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Cellular and Molecular Neurobiology

, Volume 26, Issue 7–8, pp 1325–1340 | Cite as

The Effect of Endurance Training on Regional Serotonin Metabolism in the Brain During Early Stage of Detraining Period in the Female Rat

  • Józef LangfortEmail author
  • Elzbieta Barańczuk
  • Dariusz Pawlak
  • Małgorzata Chalimoniuk
  • Nadežda Lukačova
  • Jozef Maršala
  • Jan Górski
Article

1. The effect was examined of a single bout of nonexhaustive endurance exercise on tryptophan (Try), serotonin (5-HT), 5-hydroxyindolacetic acid (5-HIAA), and tryptophan hydroxylase (TpH) levels in different parts of rat brain (brain cortex, cerebellum, hypothalamus, midbrain striatum, medulla) on the last day of endurance training and 48 h later (detraining period).

2. Female rats were subjected to a 6-week endurance training programme. The effectiveness of the training was evaluated by measuring anaerobic threshold (AT). High performance liquid chromatography (HPLC) was used to determine regional Try, 5-HT, and 5-HIAA contents in the brain, and thin layer chromatography followed by gas-liquid chromatography was used to determine blood levels of free fatty acids. Regional TpH levels were measured by Western blot analysis.

3. In the two rat groups subjected to endurance training, in all brain regions studied but cerebellum, 5-HT content was significantly lower after the last bout of nonexhaustive endurance exercise than in resting control rats that were not subjected to the training. Similarly, the cortical and striatal, but not cerebellar, 5-HT/Try ratios were significantly lower in the trained rats at the end of the last training session and at the end of a single bout of nonexhaustive exercise administered after a 48-h detraining period than in the controls. TpH protein level was decreased by 15–25% after the last bout of exercise either during the training process or after the and 1 h bout of endurance exercise performed 48 h after cessation of endurance training in brain cortex and striatum but not cerebellar.

4. These results indicate that the reduction in 5-HT level was the adaptive response to endurance training. The lowered 5-HT/Try ratio and lowered TpH protein level attained after the training process suggests and that this change may be, at least partially, attributed to downregulation of TpH activity.

KEY WORDS:

endurance training free tryptophan serotonin 5-hydroxyindolacetic acid brain cortex cerebellum hypothalamus midbrain striatum medulla 

Abbreviations used

AT

anaerobic threshold

BCAA

branched-chain amino acids

Km

Michaelis–Menten constant

EDTA

ethylenediaminetetraacetic acid (sodium salt)

FFA

free fatty acids

f-Try

free l-tryptophan

HPLC

high performance liquid chromatography

5-HIAA

5-hydroxyindolacetic acid

LA

lactate

Vmax

maximal enzyme activity

5-HT

serotonin

5-HT1BR

serotonin 1B receptor

Try

l-tryptophan

TpH

tryptophan hydroxylase

Notes

ACKNOWLEDGMENTS

Dr S. J. Chrapusta of the Department of Experimental Pharmacology, PAS, Medical Research Center, is gratefully acknowledged for his critical reading of the manuscript. This paper was made possible with support from the Medical University of Bialystok (Grant no 3-18 980 and 4-18 493) and by the VEGA grants No. 2/5134/25 and 2/3217/23 from the SAS, APVT. Grant No. 51-013002 and PAS/SAS project 17/24-26, and PAS/SAS project 17/24-26.

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Copyright information

© Springer Science+Business Media, Inc. 2006

Authors and Affiliations

  • Józef Langfort
    • 1
    • 6
    Email author
  • Elzbieta Barańczuk
    • 2
  • Dariusz Pawlak
    • 3
  • Małgorzata Chalimoniuk
    • 4
  • Nadežda Lukačova
    • 5
  • Jozef Maršala
    • 5
  • Jan Górski
    • 2
  1. 1.Department of Experimental Pharmacology, Medical Research CenterPolish Academy of SciencesWarsawPoland
  2. 2.Department of PhysiologyMedical University of BiałystokBiałystokPoland
  3. 3.Department of PharmacodynamicsMedical Universy of BiałystokBiałystokPoland
  4. 4.Department of Cellular Signaling, Medical Research CenterPolish Academy of SciencesWarsawPoland
  5. 5.Institute of NeurobiologySlovak Academy of SciencesKošiceSlovak Republic
  6. 6.Department of Experimental Pharmacology, Medical Research CenterPolish Academy of SciencesWarsawPoland

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