, Volume 27, Issue 1, pp 335–345 | Cite as

Regenerated cellulose-based composite membranes as adsorbent for protein adsorption

  • Qi Zhou
  • Yuping Bao
  • Hao Zhang
  • Qian Luan
  • Hu TangEmail author
  • Xiuting LiEmail author
Original Research


The development of protein adsorbents with high adsorption performance has attracted great attention due to the important role of these adsorbents in protein separation and purification. Herein, cellulose-based composite membranes were prepared through a combination of cellulose fiber (CF) and 2,2,6,6-tetramethylpiperidine-1-oxyl oxidized cellulose nanofiber (CNF) in alkaline/urea aqueous solution and regeneration from a tape casting method. Taking advantage of the functional carboxyl groups in CNF, the obtained cellulose-based composite membranes display a good protein adsorption property, with a capacity of 241.6 mg g−1, by using bovine serum albumin as a model protein. Meanwhile, the adsorption performance of cellulose-based composite membranes can be optimized by regulating the blending ratios of CNF and CF, buffer pH, initial protein concentrations and temperature, and the mechanical property of cellulose-based composite membranes can be adjusted by changing the ratio of CNF and CF. These effective and economic membranes may act as promising candidates for protein adsorption in protein separation and purification fields.


Cellulose nanofiber Cellulose fiber Membrane Protein adsorption 



This work was supported by the Open Project from Beijing Advanced Innovation Center for Food Nutrition and Human Health, Beijing Technology and Business University (BTBU) (No. 20182013).

Supplementary material

10570_2019_2761_MOESM1_ESM.docx (232 kb)
Supplementary material 1 (DOCX 231 kb)


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Copyright information

© Springer Nature B.V. 2019

Authors and Affiliations

  1. 1.Beijing Advanced Innovation Center for Food Nutrition and Human HealthBeijing Technology and Business UniversityBeijingChina
  2. 2.Key Laboratory of Oilseeds Processing, Ministry of Agriculture, Hubei Key Laboratory of Lipid Chemistry and Nutrition, Oil Crops and Lipids Process Technology National and Local Joint Engineering LaboratoryOil Crops Research Institute, Chinese Academy of Agricultural SciencesWuhanChina

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