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Consumption of sugars and inhibitors of softwood hemicellulose hydrolysates as carbon sources for polyhydroxybutyrate (PHB) production with Paraburkholderia sacchari IPT 101

  • Karolin Dietrich
  • Marie-Josée DumontEmail author
  • Valérie Orsat
  • Luis F. Del Rio
Original Research
  • 38 Downloads

Abstract

The future industrial success of the compostable bio-polyesters known as polyhydroxyalkanoates, which includes polyhydroxybutyrate (PHB), depends mainly on their production using cheaper carbon sources than food-derived glucose. The existing pulp and paper infrastructure enables an alternative sugar supply in the form of wood hydrolysates. Softwood hemicellulose hydrolysates have a favourable sugar profile for fermentations and can be produced in such emerging integrated forest biorefineries. The processes can lead to varying amounts of inhibitors which can lead to the reduction or prevention of bacterial growth. A dilute acid pretreatment was used to produce a softwood hemicellulose hydrolysate containing the sugars mannose, xylose, glucose, galactose, and arabinose. To study the effects of increasing sugar diversity and inhibitor concentration, the softwood hydrolysate was mixed in varying proportions with a hardwood holocellulose hydrolysate obtained from the TMP-Bio process (a thermomechanical pulping-based process producing glucose and xylose). In fermentations with Paraburkholderia sacchari IPT 101, the sugars were depleted at different rates in the following order: glucose, mannose, xylose, galactose, and arabinose. All potential inhibitors except phenols were metabolized. The maximum cell dry weight reached 6.7 ± 0.1 g/L with 71 ± 5% PHB with hardwood holocellulose hydrolysate after 48 h. The analyses of the sugar and inhibitor consumption provided valuable information to validate approaches for the detoxification of softwood hemicellulose hydrolysates. Overall, the detoxification would allow PHB production in an integrated softwood biorefinery scheme.

Keywords

Softwood Hardwood Inhibitors PHA Biorefinery 

Abbreviations

ANOVA

Analysis of variance

CDM

Cell dry mass

HHH

Hardwood holocellulose hydrolysate from thermomechanical pulping

HMF

5-Hydroxymethylfurfural

IC

Ion chromatography

n.d.

Not determined

OD

Optical density

PHA

Polyhydroxyalkanoate

PHB

Poly[(R)-3-hydroxybutyrate]

PHB4HB

Poly[(R)-3-hydroxybutyrate-co-4-hydroxybutyrate]

PHBHHx

Poly[(R)-3-hydroxybutyrate-co-(R)-3-hydroxyhexanoate]

PHBV

Poly[(R)-3-hydroxybutyrate-co-(R)-3-hydroxyvalerate]

PHBVP

Poly((R)-3-hydroxybutyrate-co-(R)-3-hydroxyvalerate-co-3-hydroxypropionate)

SHH

Softwood hemicellulose hydrolysate from dilute acid hydrolysis

VFA

Volatile fatty acids

Notes

Acknowledgments

The financial support from the Richard H. Tomlinson Doctoral Fellowship and the National Science and Engineering Research Council of Canada (NSERC) is gratefully acknowledged.

Compliance of ethical standards

Conflict of interest

The authors declare that they have no competing interests.

Supplementary material

10570_2019_2664_MOESM1_ESM.pdf (669 kb)
Supplementary material 1 (PDF 668 kb)

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Copyright information

© Springer Nature B.V. 2019

Authors and Affiliations

  1. 1.Bioresource Engineering DepartmentMcGill UniversitySte-Anne de BellevueCanada
  2. 2.FPInnovationsPointe-ClaireCanada

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