Parameters of hydrothermal gelation of chitin nanofibers determined using a severity factor
- 135 Downloads
The hydrothermal gelation of α- and β-chitin nanofibers (α- and β-ChNFs) prepared at neutral and acidic pH was conducted by heating them to 120, 160, 180, and 200 °C in a sealed reactor. The optical transmittance and mechanical strength of β-ChNFs gelated at the acidic pH were determined for the first time using a severity factor defined as a function of the integrated heating time and temperature. The width of β-ChNFs increased after the hydrothermal treatment, indicating that these fibers strongly adhered to each other to form a network structure during gelation. Furthermore, the hydrothermal gelation of α- and β-ChNFs with different degrees of disintegration prepared at the neutral and acidic pH was conducted. It was found that the hydrothermal treatment of α-chitin must be performed at the acidic pH to obtain a self-sustaining hydrogel of well-disintegrated NFs. The disintegration of β-chitin into NFs occurred more easily at the acidic pH than under the neutral conditions; however, in the latter case, the same disintegration degree of β-ChNFs could be achieved by increasing the number of disintegration steps. At the same disintegration degree, the strength of the self-sustaining hydrogel obtained at the neutral conditions was greater than that of the gel prepared at the acidic pH, indicating that the electrostatic repulsion caused by acid addition negatively affected the formation of the hydrogel network structure. To maximize the efficiency of the hydrothermal gelation process, ChNFs should be as thin as possible and electrostatic repulsion forces must be controlled.
Keywordsα-Chitin nanofiber β-Chitin nanofiber Hydrothermal gelation Severity factor
This work was supported by JSPS KAKENHI [Grant No.: 17H04893]. We thank Dr. Nobuhide Takahashi, Dr. Hiroshi Fukunaga, Dr. Iori Shimada, Dr. Kazuhide Totani, Dr. Yoshihiro Nomura, and Mr. Kazuhiko Yamashita for their substantial intellectual contributions to the conception of the project.
Compliance with ethical standards
Conflict of interest
The authors declare that they have no conflict of interest.
- Izumi R, Komada S, Ochi K, Karasawa L, Osaki T, Murahata Y, Tsuka T, Imagawa T, Itoh N, Okamoto Y, Izawa H, Morimoto M, Saimoto H, Azuma K, Ifuku S (2015) Favorable effects of superficially deacetylated chitin nanofibrils on the wound healing process. Carbohydr Polym 123:461–467CrossRefGoogle Scholar
- Sowmya S, Kumar PTS, Chennazhi KP, Nair SV, Tamura H, Jayakumar R (2011) Biocompatible β-chitin hydrogel/nanobioactive glass ceramic nanocomposite scaffolds for periodontal bone regeneration. Trends Biomater Artif Organs 25:1–11Google Scholar
- Suenaga S, Osada M (2018a) Self-sustaining cellulose nanofiber hydrogel produced by hydrothermal gelation without additives. ACS Biomater Sci Eng 4:1536–1545Google Scholar