Cellular and Molecular Neurobiology

, Volume 34, Issue 2, pp 289–296 | Cite as

DRG Axon Elongation and Growth Cone Collapse Rate Induced by Sema3A are Differently Dependent on NGF Concentration

  • Andrius Kaselis
  • Rimantas Treinys
  • Rūta Vosyliūtė
  • Saulius Šatkauskas
Original Research

Abstract

Regeneration of embryonic and adult dorsal root ganglion (DRG) sensory axons is highly impeded when they encounter neuronal growth cone-collapsing factor semaphorin3A (Sema3A). On the other hand, increasing evidence shows that DRG axon’s regeneration can be stimulated by nerve growth factor (NGF). In this study, we aimed to evaluate whether increased NGF concentrations can counterweight Sema3A-induced inhibitory responses in 15-day-old mouse embryo (E15) DRG axons. The DRG explants were grown in Neurobasal-based medium with different NGF concentrations ranging from 0 to 100 ng/mL and then treated with Sema3A at constant 10 ng/mL concentration. To evaluate interplay between NGF and Sema3A number of DRG axons, axon outgrowth distance and collapse rate were measured. We found that the increased NGF concentrations abolish Sema3A-induced inhibitory effect on axon outgrowth, while they have no effect on Sema3A-induced collapse rate.

Keywords

Semaphorin Sema3A DRG Axon growth NGF Growth cone collapse 

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Copyright information

© Springer Science+Business Media New York 2013

Authors and Affiliations

  • Andrius Kaselis
    • 1
  • Rimantas Treinys
    • 1
    • 2
  • Rūta Vosyliūtė
    • 1
  • Saulius Šatkauskas
    • 1
  1. 1.Biophysical Research Group, Biology DepartmentVytautas Magnus UniversityKaunasLithuania
  2. 2.Institute of CardiologyLithuanian University of Health SciencesKaunasLithuania

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