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Cellular and Molecular Neurobiology

, Volume 32, Issue 2, pp 279–287 | Cite as

Existence of Tenascin-C Isoforms in Rat that Contain the Alternatively Spliced AD1 Domain are Developmentally Regulated During Hippocampal Development

  • J. GarwoodEmail author
  • U. Theocharidis
  • V. Calco
  • A. Dobbertin
  • A. FaissnerEmail author
Original Research

Abstract

Tenascin-C (TN-C) is a multimodular glycoprotein of the extracellular matrix which is important for the development of the nervous system and has a range of different functions which are mediated by the different protein domains present. TN-C contains eight constitutive fibronectin type III (FNIII) domains and a region of alternatively spliced FNIII domains. In the mouse and chick, six of these domains have been described and characterized, whereas in human there are nine of them. In this report, we show that seven alternatively spliced FNIII domains exist in rat and describe the differential expression pattern of the additional domain AD1 during embryonic and postnatal rat brain development. The AD1 domain of rat is homologous to the ones described in human and chick proteins but does not exist in mouse. Its expression can be located to the developing rat hippocampus and the lining of the lateral ventricle, regions where the TN-C protein may affect the behavior of stem and progenitor cells. During hippocampal development AD1 and the other alternatively spliced domains are differentially expressed as shown by RT-PCRs, immunocytochemistry and in situ hybridizations.

Keywords

Extracellular matrix Central nervous system Glycoprotein Neurite outgrowth 

Abbreviations

TN-C

Tenascin-C

FNIII

Fibronectin type III domain

RT-PCR

Reverse transcription-polymerase chain reaction

ECM

Extracellular matrix

CNS

Central nervous system

EGF

Epidermal growth factor

PDGF

Platelet-derived growth factor

VZ

Ventricular zone

CA

Cornu ammonis

MMP

Matrix metalloproteinase

TGF

Transforming growth factor

Notes

Acknowledgments

The authors thank Tim Czopka for the TN-C riboprobe, Anke Mommsen for excellent technical assistance, and Corinna Zimmermann for her help. This study was supported by the CNRS, German Research Council (DFG SPP, Molecular and Cellular Basis of CNS Repair Fa 159/11-1,2,3), the Association pour la Recherche contre le Cancer (ARC), and the International Spinal Research Trust (ISRT).

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Copyright information

© Springer Science+Business Media, LLC 2011

Authors and Affiliations

  1. 1.Department of Cell Morphology and Molecular NeurobiologyRuhr UniversityBochumGermany
  2. 2.Département Neurotransmission et Sécrétion Neuroendocrine, Institut des Neurosciences Cellulaires et Intégratives (UPR 3212)Centre National de la Recherche Scientifique et Université de StrasbourgStrasbourgFrance
  3. 3.Université Paris Descartes, INSERM U686, Centre universitaire des Saints-PèresParisFrance

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