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pp 1–13 | Cite as

Sensitivity enhancement of lateral flow assay by embedding cotton threads in paper

  • Su-Feng Zhang
  • Li-Na Liu
  • Rui-Hua TangEmail author
  • Zhi Liu
  • Xiao-Cong He
  • Zhi-Guo Qu
  • Fei LiEmail author
Original Research
  • 24 Downloads

Abstract

Lateral flow assays (LFAs) have been extensively used as point-of-care testing platforms because they are inexpensive, portable, simple, and rapid, which particularly improve their availability in resource-poor settings. However, the poor sensitivity of LFAs restricts their further applications. Herein, we proposed a novel and simple method to enhance the detection sensitivity of LFAs by embedding cotton thread-based barriers into paper and further integrating them into strips to decrease the flow rate of sample and extend the reaction time. The number and hydrophilicity of the embedded cotton threads were sequentially optimized. The flow rate of liquid in cotton thread-embedded LFAs was mathematically simulated using a circuit-like model and the simulation results are consistent with the experimental results. With using human immunodeficiency virus nucleic acid as a model target, the cotton thread-embedded LFAs presented a fourfold enhancement in detection sensitivity compared to that of the unmodified LFAs. The strategy of embedding cotton threads into paper possesses great potential for fabricating other paper-based microfluidic devices in the future.

Graphic abstract

Keywords

Lateral flow assays (LFAs) Hydrophobic barrier Cotton threads Detection sensitivity Flow rate 

Notes

Acknowledgments

This work was financially supported by the Key scientific research plan (Key Laboratory) of Shaanxi Provincial Education Department (17JS016), International Joint Research Center for Biomass Chemistry and Materials, Shaanxi International Science and Technology Cooperation Base (2018GHJD-19), the National Natural Science Foundation of China (21808132), the General Financial Grant from the China Postdoctoral Science Foundation (2016M592773, 2018M633525), the Natural Science Research Foundation of Shaanxi University of Science & Technology (2017BJ-35), the Project of Shaanxi Provincial Education Department (18JK0096) and the Key Program for Science and Technology Innovative Research Team in Shaanxi Province of China (2017KCT-22).

Supplementary material

10570_2019_2677_MOESM1_ESM.docx (2.7 mb)
Supplementary material 1 (DOCX 2743 kb)

