Protective Effect of Bacopa monniera on Methyl Mercury-Induced Oxidative Stress in Cerebellum of Rats
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Methyl mercury (MeHg) is a ubiquitous environmental pollutant leading to neurological and developmental deficits in animals and human beings. Bacopa monniera (BM) is a perennial herb and is used as a nerve tonic in Ayurveda, a traditional medicine system in India. The objective of the present study was to investigate whether Bacopa monniera extract (BME) could potentially inhibit MeHg-induced toxicity in the cerebellum of rat brain. Male Wistar rats were administered with MeHg orally at a dose of 5 mg/kg b.w. for 21 days. Experimental rats were given MeHg and also administered with BME (40 mg/kg, orally) for 21 days. After the treatment period, we observed that MeHg exposure significantly inhibited the activities of superoxide dismutase, catalase, glutathione peroxidase, and increased the glutathione reductase activity in cerebellum. It was also found that the level of thiobarbituric acid-reactive substances was increased with the concomitant decrease in the glutathione level in MeHg-induced rats. These alterations were prevented by the administration of BME. Behavioral interference in the MeHg-exposed animals was evident through a marked deficit in the motor performance in the rotarod task, which was completely recovered to control the levels by BME administration. The total mercury content in the cerebellum of MeHg-induced rats was also increased which was measured by atomic absorption spectrometry. The levels of NO2 − and NO3 − in the serum were found to be significantly increased in the MeHg-induced rats, whereas treatment with BME significantly decreased their levels in serum to near normal when compared to MeHg-induced rats. These findings strongly implicate that BM has potential to protect brain from oxidative damage resulting from MeHg-induced neurotoxicity in rat.
KeywordsMethyl mercury Bacopamonniera Cerebellum Neuroprotection
The work described in this article was supported by Department of Medical Biochemistry, DR. ALMPGIBMS, University of Madras, Taramani Campus, Chennai 113, Tamil Nadu, India.
Conflict of interest
The authors declare that there is no conflict of interests.
- Bhattacharya SK, Kumar A, Ghosal S (1999) Effect of Bacopa monniera on animal models of Alzheimer’s disease and perturbed central cholinergic markers of cognition in rats. Res Commun Pharmacol Toxicol 4:1–12Google Scholar
- Duham NW, Miya TS (1957) A note on a simple apparatus for detecting neurological deficit in rats and mice. J Am Pharm Assoc 46:208–209Google Scholar
- Hunter D, Bomford RR, Russell DS (1940) Poisoning by methylmercury compounds. Quart J Med 9:193–213Google Scholar
- Paxinos G, Watson C (1982) The rat brain in sterotaxic coordinates. Academic Press, New YorkGoogle Scholar
- Shanker G, Singh HK (2000) Anxiolytic profile of standardized Brahmi extract. Indian J Pharmacol 32:152Google Scholar
- Sharma R, Chaturvedi C, Tewari PV (1987) Efficacy of Bacopa monniera in revitalizing intellectual functions in children. J Res Edu Ind Med 1:12Google Scholar
- Singh HK, Dhawan BN (1997) Neuropsychopharmacological effects of the ayurvedic nootropic Bacopa monniera Linn (Brahmi). Indian J Pharmacol 29:359–365Google Scholar
- Takeuchi T (1968) Pathology of minamata disease. In: Kutsuna M (ed) Study group of minamata disease. Kumamoto University, Shuhan Publisher, Tokyo, pp 141–228Google Scholar