, Volume 25, Issue 3, pp 1571–1581 | Cite as

Physical structure variations of bacterial cellulose produced by different Komagataeibacter xylinus strains and carbon sources in static and agitated conditions

  • Pratyawadee Singhsa
  • Ravin Narain
  • Hathaikarn Manuspiya
Original Paper


The morphology, crystallinity, crystallite size, and production yield of bacterial cellulose (BC) produced with six different carbon sources (glucose, fructose, lactose, maltitol, sucralose, and xylitol) in static and agitated fermentation conditions by five strains of Komagataeibacter xylinus (KX, TISTR 086, 428, 975, and 1011) which are locally available, were studied. In static condition, the BC pellicle was formed as a membrane sheet at the medium surface exposed to air, while in agitated condition, the spherical or asterisk-like shape BC particles were obtained in the culture media. The XRD and FT-IR analyses found no significant differences in the cellulose crystallinity, crystallite size or polymorphic distribution within the carbon sources. However, changes in crystallinity and mass fraction of the Iα allomorph were observed in BC produced from the different bacterial strains and incubation conditions. The BC samples produced by the same bacterial strain with the varying culture conditions showed the alteration of physical properties more clearly than the BC samples prepared by the opposite situation. These findings suggested that the strains of bacteria and fermentation conditions strongly affected on the physical structures of BC.


Bacterial cellulose Komagataeibacter xylinus Agitated culture Carbon source Morphology Crystallinity 



The Petroleum and Petrochemical College, Chulalongkorn University was thanked for supporting. This research was funded by the Doctoral Degree Chulalongkorn University 100th Year Birthday Anniversary Scholarship, and the Ratchadapisek Sompoch Endowment Fund (2016), Chulalongkorn University (CU-59-026-AM).


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© Springer Science+Business Media B.V., part of Springer Nature 2018

Authors and Affiliations

  1. 1.The Petroleum and Petrochemical College, Center of Excellence on Petrochemical and Materials TechnologyChulalongkorn UniversityBangkokThailand
  2. 2.Department of Chemical and Materials EngineeringDonadeo Innovation Centre for EngineeringEdmontonCanada

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