References

  1. Abbas R, Khereby MA, Sadik WA, El Demerdash AGM (2014) Fabrication of durable and cost effective superhydrophobic cotton textiles via simple one step process. Cellulose 22:887–896.  https://doi.org/10.1007/s10570-014-0514-x CrossRefGoogle Scholar
  2. Adhikari M et al (2015) Aptamer-phage reporters for ultrasensitive lateral flow assays. Anal Chem 87:11660–11665.  https://doi.org/10.1021/acs.analchem.5b00702 CrossRefGoogle Scholar
  3. Banerjee SS, Roychowdhury A, Taneja N, Janrao R, Khandare J, Paul D (2013) Chemical synthesis and sensing in inexpensive thread-based microdevices. Sens Actuators B Chem 186:439–445.  https://doi.org/10.1016/j.snb.2013.06.036 CrossRefGoogle Scholar
  4. Burd EM (2010) Validation of laboratory-developed molecular assays for infectious diseases. Clin Microbiol Rev 23:550–576.  https://doi.org/10.1128/CMR.00074-09 CrossRefGoogle Scholar
  5. Caschera D et al (2013) Effects of plasma treatments for improving extreme wettability behavior of cotton fabrics. Cellulose 21:741–756.  https://doi.org/10.1007/s10570-013-0123-0 CrossRefGoogle Scholar
  6. Cate DM, Adkins JA, Mettakoonpitak J, Henry CS (2015) Recent developments in paper-based microfluidic devices. Anal Chem 87:19–41.  https://doi.org/10.1021/ac503968p CrossRefGoogle Scholar
  7. Cho IH, Bhunia A, Irudayaraj J (2015) Rapid pathogen detection by lateral-flow immunochromatographic assay with gold nanoparticle-assisted enzyme signal amplification. Int J Food Microbiol 206:60–66.  https://doi.org/10.1016/j.ijfoodmicro.2015.04.032 CrossRefGoogle Scholar
  8. Choi DH et al (2010) A dual gold nanoparticle conjugate-based lateral flow assay (LFA) method for the analysis of troponin I. Biosens Bioelectron 25:1999–2002.  https://doi.org/10.1016/j.bios.2010.01.019 CrossRefGoogle Scholar
  9. Choi JR et al (2016) Polydimethylsiloxane-paper hybrid lateral flow assay for highly sensitive point-of-care nucleic acid testing. Anal Chem 88:6254–6264.  https://doi.org/10.1021/acs.analchem.6b00195 CrossRefGoogle Scholar
  10. Choi JR et al (2017) Lateral flow assay based on paper-hydrogel hybrid material for sensitive point-of-care detection of dengue virus. Adv Healthc Mater 6:1600920.  https://doi.org/10.1002/adhm.201600920 CrossRefGoogle Scholar
  11. Choi JR, Nilghaz A, Chen L, Chou KC, Lu X (2018) Modification of thread-based microfluidic device with polysiloxanes for the development of a sensitive and selective immunoassay. Sens Actuators B Chem 260:1043–1051.  https://doi.org/10.1016/j.snb.2018.01.102 CrossRefGoogle Scholar
  12. de Puig H, Bosch I, Gehrke L, Hamad-Schifferli K (2017) Challenges of the nano-bio interface in lateral flow and dipstick immunoassays. Trends Biotechnol 35:1169–1180.  https://doi.org/10.1016/j.tibtech.2017.09.001 CrossRefGoogle Scholar
  13. Deng X, Wang C, Gao Y, Li J, Wen W, Zhang X, Wang S (2018) Applying strand displacement amplification to quantum dots-based fluorescent lateral flow assay strips for HIV-DNA detection. Biosens Bioelectron 105:211–217.  https://doi.org/10.1016/j.bios.2018.01.039 CrossRefGoogle Scholar
  14. Erenas MM, Orbe-Payá Id, Capitan-Vallvey LF (2016) Surface modified thread-based microfluidic analytical device for selective potassium analysis. Anal Chem 88:5331–5337CrossRefGoogle Scholar
  15. Gao Y, Deng X, Wen W, Zhang X, Wang S (2017) Ultrasensitive paper based nucleic acid detection realized by three-dimensional DNA-AuNPs network amplification. Biosens Bioelectron 92:529–535.  https://doi.org/10.1016/j.bios.2016.10.068 CrossRefGoogle Scholar
  16. He Y et al (2011) Ultrasensitive nucleic acid biosensor based on enzyme-gold nanoparticle dual label and lateral flow strip biosensor. Biosens Bioelectron 26:2018–2024.  https://doi.org/10.1016/j.bios.2010.08.079 CrossRefGoogle Scholar
