Roles of Myosin Va and Rab3D in Membrane Remodeling of Immature Secretory Granules
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Neuroendocrine secretory granules (SGs) are formed at the trans-Golgi network (TGN) as immature intermediates. In PC12 cells, these immature SGs (ISGs) are transported within seconds to the cell cortex, where they move along actin filaments and complete maturation. This maturation process comprises acidification-dependent processing of cargo proteins, condensation of the SG matrix, and removal of membrane and proteins not destined to mature SGs (MSGs) into ISG-derived vesicles (IDVs). We investigated the roles of myosin Va and Rab3 isoforms in the maturation of ISGs in neuroendocrine PC12 cells. The expression of dominant-negative mutants of myosin Va or Rab3D blocked the removal of the endoprotease furin from ISGs. Furthermore, expression of mutant Rab3D, but not of mutant myosin Va, impaired cargo processing of SGs. In conclusion, our data suggest an implication of myosin Va and Rab3D in the maturation of SGs where they participate in overlapping but not identical tasks.
KeywordsDense core vesicle Endocrine Exocytosis Furin Hormone Maturation Myosin Va Noc2 Processing Rab3 Rab27 Secretory granule
Immature secretory granules
Dominant-negative Myosin Va-tail fragment
Mature secretory granules
Soluble N-ethylmaleimide-sensitive-factor attachment receptor
In exocrine, endocrine, and neuroendocrine cells secretory granules (SGs) are synthesized at trans-Golgi network (TGN) as short-lived vesicular intermediates. These immature secretory granules (ISGs) are converted to mature SGs (MSGs), the storage organelle of hormones and neuropeptides. This involves a complex maturation process, which includes further refinement of the content and remodeling of the organelle membrane (Tooze et al. 2001). One of the best-studied paradigms in this context, the maturation of SGs in neuroendocrine PC12 cells, was shown to occur in several consecutive/concurrent steps, which partly depend on each other. First, newly formed ISGs undergo homotypic fusion (Urbe et al. 1998) in a syntaxin 6- and synaptotagmin IV-dependent manner (Wendler et al. 2001; Ahras et al. 2006). Second, the lumen of ISGs acidifies progressively and thereby promotes enzymatic processing of precursor proteins and condensation of luminal cargo (Gerdes et al. 1989; Urbe et al. 1997; Seidah et al. 2008). Third, proteins not destined for MSGs along with excess membrane are removed from maturing ISGs in clathrin-coated immature secretory granule-derived vesicles (IDVs) (Tooze 1998). The proteins shown to reside in ISGs but not in MSGs include furin (Dittie et al. 1997), mannose-6-phosphate receptor (M6PR), syntaxin 6, clathrin (Klumperman et al. 1998), carboxypeptidase D (Varlamov et al. 1999), synaptotagmin IV, VAMP4 (Eaton et al. 2000), and Golgi-associated, γ-ear-containing, ADP-ribosylation factor-binding protein (GGA) (Kakhlon et al. 2006).
Mature secretory granules (MSGs), the product of the maturation process, are subject of additional modification steps leading to their tethering, priming, and docking (Sorensen 2004). During these events, MSGs are segregated into different pools with specific release probabilities (Neher and Zucker 1993; Burgoyne and Morgan 2003; Rose et al. 2003; Sorensen 2004). Upon calcium-dependent stimulation, the soluble N-ethylmaleimide-sensitive-factor attachment receptor (SNARE) complex mediates the fusion of SGs with the plasma membrane (Burgoyne and Morgan 2003). This final step in the life cycle of SGs is orchestrated by numerous accessory/regulatory proteins (Burgoyne and Morgan 2003) in a cell-type specific manner. These include munc13 and munc18 (Shirakawa et al. 2004; Nili et al. 2006), Rab3 and Rab27 (Fukuda 2008), synaptotagmin-like proteins (Desnos et al. 2003; Fukuda 2005b) and myosin Va (Rose et al. 2003; Varadi et al. 2003; Ivarsson et al. 2005; Watanabe et al. 2005; Desnos et al. 2007; Kogel et al. 2010). For myosin Va, two different roles in late trafficking steps have been proposed: transport of SGs along cortical actin fibers to the release sites (Rose et al. 2003) and docking of SGs at the cell cortex/plasma membrane (Watanabe et al. 2005; Desnos et al. 2007). Furthermore, recent studies on Rab3A and Rab27A suggest a role of both Rabs in the recruitment/tethering of SGs to the plasma membrane rather than in the priming and fusion step (Kasai et al. 2005; Tsuboi and Fukuda 2006). However, many open questions remain as to how these proteins control exocytosis of SGs. We have recently focused on potential functions of myosin Va and Rab 3 in early steps of SG trafficking and present evidence for an essential role of both proteins in SG maturation.
