Spy1, as a member of the Speedy/RINGO family and a novel activator of cyclin-dependent kinases, was shown to promote cell cycle progression and cell survival in response to DNA damage. While its expression and roles in nervous system lesion and repair were still unknown. Here, we performed an acute sciatic nerve injury model in adult rats and studied the dynamic changes of Spy1 expression in lumbar spinal cord. Temporally, Spy1 expression was increased shortly after sciatic nerve crush and peaked at day 2. Spatially, Spy1 was widely expressed in the lumbar spinal cord including neurons and glial cells. While after injury, Spy1 expression was increased predominantly in astrocytes and microglia, which were largely proliferated. Moreover, there was a concomitant up-regulation of CDK2 activity and down-regulation of p27. Collectively, we hypothesized peripheral nerve injury induced an up-regulation of Spy1 in lumbar spinal cord, which was associated with glial proliferation.
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This study was supported by the National Natural Scientific Foundation of China Grant (No.30300099 and No.30770488), Natural Scientific Foundation of Jiangsu Province Grant (No.BK2003035 and No.BK2006547), College and University Natural Scientific Research Programme of Jiangsu Province (No.03KJB180109 and No. 04KJB320114), Technology Guidance Plan for Social Development of Jiangsu Province Grant (BS2004526), Health Project of Jiangsu Province (H200632), and “Liu-Da-Ren-Cai-Gao-Feng” Financial Assistance of Jiangsu Province Grant (No.2), Postgraduate Scientific Innovation Program of Jiangsu Province (No. CX08S_026Z).
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