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Cellulose

, Volume 26, Issue 7, pp 4279–4290 | Cite as

Enzymatic production of cello-oligomers with endoglucanases

  • Stefanie Kluge
  • Benjamin Bonhage
  • Jörn Viell
  • Mari Granström
  • Alois Kindler
  • Antje C. SpiessEmail author
Original Research
  • 65 Downloads

Abstract

Cello-oligomers have a wide application range and are gained from three different cello-oligomer production paths, which show different drawbacks, namely acid or alkaline hydrolysis of cellulose, chemical or enzymatic synthesis, and enzymatic hydrolysis of cellulose using cellulase mixtures. Therefore, this study aims at a novel route towards cello-oligomers using purified endoglucanases. From the renewable raw material cellulose, pretreated by an ionic liquid, cello-oligomers with a weight average degree of polymerization (DPW) of 65 were directly obtained by enzymatic hydrolysis. During hydrolysis, between 14.1 and 24.5 mass percent of monomeric and dimeric sugars were formed as byproduct. A second ionic liquid pretreatment of the cellulose, remaining after the first hydrolysis, and a second enzymatic hydrolysis resulted in cello-oligomers with a DP of 35. XRD deconvolution and Raman analysis confirmed that crystallinity remained unchanged during enzymatic cellulose hydrolysis.

Graphical Abstract

Keywords

Biocatalysis Biomaterials Biopolymers Cello-oligomers Endoglucanases Ionic liquid 

Abbreviations

AgrBG

Beta-glucosidases from Agrobacterium sp

AnBG

Beta-glucosidases from A. niger

AnEG

Endoglucanase from A. niger

BaEG

Endoglucanase from B. amyloliquefaciens

BG

Beta-glucosidases

CBH

Cellobiohydrolases

CrI

Crystallinity index

DPW

Weight average degree of polymerization

EG

Endoglucanase

[EMIM] [Ac]

1-Ethyl-3-methylimidazolium acetate

GPC

Gel permeation chromatography

IL

Ionic liquid

MALLS

Multiangle laser light scattering

RI

Refractive index

TeEG

Endoglucanase from T. emersonii

TmEG

Endoglucanase from T. maritima

TlEG

Endoglucanase from T. longibrachiatum

XRD

X-ray diffraction

Notes

Acknowledgments

Parts of this work were performed as part of the Cluster of Excellence “Tailor-Made Fuels from Biomass”, which is funded by the Excellence Initiative by the German federal and state governments to promote science and research at German universities.

Compliance with ethical standards

Conflict of interest

The authors declare no financial or commercial conflict of interest.

Supplementary material

10570_2019_2390_MOESM1_ESM.docx (149 kb)
Supplementary material 1 (DOCX 148 kb)

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Copyright information

© Springer Nature B.V. 2019

Authors and Affiliations

  • Stefanie Kluge
    • 1
    • 2
  • Benjamin Bonhage
    • 1
  • Jörn Viell
    • 3
  • Mari Granström
    • 4
  • Alois Kindler
    • 4
  • Antje C. Spiess
    • 1
    • 2
    • 5
    Email author
  1. 1.AVT - Enzyme Process TechnologyRWTH Aachen UniversityAachenGermany
  2. 2.DWI – Leibniz Institute for Interactive MaterialsAachenGermany
  3. 3.AVT - Process Systems Engineering, RWTH Aachen UniversityAachenGermany
  4. 4.BASF SELudwigshafenGermany
  5. 5.Institute for Biochemical Engineering, TU BraunschweigBraunschweigGermany

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