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Fabrication of electrospun chitosan/cellulose nanofibers having adsorption property with enhanced mechanical property

  • Duy-Nam Phan
  • Hoik Lee
  • Bijun Huang
  • Yasuhito MukaiEmail author
  • Ick-Soo KimEmail author
Original Research


Chitosan/cellulose (CS/CL) nanofibers were fabricated through electrospinning with a mixture of chitosan (CS) and cellulose acetate (CA) in a co-solvent system (trifluoroacetic/acetic acid) and afterward Na2CO3 treatment was followed. The treatment induces the neutralization of CS and deacetylation of CA, converted into cellulose (CL). The CS/CA nanofiber webs maintained the fibrous structure after treatment (converted into CS/CL nanofibers), which cannot be achieved by CS nanofibers. In addition, the combination of CS and CA enhanced the mechanical properties of the resultant nanofibers up to approximately 17 MPa in tensile strength and 5.5% in elongation at break. More importantly, the resulting nanofibers showed adsorptive characteristics; whereas, CL nanofibers showed no adsorption behavior. The incorporation of CS with CL offers the metal ion adsorption property to the composite nanofibers and gives them a waterproof property, which could be utilized in wastewater purification. The adsorption capacity of CS/CL nanofibers for As(V), Pb(II) and Cu(II) ions reached up to 39.4, 57.3 and 112.6 mg/g. Therefore, this nanofiber system showed effective removal behavior in aqueous solution with reasonable mechanical strength, unattainable with pure CS or CL nanofibers.

Graphical abstract


Cellulose nanofiber Chitosan nanofiber Metal ion adsorption Mechanical property Electrospinning 


Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Supplementary material

10570_2018_2169_MOESM1_ESM.docx (2.7 mb)
Supplementary material 1 (DOCX 2763 kb)


