, Volume 27, Issue 1, pp 369–383 | Cite as

Facile synthesis of bacterial cellulose and polyethyleneimine based hybrid hydrogels for antibacterial applications

  • Fazli Wahid
  • He Bai
  • Feng-Ping Wang
  • Yan-Yan Xie
  • Yan-Wen Zhang
  • Li-Qiang Chu
  • Shi-Ru Jia
  • Cheng ZhongEmail author
Original Research


Bacterial cellulose (BC) has numerous unique properties; however, the lack of antibacterial properties has restricted its biomedical applications. To resolve this problem, we have developed BC/polyethyleneimine (PEI)-based antibacterial hydrogels by a facile two-step-one-pot method using epichlorohydrin (ECH) as a coupling agent. ECH was added to BC solution and homogenized, followed by cross-linking with different content of PEI. The prepared hydrogels showed thermal stability, moldability, and PEI-content dependent mechanical properties. Furthermore, the antibacterial properties of the prepared hydrogels were investigated against S. aureus and E. coli by agar well diffusion method and colony forming unit method. All hydrogels containing PEI showed antibacterial properties dependent on PEI content. The hydrogels containing PEI ≥ 12.88 mg/mL showed more than 99% antibacterial activity against both strains. We believe that the prepared hydrogels can find potential applications in biomedical fields.

Graphic abstract


Bacterial cellulose Polyethyleneimine Hydrogels Antibacterial activity 



The authors are grateful for the financial support from the National Natural Science Foundation of China (Nos. 21978219, 21576212), Natural Science Foundation of Tianjin (Nos. 17YFZCSF01120, 18PTSYJC00140).

Supplementary material

10570_2019_2806_MOESM1_ESM.docx (2 mb)
Supplementary material 1 (DOCX 2036 kb)


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Copyright information

© Springer Nature B.V. 2019

Authors and Affiliations

  1. 1.State Key Laboratory of Food Nutrition and SafetyTianjin University of Science and TechnologyTianjinPeople’s Republic of China
  2. 2.Key Laboratory of Industrial Fermentation Microbiology, (Ministry of Education)Tianjin University of Science and TechnologyTianjinPeople’s Republic of China
  3. 3.College of Chemical Engineering and Materials ScienceTianjin University of Science and TechnologyTianjinPeople’s Republic of China
  4. 4.China Offshore Environmental Service Ltd.TianjinPeople’s Republic of China

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