Neurotrophin Expressions in Neural Stem Cells Grafted Acutely to Transected Spinal Cord of Adult Rats Linked to Functional Improvement
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It is well known that neural stem cells (NSC) could promote the repairment after spinal cord injury, but the underlying mechanism remains to be elucidated. This study showed that the transplantation of NSC significantly improved hindlimb locomotor functions in adult rats subjected to transection of the spinal cord. Biotin dextran amine tracing together with the stimulus experiment in motor sensory area showed that little CST regeneration existed and functional synaptic formation in the injury site. Immunocytochemistry and RT-PCR demonstrated the secretion of NGF, BDNF, and NT-3 by NSC in vitro and in vivo, respectively. However, only mRNA expression of BDNF and NT-3 but not NGF in injury segment following NSC transplantation was upregulated remarkably, while caspase-3, a crucial apoptosis gene, was downregulated simultaneously. These provided us a clue that the functional recovery was correlated with the regulation of BDNF, NT-3, and caspase-3 in spinal cord transected rats following NSC transplantation.
KeywordsNSC Transplantation Sensory and locomotor functions Rat
We thank Professor Seng-Kee Leong for his valuable comments on this manuscript. This research was supported by a grant from the China National Science Foundation (No. 30260125) and the New York-China Medical Board (CMB00-722).
Conflict of interest
We have no conflicts of interest in this study.
- Klapka N, Hermanns S, Straten G, Masanneck C, Duis S, Hamers FP, Müller D, Zuschratter W, Müller HW (2005) Suppression of fibrous scarring in spinal cord injury of rat promotes long-distance regeneration of corticospinal tract axons, rescue of primary motoneurons in somatosensory cortex and significant functional recovery. Eur J Neurosci 22:3047–3058PubMedCrossRefGoogle Scholar
- Li SH, Hou TS (2001) The impact of direct-current stimulation on apoptotic adjustable gene expression of spinal cord motorneurons after sciatic nerve transection. Chin J Traumatol 17:398–401Google Scholar
- Xie YB, Niu YJ, Yuan CY, Ying Y, Yu YT (2007) Effects of BDNF on the expression of caspase-2 and caspase-3 and cell apoptosis in retinal ischemia/reperfusion injury. Int J Ophthalmol 7:1217–1221Google Scholar