  1. Bhatnagar A, Sillanpää M (2017) Removal of natural organic matter (NOM) and its constituents from water by adsorption—a review. Chemosphere 166:497–510. CrossRefPubMedGoogle Scholar
  2. Bhattarai N, Edmondson D, Veiseh O, Matsen FA, Zhang M (2005) Electrospun chitosan-based nanofibers and their cellular compatibility. Biomaterials 26:6176–6184. CrossRefPubMedGoogle Scholar
  3. Bondarenko O, Juganson K, Ivask A, Kasemets K, Mortimer M, Kahru A (2013) Toxicity of Ag, CuO and ZnO nanoparticles to selected environmentally relevant test organisms and mammalian cells in vitro: a critical review. Arch Toxicol 87:1181–1200. CrossRefPubMedPubMedCentralGoogle Scholar
  4. Buchanan CM, Edgar KJ, Wilson AK (1991) Preparation and characterization of cellulose monoacetates: the relationship between structure and water solubility. Macromolecules 24:3060–3064. CrossRefGoogle Scholar
  5. Coleman DC (1969) “Rayon”, in Courtaulds, vol II. Oxford University Press, London, pp 61–75Google Scholar
  6. Crini G (2005) Recent developments in polysaccharide-based materials used as adsorbents in wastewater treatment. Prog Polym Sci 30:38–70. CrossRefGoogle Scholar
  7. Dąbrowski A, Hubicki Z, Podkościelny P, Robens E (2004) Selective removal of the heavy metal ions from waters and industrial wastewaters by ion-exchange method. Chemosphere 56:91–106. CrossRefPubMedGoogle Scholar
  8. Demirbas E, Kobya M, Senturk E, Ozkan T (2004) Adsorption kinetics for the removal of chromium (VI) from aqueous solutions on the activated carbons prepared from agricultural wastes. Water SA 30:533–539. CrossRefGoogle Scholar
  9. Elsabee MZ, Morsi RE, Al-Sabagh AM (2009) Surface active properties of chitosan and its derivatives. Coll Surf B Biointerfaces 74:1–16. CrossRefGoogle Scholar
  10. Fu F, Wang Q (2011) Removal of heavy metal ions from wastewaters: a review. J Environ Manag 92:407–418. CrossRefGoogle Scholar
  11. Goetz LA, Jalvo B, Rosal R, Mathew AP (2016) Superhydrophilic anti-fouling electrospun cellulose acetate membranes coated with chitin nanocrystals for water filtration. J Membr Sci 510:238–248. CrossRefGoogle Scholar
  12. Griffitt Robert J, Luo J, Gao J, Bonzongo J-C, Barber David S (2009) Effects of particle composition and species on toxicity of metallic nanomaterials in aquatic organisms. Environ Toxicol Chem 27:1972–1978. CrossRefGoogle Scholar
  13. Gupta A, Chauhan VS, Sankararamakrishnan N (2009) Preparation and evaluation of iron–chitosan composites for removal of As(III) and As(V) from arsenic contaminated real life groundwater. Water Res 43:3862–3870. CrossRefPubMedGoogle Scholar
  14. Haider S, Park S-Y (2009) Preparation of the electrospun chitosan nanofibers and their applications to the adsorption of Cu(II) and Pb(II) ions from an aqueous solution. J Membr Sci 328:90–96. CrossRefGoogle Scholar
  15. Heidari A, Younesi H, Mehraban Z, Heikkinen H (2013) Selective adsorption of Pb(II), Cd(II), and Ni(II) ions from aqueous solution using chitosan—MAA nanoparticles. Int J Biol Macromol 61:251–263. CrossRefPubMedGoogle Scholar
  16. Ho Y-S (2006) Second-order kinetic model for the sorption of cadmium onto tree fern: a comparison of linear and non-linear methods. Water Res 40:119–125. CrossRefPubMedGoogle Scholar
  17. Ho Y-S (2014) Using of “pseudo-second-order model” in adsorption. Environ Sci Pollut Res 21:7234–7235. CrossRefGoogle Scholar
  18. Ho YS, McKay G (1998) A comparison of chemisorption kinetic models applied to pollutant removal on various sorbents. Process Saf Environ 76:332–340. CrossRefGoogle Scholar
  19. Hu Y, Liu X, Bai J, Shih K, Zeng EY, Cheng H (2013) Assessing heavy metal pollution in the surface soils of a region that had undergone three decades of intense industrialization and urbanization. Environ Sci Pollut Res 20:6150–6159. CrossRefGoogle Scholar