To analyze ISG maturation microscopically, we developed protocols to specifically label ISGs. This is necessary because only a small fraction of the entire SG population of neuroendocrine cells is immature due to their relatively short half-life (Tooze et al. 1991). We established a pulse-chase-like labeling of ISGs in PC12 cells, which was based on the expression of SG marker proteins tagged with green fluorescent protein. Importantly, we monitored the biosynthetic transport of these fusion proteins prior to their steady state distribution: transfected cells were incubated for 2 h at 37°C, then for 2 h at 20°C to accumulate fluorescent cargo in the trans-Golgi network (TGN) (pulse), followed by incubation at 37°C (chase) to allow budding of ISGs from the TGN (Kaether et al. 1997; Rudolf et al. 2001; Rudolf et al. 2003; Kogel et al. 2010). This permitted to monitor ISGs in an age- and thus maturation-dependent manner. The approach revealed unprecedented insights into the dynamics of ISGs in PC12 cells: (i) newly formed ISGs moved uni-directionally and in a microtubule-dependent manner to the cell periphery, (ii) ISGs were restricted to the F-actin rich cortex and underwent short directed movements while completing maturation such as the removal of furin (Rudolf et al. 2001, 2003), (iii) both the peripheral restriction and the movement of ISGs were found to be myosin Va-dependent, and (iv) the F-actin-dependent movement of ISGs decreased progressively during the ~3-h maturation period resulting in the immobilization of the major fraction of SGs (Rudolf et al. 2001). In agreement with this, we demonstrated by an in vitro binding assay that the fraction of fluorescent SGs, which detached from F-actin in an ATP-dependent manner, decreased progressively within the same time window (Kogel et al. 2010). Because the ATP-inhibited binding to F-actin in vitro could be mimicked by compromising myosin Va, a selective inactivation of ISG-associated myosin Va during maturation is conceivable (Kogel et al. 2010).
Role of Myosin Va in SG Maturation
What could be the underlying mechanism of the myosin Va-dependent membrane remodeling? First, given that SGs in PC12 cells extensively cluster in the case of myosin Va-tail fragment expression (Rudolf et al. 2003), similar to organelles of other cell types (Wu et al. 1998), sterical hindrance could impede membrane remodeling. However, this is unlikely because the block of furin removal was also evident upon siRNA silencing, which does not lead to clustering of SGs (Rudolf et al. 2003; Kogel et al. 2010). Second, in the light of our finding that active myosin Va is associated with ISGs (Kogel et al. 2010), it is tempting to speculate that this motor protein exerts a mechanical force to promote budding of IDV, similar to the role that has been proposed for myosin VI in the biogenesis of plasma membrane-derived clathrin-coated vesicles (Buss et al. 2001). Third, it is conceivable that myosin Va exerts an indirect role in membrane remodeling of ISGs such as the transport of SGs to a specific site or binding of an essential factor.
Role of Rab3D in SG Maturation
Because myosin Va does not directly interact with membranes (Miller and Sheetz 2000), Rab proteins as central mediators of membrane traffic are likely candidates to cooperate with myosin Va in the membrane remodeling of ISGs. In a systematic screen of Rab family members in PC12 cells only seven Rab isoforms (Rab3A/B/C/D, Rab27A/B, and Rab37) were specifically targeted to dense-core SGs (Fukuda 2008). Furthermore, intensive studies on Rab3 and Rab27 subfamily members clearly demonstrate an implication of both in regulated secretion, although the involvement of these proteins in specific steps of SG traffic remains unclear. In addition, recent observations indicate a role as sensor for the late maturation stages of SGs for Rab27 (Hannah et al. 2003; Merrins and Stuenkel 2008). Based on these data, we decided to analyze Rab3 isoforms as prime candidates for a potential role in SG maturation and found striking parallels to the role of myosin Va.