  17. Hong S, Kwak R, Kim W (2016) Paper-based flow fractionation system applicable to preconcentration and field-flow separation. Anal Chem 88:1682–1687.  https://doi.org/10.1021/acs.analchem.5b03682 CrossRefGoogle Scholar
  18. Hossain SM, Brennan JD (2011) β-Galactosidase-based colorimetric paper sensor for determination of heavy metals. Anal Chem 83:8772–8778.  https://doi.org/10.1021/ac202290d CrossRefGoogle Scholar
  19. Hossain SMZ, Luckham RE, McFadden MJ, Brennan John D (2009) Reagentless bidirectional lateral flow bioactive paper sensors for detection of pesticides in beverage and food samples. Anal Chem 81:9055–9064CrossRefGoogle Scholar
  20. Hu J, Wang L, Li F, Han YL, Lin M, Lu TJ, Xu F (2013) Oligonucleotide-linked gold nanoparticle aggregates for enhanced sensitivity in lateral flow assays. Lab Chip 13:4352–4357.  https://doi.org/10.1039/c3lc50672j CrossRefGoogle Scholar
  21. Hu J, Wang S, Wang L, Li F, Pingguan-Murphy B, Lu TJ, Xu F (2014) Advances in paper-based point-of-care diagnostics. Biosens Bioelectron 54:585–597.  https://doi.org/10.1016/j.bios.2013.10.075 CrossRefGoogle Scholar
  22. Hu J et al (2016) Sensitive and quantitative detection of c-reaction protein based on immunofluorescent nanospheres coupled with lateral flow test strip. Anal Chem 88:6577–6584.  https://doi.org/10.1021/acs.analchem.6b01427 CrossRefGoogle Scholar
  23. Lee JH et al (2015) Multiplex diagnosis of viral infectious diseases (AIDS, hepatitis C, and hepatitis A) based on point of care lateral flow assay using engineered proteinticles. Biosens Bioelectron 69:213–225.  https://doi.org/10.1016/j.bios.2015.02.033 CrossRefGoogle Scholar
  24. Lee D, Shin Y, Chung S, Hwang KS, Yoon DS, Lee JH (2016) Simple and highly sensitive molecular diagnosis of Zika virus by lateral flow assays. Anal Chem 88:12272–12278.  https://doi.org/10.1021/acs.analchem.6b03460 CrossRefGoogle Scholar
  25. Li X, Tian J, Shen W (2010) Thread as a versatile material for low-cost microfluidic diagnostics. ACS Appl Mater Interfaces 2:1–6.  https://doi.org/10.1021/am9006148 CrossRefGoogle Scholar
  26. Li YD, Li WY, Chai HH, Fang C, Kang YJ, Li CM, Yu L (2018) Chitosan functionalization to prolong stable hydrophilicity of cotton thread for thread-based analytical device application. Cellulose 25:4831–4840.  https://doi.org/10.1007/s10570-018-1891-3 CrossRefGoogle Scholar
  27. Liu Y et al (2015) Detection of 3-phenoxybenzoic acid in river water with a colloidal gold-based lateral flow immunoassay. Anal Biochem 483:7–11.  https://doi.org/10.1016/j.ab.2015.04.022 CrossRefGoogle Scholar
  28. Liu X et al (2018) Multiple SNPs detection based on lateral flow assay for phenylketonuria diagnostic. Anal Chem 90:3430–3436.  https://doi.org/10.1021/acs.analchem.7b05113 CrossRefGoogle Scholar
  29. Malon RSP, Heng LY, Córcoles EP (2017) Recent developments in microfluidic paper-, cloth-, and thread-based electrochemical devices for analytical chemistry. Rev Anal Chem.  https://doi.org/10.1515/revac-2016-0018 Google Scholar
  30. Nilghaz A, Ballerini DR, Shen W (2013) Exploration of microfluidic devices based on multi-filament threads and textiles: a review. Biomicrofluidics 7:51501.  https://doi.org/10.1063/1.4820413 CrossRefGoogle Scholar
  31. Nilghaz A, Ballerini DR, Fang X-Y, Shen W (2014) Semiquantitative analysis on microfluidic thread-based analytical devices by ruler. Sens Actuators B Chem 191:586–594.  https://doi.org/10.1016/j.snb.2013.10.023 CrossRefGoogle Scholar
  32. Parolo C, Medina-Sanchez M, de la Escosura-Muniz A, Merkoci A (2013) Simple paper architecture modifications lead to enhanced sensitivity in nanoparticle based lateral flow immunoassays. Lab Chip 13:386–390.  https://doi.org/10.1039/c2lc41144j CrossRefGoogle Scholar