  20. Hu C, Zhu P, Cai M, Hu H, Fu Q (2017) Comparative adsorption of Pb(II), Cu(II) and Cd(II) on chitosan saturated montmorillonite: kinetic, thermodynamic and equilibrium studies. Appl Clay Sci 143:320–326. CrossRefGoogle Scholar
  21. Huang G, Yang C, Zhang K, Shi J (2009) Adsorptive removal of copper ions from aqueous solution using cross-linked magnetic chitosan beads. Chin J Chem Eng 17:960–966. CrossRefGoogle Scholar
  22. Jayakumar R, Prabaharan M, Nair SV, Tamura H (2010) Novel chitin and chitosan nanofibers in biomedical applications. Biotechnol Adv 28:142–150. CrossRefPubMedGoogle Scholar
  23. Karthik R, Meenakshi S (2015) Removal of Pb(II) and Cd(II) ions from aqueous solution using polyaniline grafted chitosan. Chem Eng J 263:168–177. CrossRefGoogle Scholar
  24. Khatri Z, Wei K, Kim B-S, Kim I-S (2012) Effect of deacetylation on wicking behavior of co-electrospun cellulose acetate/polyvinyl alcohol nanofibers blend. Carbohyd Polym 87:2183–2188. CrossRefGoogle Scholar
  25. Khatri Z, Mayakrishnan G, Hirata Y, Wei K, Kim I-S (2013) Cationic-cellulose nanofibers: preparation and dyeability with anionic reactive dyes for apparel application. Carbohyd Polym 91:434–443. CrossRefGoogle Scholar
  26. Khatri Z, Ahmed F, Jhatial AK, Abro MI, Mayakrishnan G, Kim I-S (2014) Cold pad-batch dyeing of cellulose nanofibers with reactive dyes. Cellulose 21:3089–3095. CrossRefGoogle Scholar
  27. Khatri Z, Ahmed F, Khatri A, Khatri M, Qureshi UA, Kim I-S (2017) Screen-printed electrospun cellulose nanofibers using reactive dyes. Cellulose 24:4561–4568. CrossRefGoogle Scholar
  28. Konwarh R, Karak N, Misra M (2013) Electrospun cellulose acetate nanofibers: the present status and gamut of biotechnological applications. Biotechnol Adv 31:421–437. CrossRefPubMedGoogle Scholar
  29. Krajewska B (2004) Application of chitin- and chitosan-based materials for enzyme immobilizations: a review. Enzyme Microb Technol 35:126–139. CrossRefGoogle Scholar
  30. Krassing HA (1993) Cellulose: structure, accessibility and reactivity. Gordon and Breach Science Publishers, London, pp 41–130Google Scholar
  31. Kumar ASK, Jiang S-J (2016) Chitosan-functionalized graphene oxide: a novel adsorbent an efficient adsorption of arsenic from aqueous solution. J Environ Chem Eng 4:1698–1713. CrossRefGoogle Scholar
  32. Lee JW et al (2012) Korea national survey for environmental pollutants in the human body 2008: heavy metals in the blood or urine of the Korean population. Int J Hyg Environ Health 215:449–457. CrossRefPubMedGoogle Scholar
  33. Lee DW, Lim C, Israelachvili JN, Hwang DS (2013) Strong adhesion and cohesion of chitosan in aqueous solutions. Langmuir 29:14222–14229. CrossRefPubMedPubMedCentralGoogle Scholar
  34. Lee H, Koo JM, Sohn D, Kim I-S, Im SS (2016a) High thermal stability and high tensile strength terpolyester nanofibers containing biobased monomer: fabrication and characterization. RSC Adv 6:40383–40388. CrossRefGoogle Scholar
  35. Lee H, Watanabe K, Kim M, Gopiraman M, Song K-H, Lee JS, Kim IS (2016b) Handspinning enabled highly concentrated carbon nanotubes with controlled orientation in nanofibers. Sci Rep 6:37590. CrossRefPubMedPubMedCentralGoogle Scholar
  36. Lee H, Nishino M, Sohn D, Lee JS, Kim IS (2018) Control of the morphology of cellulose acetate nanofibers via electrospinning. Cellulose 25:2829–2837. CrossRefGoogle Scholar
  37. Li N, Bai R (2005) Copper adsorption on chitosan–cellulose hydrogel beads: behaviors and mechanisms. Sep Purif Technol 42:237–247. CrossRefGoogle Scholar
  38. Li H, Zhu C, Xue J, Ke Q, Xia Y (2017) Enhancing the mechanical properties of electrospun nanofiber mats through controllable welding at the cross points. Macromol Rapid Commun 38:1600723. CrossRefGoogle Scholar