Because of our previous results indicating that ISGs complete their maturation in the F-actin rich cortex of PC12 cells, we first screened all four Rab3 isoforms and their nucleotide binding-deficient mutants for their potential interference with the cortical localization of ISGs. Transient expression of all constructs in PC12 cells, respectively, revealed a reduced cortical localization of SGs only in the case of the mutants of Rab3A and Rab3D. Subsequently, these two Rabs were analyzed for a potential role in membrane remodeling of maturing ISGs. Interestingly, only the expression of mutant Rab3D, but not of mutant Rab3A blocked the removal of furin (Fig. 1). Third, in contrast to myosin Va-tail expression, processing of the luminal SG protein secretogranin II was impeded upon expression of mutant Rab3D. The finding of clearly distinct functions of Rab3 isoforms on SG distribution and maturation was remarkable, because previous studies on the exocytosis of endocrine cells (Iezzi et al. 1999) and of mouse model neurons (Schluter et al. 2004) suggested that the four isoforms of Rab3 would play redundant roles.
Similar to Rab3D, the GGA3 clathrin adaptor protein and synaptotagmin IV were also found to be essential for both protein removal and prohormone processing. In the case of GGA3, its depletion not only led to a retention of syntaxin 6 and VAMP4 in MSGs but also to a decrease in activity of prohormone convertase 2 (Kakhlon et al. 2006). In the case of synaptotagmin IV, one of the SNAREs for homotypic SG fusion, this protein was shown to be necessary for cargo processing (Ahras et al. 2006). However, inhibition of ARF-1-recruitment to ISGs by brefeldin A blocked secretion from ISGs (Fernandez et al. 1997) and VAMP4 and synaptotagmin IV removal (Eaton et al. 2000), but not the processing of proopiomelanocortin (Fernandez et al. 1997). Thus, ARF-1 together with myosin Va (Kogel et al. 2010) represent examples of proteins executing a role in membrane remodeling of ISGs but not in cargo processing.
Our result of a specific role of Rab3D in SG maturation is in agreement with data obtained from several other studies. Indeed, Rab3D was found to associate with a population of SG with low buoyant density in parotid (Chan et al. 2000). Moreover, SGs of exocrine pancreas and parotid gland of Rab3D-knockout mice have an approximately doubled volume compared to wild-type littermates (Riedel et al. 2002). In addition, shrinkage of mouse zymogen granules at birth coincides with a first association of Rab3D with the zymogen granules (Ermak and Rothman 1983). Because these data indicate a role of Rab3D in determining the size of SGs, Riedel and colleagues speculated that Rab3D may restrict the homotypic fusion of ISGs (Riedel et al. 2002). However, we obtained in vitro evidence showing that neither wild type nor mutant Rab3D interfered with homotypic fusion of ISGs and thus argues against such a role of Rab3D. Instead, the demonstrated block of Rab3D mutant on furin removal suggests that the increased size of SGs in Rab 3D knockout mice could result from insufficient membrane removal during ISG maturation.
Cooperativity of Myosin Va and Rab3D?
Role of myosin Va and Rab3D in the life cycle of SGs
In general, the composition of complexes comprising Rab proteins, effectors, and myosin Va bound to SGs may change in a temporal fashion to regulate the sequential steps of SG biogenesis and trafficking, allowing elaborate fine-tuning of exocytosis in response to physiological conditions. In agreement with this hypothesis, many of the Rab and myosin binding proteins were shown to be embedded into higher regulatory networks involving protein kinase A/cAMP (Schluter et al. 1999; Kashima et al. 2001; Abderrahmani et al. 2006; Goehring et al. 2007), calcium-CaM-dependent kinase II (Schluter et al. 1999), micro RNA repressors, and transcription factors (Plaisance et al. 2006). Most relevant in light of our study is the recently demonstrated role of the transcriptional factor MIST1 in the down-regulation of Rab3D and in regulating exocrine granule maturation (Tian et al. 2010).
We thank Rüdiger Rudolf for comments on the manuscript.
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