  33. Pipatchanchai T, Srikulkit K (2007) Hydrophobicity modification of woven cotton fabric by hydrophobic fumed silica coating. J Sol-Gel Sci Technol 44:119–123.  https://doi.org/10.1007/s10971-007-1609-8 CrossRefGoogle Scholar
  34. Raeisossadati MJ, Danesh NM, Borna F, Gholamzad M, Ramezani M, Abnous K, Taghdisi SM (2016) Lateral flow based immunobiosensors for detection of food contaminants. Biosens Bioelectron 86:235–246.  https://doi.org/10.1016/j.bios.2016.06.061 CrossRefGoogle Scholar
  35. Reches M, Mirica KA, Dasgupta R, Dickey MD, Butte MJ, Whitesides GM (2010) Thread as a matrix for biomedical assays. ACS Appl Mater Interfaces 2:1722–1728.  https://doi.org/10.1021/am1002266 CrossRefGoogle Scholar
  36. Rivas L, Medina-Sanchez M, de la Escosura-Muniz A, Merkoci A (2014) Improving sensitivity of gold nanoparticle-based lateral flow assays by using wax-printed pillars as delay barriers of microfluidics. Lab Chip 14:4406–4414.  https://doi.org/10.1039/c4lc00972j CrossRefGoogle Scholar
  37. Songok J, Toivakka M (2016) Controlling capillary-driven surface flow on a paper-based microfluidic channel. Microfluid Nanofluid 20:9.  https://doi.org/10.1007/s10404-016-1726-1 CrossRefGoogle Scholar
  38. Tang R et al (2016) Improved sensitivity of lateral flow assay using paper-based sample concentration technique. Talanta 152:269–276.  https://doi.org/10.1016/j.talanta.2016.02.017 CrossRefGoogle Scholar
  39. Terao Y, Yonekita T, Morishita N, Fujimura T, Matsumoto T, Morimatsu F (2013) Potential rapid and simple lateral flow assay for Escherichia coli O111. J Food Prot 76:755–761.  https://doi.org/10.4315/0362-028X.JFP-12-351 CrossRefGoogle Scholar
  40. Toley BJ, McKenzie B, Liang T, Buser JR, Yager P, Fu E (2013) Tunable-delay shunts for paper microfluidic devices. Anal Chem 85:11545–11552.  https://doi.org/10.1021/ac4030939 CrossRefGoogle Scholar
  41. Wallis RS, Pai M, Menzies D, Doherty TM, Walzl G, Perkins MD, Zumla A (2010) Biomarkers and diagnostics for tuberculosis: progress, needs, and translation into practice. Lancet 375:1920–1937.  https://doi.org/10.1016/S01406736(10)60359-5 CrossRefGoogle Scholar
  42. Wang R et al (2018) Highly sensitive detection of high-risk bacterial pathogens using SERS-based lateral flow assay strips. Sens Actuators B Chem 270:72–79.  https://doi.org/10.1016/j.snb.2018.04.162 CrossRefGoogle Scholar
  43. Wong SY, Cabodi M, Rolland J, Klapperich CM (2014) Evaporative concentration on a paper-based device to concentrate analytes in a biological fluid. Anal Chem 86:11981–11985.  https://doi.org/10.1021/ac503751a CrossRefGoogle Scholar
  44. Zhou G, Mao X, Juncker D (2012) Immunochromatographic assay on thread. Anal Chem 84:7736–7743.  https://doi.org/10.1021/ac301082d CrossRefGoogle Scholar

Copyright information

© Springer Nature B.V. 2019

Authors and Affiliations

  1. 1.Shaanxi Provincial Key Laboratory of Papermaking Technology and Specialty Paper Development, National Demonstration Center for Experimental Light Chemistry Engineering EducationShaanxi University of Science and TechnologyXi’anPeople’s Republic of China
  2. 2.Bioinspired Engineering and Biomechanics Center (BEBC)Xi’an Jiaotong UniversityXi’anPeople’s Republic of China
  3. 3.The Key Laboratory of Biomedical Information Engineering of Ministry of Education, School of Life Science and TechnologyXi’an Jiaotong UniversityXi’anPeople’s Republic of China
  4. 4.Key Laboratory of Thermo-Fluid Science and Engineering of Ministry of Education, School of Energy and Power EngineeringXi’an Jiaotong UniversityXi’anPeople’s Republic of China

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