Effect of bamboo fiber on the degradation behavior and in vitro cytocompatibility of the nano-hydroxyapatite/poly(lactide-co-glycolide) (n-HA/PLGA) composite
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To obtain an ideal nano-hydroxyapatite/poly(lactide-co-glycolide) (n-HA/PLGA) composite for bone materials, bamboo fiber (BF) was selected to reinforce n-HA/PLGA. The effects of different loadings and surface modification methods for BF on in vitro degradation behavior and cell viability of n-HA/PLGA were evaluated. In vitro degradation was tested in a simulated body fluid while cell viability experiment was carried out using human osteoblast-like cells (MG-63). The changes of tensile strength, water absorption, surface microstructure and thermal properties during the soaking were investigated by means of electromechanical universal tester, scanning electron microscope and differential scanning calorimeter. Moreover, the cell attachment and proliferation of samples were evaluated by fluorescence microscope, inverted microscope and MTT method. The results showed that the degradation started from the interface between BF and n-HA/PLGA matrix, and the degradation rate of the BF/n-HA/PLGA composite was accelerated with the increasing BF amount. Fortunately, the surface-modified BF could improve the interface compatibility and display more suitable degradation rate. In addition, the in vitro cell incubating experimental results reflected that BF was nontoxic and had no side effect on cell attachment and proliferation. Especially, BF/n-HA/PLGA composite with 5% surface-modified BF had better cytocompatibility. The study suggested that the surface-modified BF had a great potential in developing BF/n-HA/PLGA composite used as bone materials in the future.
KeywordsBamboo fiber Hydroxyapatite Polymer Biocompatibility Biodegradation
The authors would like to acknowledge the support of Natural Science Foundation of Hunan Province (Grant No. 2017JJ2179), the Innovation Platform Open Found of Hunan Provincial Education Department (Grant No.17K055), and Opening Found of Key Laboratory of C Key Laboratory of Chemical Biology and Traditional Chinese Medicine Research (Ministry of Education of China), Hunan Normal University (No. KLCBTCMR201812).
- Jiang LX, Jiang LY, Ma Han CT, Xu LJ, Xiong CD (2013) Preparation and characterization of nano-hydroxyapatite/PLGA composites with novel surface-modified nano-hydroxyapatite. J Inorg Mater 28:751–756Google Scholar