Cellular and Molecular Neurobiology

, Volume 26, Issue 2, pp 109–118 | Cite as

Adrenomedullin Improves the Blood–Brain Barrier Function Through the Expression of Claudin-5

  • Masaru HondaEmail author
  • Shinsuke Nakagawa
  • Kentaro Hayashi
  • Naoki Kitagawa
  • Keisuke Tsutsumi
  • Izumi Nagata
  • Masami Niwa


1. Aims: Brain vascular endothelial cells secret Adrenomedullin (AM) has multifunctional biological properties. AM affects cerebral blood flow and blood–brain barrier (BBB) function. We studied the role of AM on the permeability and tight junction proteins of brain microvascular endothelial cells (BMEC).

2. Methods: BMEC were isolated from rats and a BBB in vitro model was generated. The barrier functions were studied by measuring the transendothelial electrical resistance (TEER) and the permeability of sodium fluorescein and Evans’ blue albumin. The expressions of tight junction proteins were analyzed using immunocytochemistry and immunoblotting.

3. Results: AM increased TEER of BMEC monolayer dose-dependently. Immunocytochemistry revealed that AM enhanced the claudin-5 expression at a cell–cell contact site in a dose-dependent manner. Immunoblotting also showed an overexpression of claudin-5 in AM exposure.

4.Conclusions: AM therefore inhibits the paracellular transport in a BBB in vitro model through claudin-5 overexpression.

Key Words

adrenomedullin blood–brain barrier claudin-5 


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Copyright information

© Springer Science+Business Media, Inc. 2006

Authors and Affiliations

  • Masaru Honda
    • 1
    • 3
    Email author
  • Shinsuke Nakagawa
    • 2
  • Kentaro Hayashi
    • 1
  • Naoki Kitagawa
    • 1
  • Keisuke Tsutsumi
    • 1
  • Izumi Nagata
    • 1
  • Masami Niwa
    • 2
  1. 1.Department of NeurosurgeryNagasaki University School of MedicineNagasakiJapan
  2. 2.Department of Pharmacology 1Nagasaki University School of MedicineNagasakiJapan
  3. 3.NagasakiJapan

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