsA CypD MPTP 
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Notes

Acknowledgments

This work was supported by the “333” Project of Jiangsu Province (BAR2010050).

Conflict of interest

The authors declare no competing financial interests.

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Copyright information

© Springer Science+Business Media New York 2015

Authors and Affiliations

  • Huizhen Chen
    • 1
  • Chnagtao Liu
    • 1
  • Jie Yin
    • 2
  • Zhen Chen
    • 1
  • Jinwang Xu
    • 1
  • Duanlei Wang
    • 1
  • Jiaqiu Zhu
    • 1
  • Ziyuan Zhang
    • 1
  • Yong Sun
    • 1
  • Aimin Li
    • 1
  1. 1.Department of NeurosurgeryThe First People’s Hospital of LianyungangLianyungangPeople’s Republic of China
  2. 2.Department of NeurosurgeryXuzhou Central HospitalXuzhouPeople’s Republic of China

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