pp 1–13 | Cite as

Enhanced production of bacterial cellulose by Komactobacter intermedius using statistical modeling

  • Shella Permatasari Santoso
  • Chih-Chan Chou
  • Shin-Ping Lin
  • Felycia Edi Soetaredjo
  • Suryadi Ismadji
  • Chang-Wei Hsieh
  • Kuan Chen ChengEmail author
Original Research


The extraordinary nature of the bacterial cellulose (BC) biopolymer gives it potential for diverse applications; however, the low BC yield of many indigenous cellulose-producing bacteria is a persistent problem in its synthesis. In this study, the BC yield of Komactobacter intermedius (BCRC 910677) was optimized by modifying culture media. The optimal culture period, type of carbon, and nitrogen sources were evaluated using the one-factor-at-a-time approach prior to the optimization study. The optimization was done by using the response surface methodology (RSM). In RSM optimization study, a Box–Behnken design with three parameters is applied; the three parameters include fructose concentrations (X1), peptone concentrations (X2), and pH values (X3). Our optimal culture media combined 41 g/L of fructose, 38 g/L peptone, and a pH of 5.2. The predicted BC yield from the RSM model is 4.012 g/L, while BC yield of 3.906 g/L is obtained from the experiment using the optimized medium; that is only 2.64% difference. An increase in BC production of 3.82-fold (compared to the culture in HS medium) was obtained after 6-days culture. The K. intermedius investigated in this study show great potential for commercial BC productions and as feedstock. The RSM can be a promising approach to enhance BC yield since the parameters were well correlated.


Bacterial cellulose Komagataeibacter Komactobacter Response surface methodology Nata de coco 



This study was partly funded by the Ministry of Science and Technology, Taiwan, under Contract No. 106-2628-E-002-009-MY3.

Compliance with ethical standards

Conflict interest

The authors declare that they have no conflict of interest.

Supplementary material

10570_2019_2961_MOESM1_ESM.docx (263 kb)
Supplementary material 1 (DOCX 262 kb)


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Copyright information

© Springer Nature B.V. 2020

Authors and Affiliations

  1. 1.Department of Chemical EngineeringWidya Mandala Surabaya Catholic UniversitySurabayaIndonesia
  2. 2.Department of Chemical EngineeringNational Taiwan University of Science and TechnologyTaipeiTaiwan
  3. 3.Institute of BiotechnologyNational Taiwan UniversityTaipeiTaiwan
  4. 4.Department of Food SafetyTaipei Medical UniversityTaipeiTaiwan
  5. 5.Department of Food Science and BiotechnologyNational Chung Hsing UniversityTaichungTaiwan
  6. 6.Graduate Institute of Food Science and TechnologyNational Taiwan UniversityTaipeiTaiwan
  7. 7.Department of Medical Research, China Medical University HospitalChina Medical UniversityTaichungTaiwan

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