Three-dimensional bacterial cellulose-electrospun membrane hybrid structures fabricated through in-situ self-assembly
- 303 Downloads
In this work, bacterial cellulose/electrospun membrane three-dimensional (BC/ENM 3D) hybrid structures were fabricated using in-situ self-assembly and characterized by SEM and FTIR. SEM showed as the bacterium extrudes cellulose nanofibrils; they gradually self-assemble to form a unified network on membrane surface and also penetrate into its structure, hence securely binding electrospun nanofiber and interlocking multiple membrane layers to form a stable 3D hybrid scaffold. FTIR results confirmed the presence of BC in the resulting nanocomposites. The permeability and porosity of the scaffold decreased with prolonged fermentation and with increased oxygen ratio as the cellulose grew more quickly.
KeywordsBacterial cellulose In-situ self-assembly 3D hybrid structures Nanofibers Scaffolds
This work was supported by the national first-class discipline program of Light Industry Technology and Engineering (LITE2018-21), and the 111 Project (B17021).
Compliance with ethical standards
Conflicts of interest
The authors declare no conflict of interest.
- Bäckdahl H, Helenius G, Bodin A, Nannmark U, Johansson BR, Risberg B, Gatenholm P (2006) Mechanical properties of bacterial cellulose and interactions with smooth muscle cells. Biomaterials 27(9):2141–2149. https://doi.org/10.1016/j.biomaterials.2005.10.026 CrossRefPubMedGoogle Scholar
- Bielecki SKA, Turkiewicz M, Kalinowska H (2005) Bacterial cellulose. Polysaccharides I: polysaccharides from prokaryotes. In: Vandamme E, De Baets S (eds) Biopolymers online. Wiley-VCH, Weinheim, pp 37–46Google Scholar
- Bodin A, Bharadwaj S, Wu S, Gatenholm P, Atala A, Zhang Y (2010) Tissue-engineered conduit using urine-derived stem cells seeded bacterial cellulose polymer in urinary reconstruction and diversion. Biomaterials 31(34):901–8889. https://doi.org/10.1016/j.biomaterials.2010.07.108 CrossRefGoogle Scholar
- Czaja W, Krystynowicz A, Bielecki S, Brown RM Jr (2006) Microbial cellulose—the natural power to heal wounds. Biomaterials 27(2):51–145. https://doi.org/10.1016/j.biomaterials.2005.07.035 CrossRefGoogle Scholar
- Gatenholm, Bodin A, Bäckdahl H, Gustafsson L, Bo Risberg P (2006) Manufacturing and characterization of bacterial cellulose tubes using two different fermentation Techniques. In: Mendez-Vilas A (ed) Modern multidisciplinary applied microbiology. Wiley-VCH, Weinheim, pp 22–619Google Scholar
- Hirayama K, Okitsu T, Teramae H, Kiriya D, Onoe H, Takeuchi S (2013) Cellular building unit integrated with microstrand-shaped bacterial cellulose. Biomaterials 34(10):2421–2427. https://doi.org/10.1016/j.biomaterials.2012.12.013 CrossRefPubMedGoogle Scholar
- Hoenich N (2006) Cellulose for medical applications: past, present, and future. BioResources 1(2):270–280Google Scholar
- Levesque SG, Lim RM, Shoichet MS (2005) Macroporous interconnected dextran scaffolds of controlled porosity for tissue-engineering applications. Biomaterials 26(35):46–7436. https://doi.org/10.1016/j.biomaterials.2005.05.054 CrossRefGoogle Scholar
- Oliveira Barud HG, Barud Hda S, Cavicchioli M, do Amaral TS, de Oliveira Junior OB, Santos DM, Petersen AL, Celes F, Borges VM, de Oliveira CI, de Oliveira PF, Furtado RA, Tavares DC, Ribeiro SJ (2015) Preparation and characterization of a bacterial cellulose/silk fibroin sponge scaffold for tissue regeneration. Carbohydr Polym 128:41–51. https://doi.org/10.1016/j.carbpol.2015.04.007 CrossRefPubMedGoogle Scholar
- Schramm M, Hestrin S (1954) Factors affecting production of cellulose at the air/liquid interface of a culture of acetobacter xylinum. Microbiology 11:123–129Google Scholar
- Wippermann J, Schumann D, Klemm D, Kosmehl H, Salehi-Gelani S, Wahlers T (2009) Preliminary results of small arterial substitute performed with a new cylindrical biomaterial composed of bacterial cellulose. Eur J Vasc Endovasc Surg 37(5):592–596. https://doi.org/10.1016/j.ejvs.2009.01.007 CrossRefPubMedGoogle Scholar
- Zahedmanesh H, Mackle JN, Sellborn A, Drotz K, Bodin A, Gatenholm P, Lally C (2011) Bacterial cellulose as a potential vascular graft: mechanical characterization and constitutive model development. J Biomed Mater Res B Appl Biomater 97(1):13–105. https://doi.org/10.1002/jbm.b.31791 CrossRefGoogle Scholar