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Cellulose

, Volume 26, Issue 6, pp 3801–3814 | Cite as

Near-complete removal of non-cellulosic components from bamboo by 1-pentanol induced organosolv pretreatment under mild conditions for robust cellulose enzymatic hydrolysis

  • Jing Liu
  • Huichao HuEmail author
  • Zhenggang Gong
  • Guangxu Yang
  • Runqing Li
  • Lihui Chen
  • Liulian Huang
  • Xiaolin LuoEmail author
Original Research
  • 81 Downloads

Abstract

Based on Hildebrand solubility parameter (δH) theory, a novel organosolv pretreatment medium (OPM), 1-pentanol (80%, v/v)–water mixture with a δH value of 27.4 (J/cm3)−1/2, was discovered. This OPM achieved near-complete removal of non-cellulosic components (hemicellulose 96.2% and lignin 87.8%) from bamboo under mild conditions [130 °C, 20 min, 4% (w/w) H2SO4]. For the OPMs with similar δH values [DMSO (80%, v/v)- and 1-pentanol (80%, v/v)-water, 30.9 vs. 27.4 (J/cm3)−1/2] to that of bamboo lignin [28.3 (J/cm3)−1/2] used in this study, the effect of the δh value (contribution of hydrogen-binding force to the δH) on lignin removal was also discussed. After overcoming the negative effect of lignin deposits on enzymes with biocompatible soy protein (SP), a decent cellulose enzymatic conversion (EC) of 92.6% was achieved by 1-pentanol (80%, v/v)-water induced organosolv pretreatment at a low cellulase loading (5 FPU/g glucan). This cellulose EC was much higher than those of other OPMs (< 63%) with the δH values very different from that of bamboo lignin. Integration of organosolv pretreatment with an ideal OPM and enzymatic hydrolysis using SP as an additive can be thereby viewed as a viable way to achieve the robust cellulose EC at low enzyme loading, which shows great potential for improving the economics of organosolv pretreatment-based lignocellulose biorefinery.

Graphical abstract

Keywords

Hildebrand solubility parameter Organosolv pretreatment Non-cellulosic components Enzymatic hydrolysis Soy protein 

Notes

Acknowledgments

This work was supported by National Natural Science Foundation of China (Nos. 31870559 and 31300495), Fujian Provincial Department of Science and Technology (2018J01590; 2019J01387) and Scientific and Technological Innovation Funding of Fujian Agriculture and Forestry University (CXZX2017034, CXZX2017293, CXZX2017374, and CXZX2018004). We also thank Prof. Nairong Chen (FAFU, China) for providing the DSP.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Supplementary material

10570_2019_2334_MOESM1_ESM.docx (340 kb)
Supplementary material 1 (DOCX 340 kb)

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Copyright information

© Springer Nature B.V. 2019

Authors and Affiliations

  1. 1.College of Materials EngineeringFujian Agriculture and Forestry UniversityFuzhouChina

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