Rapid quantification of cellulose nanocrystals by Calcofluor White fluorescence staining
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Cellulose nanocrystals (CNCs) have gained increased interest worldwide for their unique properties. CNC production by acid hydrolysis of cellulose-rich biomass is well established, but its' quantification is still complicated. In this study, a rapid method for the determination of CNC concentration using Calcofluor White (CW) fluorescence dye is demonstrated for both purified and homemade CNCs recovered from Whatman filter paper. The method is robust, selective for crystalline cellulose, suitable for routine measurement in CNC production and that the pH must be basic for staining using CW.
KeywordsCellulose nanocrystals (CNC) quantification Calcofluor White Fluorescence Whatman filter paper quantification
This research was conducted in the framework of the Israeli Ministry of National Infrastructure, Energy and Water Resources Grant No. 214-11-006 and the Israeli Ministry of Environmental Protection Grant No. 132-3-4.
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