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Improvement of platelet aggregation and rapid induction of hemostasis in chitosan dressing using silver nanoparticles

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Abstract

There is an increasing demand for high-performance hemostatic agents with good antibacterial efficiency and biocompatibility. In this study, a chitosan/gelatin/sodium hyaluronate hemostatic dressing with silver nanoparticles (CGSH/Ag50) was designed and characterized. Our results suggest that CGSH/Ag50 has high blood absorption and promotes platelet aggregation by the activation of positively charged surface and stimulation of silver nanoparticles. Owing to these excellent properties, CGSH/Ag50 could rapidly control hemorrhage in rabbit ear and liver injury models. CGSH/Ag50 also shows good antibacterial activity, good liquid absorption, and significantly promotes full-thickness wound healing. In the hemolysis and L929 cell cytotoxicity assays, CGSH/Ag50 exhibited no cytotoxicity, suggesting good hemocompatibility and biocompatibility. These observations suggest that CGSH/Ag50 can be an efficient hemostatic material with enough biocompatibility to be used for controlling hemorrhage and promoting wound healing after hemostasis surgery.

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Acknowledgments

This work was supported by the National Natural Science Foundation of China (No. 51703185) and the Key Research and Development Program (Social Development) of Zhenjiang City (No. SH2018001).

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Correspondence to Guangqian Lan.

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Lan, G., Li, Q., Lu, F. et al. Improvement of platelet aggregation and rapid induction of hemostasis in chitosan dressing using silver nanoparticles. Cellulose 27, 385–400 (2020) doi:10.1007/s10570-019-02795-1

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Keywords

  • Hemostasis
  • Silver nanoparticles
  • Wound healing
  • Antibacterial activity
  • Chitosan