Cellular and Molecular Neurobiology

, Volume 33, Issue 3, pp 369–377

Sexual Dimorphism in Expression of Insulin and Insulin-Like Growth Factor-I Receptors in Developing Rat Cerebellum

  • Hossein Haghir
  • Abd-Al-Rahim Rezaee
  • Hossein Nomani
  • Mojtaba Sankian
  • Hamed Kheradmand
  • Javad Hami
Original Research


The insulin and insulin-like growth factor-1 (IGF-1) are considered to play important roles in brain development; and their cognate receptors -InsR and IGF-1R- localized within distinct brain regions including cerebellum. Using Real-Time PCR and western blot analysis, we compared the expression of InsR and IGF-1R in male and female developing rat cerebellum at P0, P7, and P14. At all time points studied, the cerebellar expression of IGF-1R, both at mRNA and protein levels was higher than that of InsR. The lowest InsR and IGF-1R mRNA and protein levels were measured in the neonate cerebellum, independent of gender. In males, the highest InsR and IGF-1R mRNA and protein expression were found at P7. InsR and IGF-1R expression increased significantly between P0 and P7, followed by a marked downregulation at P14. In contrast, in females, mRNA and protein levels of InsR and IGF-1R remain unchanged between P0 and P7, and are upregulated at P14. Therefore, peaked InsR and IGF-1R expression in female cerebelli occurred at P14. Interestingly, changes in mRNA expression and in protein levels followed the same developmental pattern, indicating that InsR and IGF-1R transcription is not subject to modulatory effects during the first 2 weeks of development. These findings indicate that there are prominent sexual differences in InsR and IGF-1R expression in the developing rat cerebellum, suggesting a probable mechanism for the control of gender differences in development and function of the cerebellum.


Insulin-like growth factor 1 receptor Insulin receptor Cerebellum Rat newborn 


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Copyright information

© Springer Science+Business Media New York 2013

Authors and Affiliations

  • Hossein Haghir
    • 1
    • 2
  • Abd-Al-Rahim Rezaee
    • 3
  • Hossein Nomani
    • 3
  • Mojtaba Sankian
    • 4
  • Hamed Kheradmand
    • 5
  • Javad Hami
    • 6
  1. 1.Department of Anatomy and Cell BiologySchool of Medicine, Mashhad University of Medical SciencesMashhadIran
  2. 2.Medical Genetics and Functional Genomics Research CenterSchool of Medicine, Mashhad University of Medical SciencesMashhadIran
  3. 3.Microbiology and Virology Research CenterQaem Hospital, Mashhad University of Medical SciencesMashhadIran
  4. 4.Immunology Research Center, Bu-Ali Research InstituteSchool of Medicine, Mashhad University of Medical SciencesMashhadIran
  5. 5.Hazrat Rasoul Hospital, Tehran University of Medical SciencesTehranIran
  6. 6.Department of AnatomySchool of Medicine, Birjand University of Medical SciencesBirjandIran

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