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Cellular and Molecular Neurobiology

, Volume 35, Issue 4, pp 513–522 | Cite as

Resveratrol Protects PC12 Cells from High Glucose-Induced Neurotoxicity Via PI3K/Akt/FoxO3a Pathway

  • Mi-Hua Liu
  • Cong Yuan
  • Jun He
  • Tian-Ping Tan
  • Shao-Jian Wu
  • Hong-Yun Fu
  • Jun Liu
  • Shan Yu
  • Yu-Dan Chen
  • Qun-Fang Le
  • Wei Tian
  • Heng-Jing Hu
  • Yuan Zhang
  • Xiao-Long Lin
Original Research

Abstract

Diabetes is known to be associated with neurodegenerative diseases. Resveratrol, a plant-derived polyphenolic compound found in red wine, possesses antioxidant properties. In this study, we aimed to investigate the effects of resveratrol on the phosphatidylinositol-3-kinase/protein kinase B (PI3K/Akt)/FoxO3a pathway in mediating high glucose (HG)-induced injuries in neuronal PC12 cells. PC12 cells were exposed to HG to establish a model of HG neurotoxicity. Results showed that pre-treating PC12 cells with resveratrol before exposure to HG led to increased cell viability, decreased apoptotic cells, and reactive oxygen species generation. Western blot analysis showed that HG decreased the phosphorylation of Akt and FoxO3a and led to the nuclear localization of FoxO3a. These effects were significantly alleviated by resveratrol co-treatment. Furthermore, the protective effects of resveratrol were abolished by PI3K/Akt inhibitor LY294002. All these results demonstrate that resveratrol protected the PC12 cells from HG-induced oxidative stress and apoptosis via the activation of PI3K/Akt/FoxO3a signaling pathway.

Keywords

Resveratrol High glucose Oxidative stress Apoptosis FoxO3a 

Notes

Acknowledgments

This work was supported by grants from Medical scientific research funds of Guangdong Province (A2014810) and Graduate student research innovation project of Hunan Province (CX2013B397).

Conflict of interest

The authors have no conflict of interest.

Supplementary material

10571_2014_147_MOESM1_ESM.tif (275 kb)
Supplementary material 1 (TIFF 275 kb)

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Copyright information

© Springer Science+Business Media New York 2014

Authors and Affiliations

  • Mi-Hua Liu
    • 1
  • Cong Yuan
    • 3
  • Jun He
    • 1
  • Tian-Ping Tan
    • 1
  • Shao-Jian Wu
    • 1
  • Hong-Yun Fu
    • 1
  • Jun Liu
    • 1
  • Shan Yu
    • 1
  • Yu-Dan Chen
    • 1
  • Qun-Fang Le
    • 1
  • Wei Tian
    • 1
  • Heng-Jing Hu
    • 4
  • Yuan Zhang
    • 5
  • Xiao-Long Lin
    • 2
  1. 1.Department of Clinical Laboratory, Affiliated Nanhua HospitalUniversity of South ChinaHengyangPeople’s Republic of China
  2. 2.Department of PathologyThe Third People’s Hospital of HuizhouHuizhou CityPeople’s Republic of China
  3. 3.Department of CardiologyThe First Hospital of ChangshaChangsha CityPeople’s Republic of China
  4. 4.Department of Cardiology/Cardiac Catheterisation Lab, Second Xiangya HospitalCentral South UniversityChangsha CityPeople’s Republic of China
  5. 5.Department of PathologyMawangdui HospitalChangsha CityPeople’s Republic of China

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