Cellular and Molecular Neurobiology

, Volume 28, Issue 5, pp 737–744

Comparative Proteomics Analysis of Cerebrospinal Fluid of Patients with Guillain–Barré Syndrome

  • Yin-Rong Yang
  • Shi-Lian Liu
  • Zhao-Yu Qin
  • Fu-Jun Liu
  • Yan-Jiang Qin
  • Shu-Mei Bai
  • Zhe-Yu Chen
Original Paper

Abstract

To better understand the pathophysiologic mechanisms underlying Guillain–Barré syndrome (GBS), Comparative proteomic analysis of cerebrospinal fluid (CSF) between patients with GBS (the experiment group) and control subjects suffering from other neurological disorders (the control group) was carried out using two-dimensional gel electrophoresis (2-DE) technique, in combination with matrix-assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF MS) and database searching to determine abnormal CSF proteins in GBS patients. Image analysis of 2-DE gels silver stained revealed that 10 protein spots showed significant differential expression between the two groups of CSF samples. The expression of cystatin C, transthyretin, apolipoprotein E and heat shock protein 70 were decreased. However, haptoglobin, α-1-antitrypsin, apolipoprotein A-IV and neurofilaments were elevated. The subsequent ELISA measured the concentration of cystatin C and confirmed the result of the proteomic analysis. These identified proteins may be involved in the pathophysiological process of GBS and call for further studying the role of these proteins in the pathogenesis of the disease.

Keywords

Cerebrospinal fluid Guillain–Barré syndrome Proteome Two-dimensional electrophoresis Mass Spectrometry ELISA 

Copyright information

© Springer Science+Business Media, LLC 2008

Authors and Affiliations

  • Yin-Rong Yang
    • 1
  • Shi-Lian Liu
    • 1
  • Zhao-Yu Qin
    • 2
  • Fu-Jun Liu
    • 3
  • Yan-Jiang Qin
    • 4
  • Shu-Mei Bai
    • 1
  • Zhe-Yu Chen
    • 5
  1. 1.Institute of Biochemistry and Molecular Biology, School of MedicineShandong UniversityJinanP.R. China
  2. 2.Biology Science Department in Marine CollegeShandong University at WeihaiWeihaiChina
  3. 3.Central LaboratoryYuhuangding Hospital of Qingdao UniversityYantaiChina
  4. 4.Department of NeurologyQilu Hospital of Shandong UniversityJinanChina
  5. 5.Institute of Neurobiology, School of MedicineShandong UniversityJinanChina

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