  39. Lim C, Lee DW, Israelachvili JN, Jho Y, Hwang DS (2015) Contact time- and pH-dependent adhesion and cohesion of low molecular weight chitosan coated surfaces. Carbohyd Polym 117:887–894. CrossRefGoogle Scholar
  40. Liu C, Bai R (2005) Preparation of chitosan/cellulose acetate blend hollow fibers for adsorptive performance. J Membr Sci 267:68–77. CrossRefGoogle Scholar
  41. Liu XD, Nishi N, Tokura S, Sakairi N (2001) Chitosan coated cotton fiber: preparation and physical properties. Carbohydr Polym 44:233–238. CrossRefGoogle Scholar
  42. Luo X, Zeng J, Liu S, Zhang L (2015) An effective and recyclable adsorbent for the removal of heavy metal ions from aqueous system: magnetic chitosan/cellulose microspheres. Bioresour Technol 194:403–406. CrossRefPubMedGoogle Scholar
  43. Mahmoodi NM, Mokhtari-Shourijeh Z (2015) Preparation of PVA-chitosan blend nanofiber and its dye removal ability from colored wastewater. Fiber Polym 16:1861–1869. CrossRefGoogle Scholar
  44. McAfee BJ, Gould WD, Nadeau JC, da Costa ACA (2001) Biosorption of metal ions using chitosan, chitin, and biomass of Rhizopus Oryzae. Sep Sci Technol 36:3207–3222. CrossRefGoogle Scholar
  45. Mi F-L, Shyu S-S, Kuan C-Y, Lee S-T, Lu K-T, Jang S-F (1999) Chitosan-Polyelectrolyte complexation for the preparation of gel beads and controlled release of anticancer drug. I. Effect of phosphorous polyelectrolyte complex and enzymatic hydrolysis of polymer. J Appl Polym Sci 74:1868–1879.;2-N CrossRefGoogle Scholar
  46. Min B-M, Lee SW, Lim JN, You Y, Lee TS, Kang PH, Park WH (2004) Chitin and chitosan nanofibers: electrospinning of chitin and deacetylation of chitin nanofibers. Polymer 45:7137–7142. CrossRefGoogle Scholar
  47. Mohammed Abdul KS, Jayasinghe SS, Chandana EPS, Jayasumana C, De Silva PMCS (2015) Arsenic and human health effects: a review. Environ Toxicol Pharmacol 40:828–846. CrossRefGoogle Scholar
  48. Mohan D, Pittman CU (2006) Activated carbons and low cost adsorbents for remediation of tri- and hexavalent chromium from water. J Hazard Mater 137:762–811. CrossRefPubMedGoogle Scholar
  49. Nasreen SAAN, Sundarrajan S, Syed Nizar SA, Balamurugan R, Ramakrishna S (2013) In situ polymerization of PVDF-HEMA polymers: electrospun membranes with improved flux and antifouling properties for water filtration. Polym J 46:167–174. CrossRefGoogle Scholar
  50. Neghlani PK, Rafizadeh M, Taromi FA (2011) Preparation of aminated-polyacrylonitrile nanofiber membranes for the adsorption of metal ions: comparison with microfibers. J Hazard Mater 186:182–189. CrossRefPubMedGoogle Scholar
  51. Qu X, Wirsén A, Albertsson AC (2000) Novel pH-sensitive chitosan hydrogels: swelling behavior and states of water. Polymer 41:4589–4598. CrossRefGoogle Scholar
  52. Saha S, Sarkar P (2012) Arsenic remediation from drinking water by synthesized nano-alumina dispersed in chitosan-grafted polyacrylamide. J Hazard Mater 227–228:68–78. CrossRefPubMedGoogle Scholar
  53. Salihu G, Goswami P, Russell S (2012) Hybrid electrospun nonwovens from chitosan/cellulose acetate. Cellulose 19:739–749. CrossRefGoogle Scholar
  54. Srbová J, Slováková M, Křípalová Z, Žárská M, Špačková M, Stránská D, Bílková Z (2016) Covalent biofunctionalization of chitosan nanofibers with trypsin for high enzyme stability. React Funct Polym 104:38–44. CrossRefGoogle Scholar
  55. Suedel BC, Deaver E, Rodgers JH (1996) Experimental factors that may affect toxicity of aqueous and sediment-bound copper to freshwater organisms. Arch Environ Contam Toxicol 30:40–46. CrossRefGoogle Scholar
