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Cellular and Molecular Neurobiology

, Volume 32, Issue 7, pp 1089–1097 | Cite as

Neurotrophin Expressions in Neural Stem Cells Grafted Acutely to Transected Spinal Cord of Adult Rats Linked to Functional Improvement

  • Ying-Li Gu
  • Lu-Wei Yin
  • Zhuo Zhang
  • Jia Liu
  • Su-Juan Liu
  • Lian-Feng Zhang
  • Ting-Hua WangEmail author
Original Research

Abstract

It is well known that neural stem cells (NSC) could promote the repairment after spinal cord injury, but the underlying mechanism remains to be elucidated. This study showed that the transplantation of NSC significantly improved hindlimb locomotor functions in adult rats subjected to transection of the spinal cord. Biotin dextran amine tracing together with the stimulus experiment in motor sensory area showed that little CST regeneration existed and functional synaptic formation in the injury site. Immunocytochemistry and RT-PCR demonstrated the secretion of NGF, BDNF, and NT-3 by NSC in vitro and in vivo, respectively. However, only mRNA expression of BDNF and NT-3 but not NGF in injury segment following NSC transplantation was upregulated remarkably, while caspase-3, a crucial apoptosis gene, was downregulated simultaneously. These provided us a clue that the functional recovery was correlated with the regulation of BDNF, NT-3, and caspase-3 in spinal cord transected rats following NSC transplantation.

Keywords

NSC Transplantation Sensory and locomotor functions Rat 

Notes

Acknowledgments

We thank Professor Seng-Kee Leong for his valuable comments on this manuscript. This research was supported by a grant from the China National Science Foundation (No. 30260125) and the New York-China Medical Board (CMB00-722).

Conflict of interest

We have no conflicts of interest in this study.

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Copyright information

© Springer Science+Business Media, LLC 2012

Authors and Affiliations

  • Ying-Li Gu
    • 1
  • Lu-Wei Yin
    • 2
  • Zhuo Zhang
    • 5
  • Jia Liu
    • 2
  • Su-Juan Liu
    • 4
  • Lian-Feng Zhang
    • 3
  • Ting-Hua Wang
    • 2
    • 4
    Email author
  1. 1.Department of Neurology, West China HospitalSichuan UniversityChengduChina
  2. 2.Institute of Neuroscience, Kunming Medical CollegeKunmingChina
  3. 3.Institute of Laboratory Animal ScienceCAMSBeijingChina
  4. 4.Translational Neuroscience CenterInstitute of Neurological Disease, West China Hospital, Sichuan UniversityChengduChina
  5. 5.Department of NeurologyTangdu Hospital, Fourth Military Medical UniversityXi’anChina

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