, Volume 25, Issue 8, pp 4677–4694 | Cite as

Effects of pretreatment and enzyme cocktail composition on the sugars production from oil palm empty fruit bunch fiber (OPEFBF)

  • Pei-Ling TangEmail author
  • Peer Mohamed Abdul
  • Nurul Sakinah Engliman
  • Osman Hassan
Original Paper


This research was conducted to investigate the interaction effects of pretreatment (steam, acetic acid–steam, acetic acid–glycerol) and enzyme cocktail composition [cellulases C:V:N (Celluclast® 1.5 L: Viscozyme® L: Novozyme® 188), cellulase–hemicellulase CC:CH (Cellic® Ctec 2: Cellic® Htec 2)] on the sugars (xylose, glucose) production from oil palm empty fruit bunch fiber (OPEFBF). The mix ratio of enzyme cocktails which led to optimum enzymatic saccharification of different pretreated OPEFBs were determined through Simplex Lattice mixture design. By using a cocktail of cellulolytic enzyme C:N at the ratio of 7.8:2.2, about 563.34 mg sugars g−1 carbohydrate was recovered from the acetic acid–glycerol pretreated OPEFBF. However, about 696.92 mg sugars g−1 carbohydrate was successfully produced from acetic acid–steam pretreated OPEFBF by using a cocktail of cellulase–hemicellulase enzyme (CC:CH = 6.3:3.7). Upon optimization using RSM, about 96% of xylose (960.47 ± 102.53 mg xylose g−1 xylan) and 85% of glucose (845.75 ± 55.91 mg glucose g−1 glucan) have been recovered from 3% (w/v) glucan of acetic acid–steam pretreated OPEFBF by adding 1.39% (v/v) CC:CH enzyme cocktail (35.19 mg protein g−1 glucan loading) in a 48-h reaction. CC:CH enzyme cocktail has been proved to be the best enzyme cocktail to use in OPEFBF saccharification for high sugars hydrolysate production.

Graphical abstract


Oil palm empty fruit bunch fiber (OPEFBF) Enzymatic saccharification Cellulase Hemicellulase Pretreatment 



We are grateful for the financial support of this research from Ministry of Science, Technology and Innovation (MOSTI) under grant 10-05-MGI-GMB001.


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Copyright information

© Springer Nature B.V. 2018

Authors and Affiliations

  1. 1.Department of Bioscience, Faculty of Applied SciencesTunku Abdul Rahman University CollegeSetapakMalaysia
  2. 2.Department of Chemical and Process Engineering, Faculty of Engineering and Built EnvironmentUniversiti Kebangsaan MalaysiaBangiMalaysia
  3. 3.Department of Chemical Sciences and Food Technology, Faculty of Science and TechnologyUniversiti Kebangsaan MalaysiaBangiMalaysia

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