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Cellular and Molecular Neurobiology

, Volume 31, Issue 1, pp 27–35 | Cite as

Dyslexia-Associated Kiaa0319-Like Protein Interacts with Axon Guidance Receptor Nogo Receptor 1

  • Ming-Wai Poon
  • Wan-Hong Tsang
  • Sun-On Chan
  • Hiu-Ming Li
  • Ho-Keung Ng
  • Mary Miu-Yee WayeEmail author
Original Research

Abstract

To identify the putative interacting partners for Kiaa0319-like protein. KIAA0319-like, located near the dyslexia susceptibility locus, DYX8 in chromosome 1p34.3, has been suggested as a positional candidate for developmental dyslexia due to its homology with another gene, KIAA0319 which has been strongly established as a candidate gene for developmental dyslexia. Previous research has shown that a single marker, rs7523017 (P = 0.042) has been associated with developmental dyslexia by a Canadian group. There is little functional information about this gene and protein. In this article, we put forward further evidence that support Kiaa0319-like is a candidate for this disorder. A yeast-2-hybrid screen and co-immunopreciptiation assays were performed to find protein interacting partners of KIAA0319L. A human cortex immunohistochemistry assay was performed to show the colocalization of Kiaa0319-like and its specific interacting partner in cells. Nogo Receptor 1 (NgR1), an axon guidance receptor, was identified to have physical interactions with Kiaa0319-like protein. These two proteins interact predominantly in the cytoplasmic granules of cortical neurons in the human brain cortex. Based on this data, it can be concluded that Kiaa0319-like protein interacts with Nogo Receptor 1, supporting the idea that Kiaa0319-like protein participates in axon guidance.

Keywords

Kiaa0319-like protein Developmental dyslexia Nogo Receptor 1 (NgR1 or RTN4R) Axon guidance Protein–protein interactions 

Notes

Acknowledgments

This study was partially supported by grants and a donation from the Croucher Foundation (to MMYW). MWP and WHT are the recipients of graduate studentships from the University Grants Council of Hong Kong.

Supplementary material

10571_2010_9549_MOESM1_ESM.ppt (3.6 mb)
Supplementary Fig. 1. Kiaa0319L protein did not self activate in the yeast two-hybrid system. Yeast strain AH109 was co-transformed with the bait plasmid, Kiaa0319L (N)-pGBK-BD and empty-pGAD-AD. No interaction was indicated by (A) β-galactosidase colorimetric filter assay and (B) SD/QDO culturing. (PPT 3647 kb)
10571_2010_9549_MOESM2_ESM.ppt (142 kb)
Supplementary Fig. 2. Schematic diagrams showing the predicted domains for human Kiaa0319 and Kiaa0319L proteins. Human Kiaa0319 protein sequence NP_055624.2 and human Kiaa0319L protein sequence NP_079150.3 are shown. (PPT 142 kb)

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Copyright information

© Springer Science+Business Media, LLC 2010

Authors and Affiliations

  • Ming-Wai Poon
    • 1
  • Wan-Hong Tsang
    • 1
  • Sun-On Chan
    • 1
  • Hiu-Ming Li
    • 2
  • Ho-Keung Ng
    • 2
  • Mary Miu-Yee Waye
    • 1
    Email author
  1. 1.School of Biomedical Sciences, Croucher Laboratory for Human GenomicsThe Chinese University of Hong KongShatin N.T., Hong KongChina
  2. 2.Department of Anatomical and Cellular PathologyThe Chinese University of Hong KongShatin, Hong KongChina

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