Design and construction of synthetic cellulosome with three adaptor scaffoldins for cellulosic ethanol production from steam-exploded corn stover
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In this study, a cellulosome-producing yeast reaction system with cellulase and hemicellulase activities is developed for synergistic catalysis of steam-exploded corn stover. Three adaptor scaffoldins with divergent specificities serve to contain an extensive type and amount of catalytic subunits. The maximum ethanol concentration of engineered yeast strain was 0.92 g/l corresponding to 53.44% of the theoretical yield based on grams of ethanol produced per gram of consumed total sugar under 30 °C with agitation at 90 rpm after 96 h. Here, our elaborated structural organization presents an approach designed toward genetic engineering of S. cerevisiae, a widespread, industrially important microorganism, for improved lignocellulolytic potential and advanced capability of consolidated bioprocessing.
KeywordsCellulosome Cellulosic ethanol Steam exploded Corn stover Engineered yeast
This work was supported by the National Natural Science Foundation of China under Grant (No. 31570790); National Key Technology R&D Program under Grant (No. 2013BAD22B03); Capacity Building for Sci-Tech Innovation-Fundamental Scientific Research Funds (No. 025185305000/198).
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