  56. Vasudevan S, Lakshmi J, Sozhan G (2009) Studies on the removal of iron from drinking water by electrocoagulation—a clean process. Clean Soil Air Water 37:45–51. CrossRefGoogle Scholar
  57. Vu D, Li X, Wang C (2013) Adsorption of As(III) from aqueous solution based on porous magnetic/chitosan/ferric hydroxide microspheres prepared via electrospraying. Sci China Chem 56:678–684. CrossRefGoogle Scholar
  58. Wahab JA, Xu G, Lee H, Nam PD, Wei K, Kim SH, Kim IS (2016) Fabrication of silk fibroin/eggshell nanofiber membranes for facemasks. Fiber Polym 17:1776–1781. CrossRefGoogle Scholar
  59. Wan Ngah WS, Hanafiah MAKM (2008) Removal of heavy metal ions from wastewater by chemically modified plant wastes as adsorbents: a review. Bioresour Technol 99:3935–3948. CrossRefPubMedGoogle Scholar
  60. Wan M-W, Kan C-C, Rogel BD, Dalida MLP (2010) Adsorption of copper (II) and lead (II) ions from aqueous solution on chitosan-coated sand. Carbohyd Polym 80:891–899. CrossRefGoogle Scholar
  61. Wang J, Chen C (2014) Chitosan-based biosorbents: modification and application for biosorption of heavy metals and radionuclides. Bioresour Technol 160:129–141. CrossRefPubMedGoogle Scholar
  62. Wang J, Pan K, He Q, Cao B (2013) Polyacrylonitrile/polypyrrole core/shell nanofiber mat for the removal of hexavalent chromium from aqueous solution. J Hazard Mater 244–245:121–129. CrossRefPubMedGoogle Scholar
  63. Yang B-Y, Cao Y, Qi F-F, Li X-Q, Xu Q (2015) Atrazine adsorption removal with nylon6/polypyrrole core-shell nanofibers mat: possible mechanism and characteristics. Nanoscale Res Lett 10:207. CrossRefPubMedPubMedCentralGoogle Scholar
  64. Yao Y et al (2016) Carbon welding by ultrafast joule heating. Nano Lett 16:7282–7289. CrossRefPubMedGoogle Scholar
  65. Yin C, Jatoi AW, Bang H, Gopiraman M, Kim IS (2016) Fabrication of silk fibroin based three dimensional scaffolds for tissue engineering. Fiber Polym 17:1140–1145. CrossRefGoogle Scholar
  66. Yu R, Yuan X, Zhao Y, Hu G, Tu X (2008) Heavy metal pollution in intertidal sediments from Quanzhou Bay. China J Environ Sci 20:664–669. CrossRefGoogle Scholar
  67. Yu D-G, Yu J-H, Chen L, Williams GR, Wang X (2012) Modified coaxial electrospinning for the preparation of high-quality ketoprofen-loaded cellulose acetate nanofibers. Carbohyd Polym 90:1016–1023. CrossRefGoogle Scholar
  68. Zeng L et al (2015) Adsorption of Cd(II), Cu(II) and Ni(II) ions by cross-linking chitosan/rectorite nano-hybrid composite microspheres. Carbohyd Polym 130:333–343. CrossRefGoogle Scholar
  69. Zhang M, Li XH, Gong YD, Zhao NM, Zhang XF (2002) Properties and biocompatibility of chitosan films modified by blending with PEG. Biomaterials 23:2641–2648. CrossRefPubMedGoogle Scholar
  70. Zhang L, Menkhaus TJ, Fong H (2008) Fabrication and bioseparation studies of adsorptive membranes/felts made from electrospun cellulose acetate nanofibers. J Membr Sci 319:176–184. CrossRefGoogle Scholar
  71. Zhu Y, Hu J, Wang J (2012) Competitive adsorption of Pb(II), Cu(II) and Zn(II) onto xanthate-modified magnetic chitosan. J Hazard Mater 221–222:155–161. CrossRefPubMedGoogle Scholar
  72. Zhu C, Liu F, Zhang Y, Wei M, Zhang X, Ling C, Li A (2016) Nitrogen-doped chitosan-Fe(III) composite as a dual-functional material for synergistically enhanced co-removal of Cu(II) and Cr(VI) based on adsorption and redox. Chem Eng J 306:579–587. CrossRefGoogle Scholar

Copyright information

© Springer Nature B.V. 2018

Authors and Affiliations

  1. 1.Nano Fusion Technology Research Group, Division of Frontier Fibers, Institute for Fiber Engineering (IFES), Interdisciplinary Cluster for Cutting Edge Research (ICCER)Shinshu UniversityUedaJapan
  2. 2.Department of Chemical Systems EngineeringNagoya UniversityNagoyaJapan

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