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Cellular and Molecular Neurobiology

, Volume 32, Issue 4, pp 577–586 | Cite as

Changes of Ribosomal Protein S3 Immunoreactivity and Its New Expression in Microglia in the Mice Hippocampus After Lipopolysaccharide Treatment

  • Hui Young Lee
  • Joon Ha Park
  • Choong Hyun LeeEmail author
  • Bingchun Yan
  • Ji Hyeon Ahn
  • Young Joo Lee
  • Chan Woo Park
  • Jun Hwi Cho
  • Soo Young Choi
  • Moo-Ho WonEmail author
Original Research

Abstract

Lipopolysaccharide (LPS) has been used as a reagent for a model of systemic inflammatory response. Ribosomal protein S3 (rpS3) is a multi-functional protein that is involved in transcription, metastasis, DNA repair, and apoptosis. In the present study, we examined the changes of rpS3 immunoreactivity in the mouse hippocampus after systemic administration of 1 mg/kg of LPS. From 6 h after LPS treatment, rpS3 immunoreactivity was decreased in pyramidale cells of the hippocampus proper (CA1–CA3 regions) and in granule cells of the dentate gyrus. At this point in time, rpS3 immunoreactivity began to increase in non-pyramidal cells and non-granule cells. From 1 day after LPS treatment, rpS3 immunoreactivity in pyramidal and granule cells was hardly detected; however, strong rpS3 immunoreactivity was shown in non-pyramidal and non-granule cells. Based on double immunofluorescence staining for rpS3/ionized calcium-binding adapter 1 (Iba-1, a marker for microglia) and glial fibrillary acidic protein (GFAP, a marker for astrocytes), strong rpS3 immunoreactivity was expressed in Iba-1-immunoreactive microglia, not in GFAP-immunoreactive astrocytes, at 1 and 2 days after LPS treatment. These results indicate that rpS3 immunoreactivity changes only in pyramidal and granule cells, and rpS3 is expressed only in activated microglia after LPS treatment: this may be associated with the neuroinflammatory responses in the brain.

Keywords

Multi-functional protein Lipopolysaccharide Neuroinflammation Glia 

Notes

Acknowledgment

The authors would like to thank Mr. Seung Uk Lee for their technical help in this study. This study was supported by Mid-career Researcher Program from the NRF Grant funded by the MEST (2009-0086319) and by 2011 Research Grant from Kangwon National University.

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Copyright information

© Springer Science+Business Media, LLC 2012

Authors and Affiliations

  • Hui Young Lee
    • 1
  • Joon Ha Park
    • 2
  • Choong Hyun Lee
    • 3
    Email author
  • Bingchun Yan
    • 2
  • Ji Hyeon Ahn
    • 4
  • Young Joo Lee
    • 5
  • Chan Woo Park
    • 6
  • Jun Hwi Cho
    • 6
  • Soo Young Choi
    • 7
  • Moo-Ho Won
    • 2
    Email author
  1. 1.Department of Internal Medicine, Institute of Medical Sciences, School of MedicineKangwon National UniversityChuncheonSouth Korea
  2. 2.Department of Neurobiology, Institute of Medical Sciences, School of MedicineKangwon National UniversityChuncheonSouth Korea
  3. 3.Department of Anatomy and Physiology, College of PharmacyDankook UniversityCheonanSouth Korea
  4. 4.Laboratory of Neuroscience, Department of Physical Therapy, College of Rehabilitation ScienceDaegu UniversityDaeguSouth Korea
  5. 5.Department of Emergency Medicine, Seoul Hospital, College of MedicineSooncheonhyang UniversitySeoulSouth Korea
  6. 6.Department of Emergency Medicine, Institute of Medical Sciences, School of MedicineKangwon National UniversityChuncheonSouth Korea
  7. 7.Department of Biomedical Sciences, Research Institute for Bioscience and BiotechnologyHallym UniversityChuncheonSouth Korea

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