Physical structure variations of bacterial cellulose produced by different Komagataeibacter xylinus strains and carbon sources in static and agitated conditions Original Paper First Online: 07 February 2018 Received: 02 December 2017 Accepted: 03 February 2018 Abstract
The morphology, crystallinity, crystallite size, and production yield of bacterial cellulose (BC) produced with six different carbon sources (glucose, fructose, lactose, maltitol, sucralose, and xylitol) in static and agitated fermentation conditions by five strains of
Komagataeibacter xylinus (KX, TISTR 086, 428, 975, and 1011) which are locally available, were studied. In static condition, the BC pellicle was formed as a membrane sheet at the medium surface exposed to air, while in agitated condition, the spherical or asterisk-like shape BC particles were obtained in the culture media. The XRD and FT-IR analyses found no significant differences in the cellulose crystallinity, crystallite size or polymorphic distribution within the carbon sources. However, changes in crystallinity and mass fraction of the I α allomorph were observed in BC produced from the different bacterial strains and incubation conditions. The BC samples produced by the same bacterial strain with the varying culture conditions showed the alteration of physical properties more clearly than the BC samples prepared by the opposite situation. These findings suggested that the strains of bacteria and fermentation conditions strongly affected on the physical structures of BC. Keywords Bacterial cellulose Komagataeibacter xylinus Agitated culture Carbon source Morphology Crystallinity Notes Acknowledgments
The Petroleum and Petrochemical College, Chulalongkorn University was thanked for supporting. This research was funded by the Doctoral Degree Chulalongkorn University 100th Year Birthday Anniversary Scholarship, and the Ratchadapisek Sompoch Endowment Fund (2016), Chulalongkorn University (CU-59-026-AM).
Atalla RH, Vanderhart DL (1984) Native cellulose: a composite of two cistinct crystalline forms. Science 223:283–285.
https://doi.org/10.1126/science.223.4633.283 CrossRef Google Scholar
Benziman M, Haigler CH, Brown RM, White AR, Cooper KM (1980) Cellulose biogenesis: polymerization and crystallization are coupled processes in
. Proc Natl Acad Sci U S A 77:6678–6682
CrossRef Google Scholar
Bi JC, Liu SX, Li CF, Li J, Liu LX, Deng J, Yang YC (2014) Morphology and structure characterization of bacterial celluloses produced by different strains in agitated culture. J Appl Microbiol 117:1305–1311.
https://doi.org/10.1111/jam.12619 CrossRef Google Scholar
Brown RM (2004) Cellulose structure and biosynthesis: what is in store for the 21st century? J Polym Sci Part A Polym Chem 42:487–495.
https://doi.org/10.1002/pola.10877 CrossRef Google Scholar
Budhiono A, Rosidi B, Taher H, Iguchi M (1999) Kinetic aspects of bacterial cellulose formation in nata-de-coco culture system. Carbohydr Polym 40:137–143.
https://doi.org/10.1016/S0144-8617(99)00050-8 CrossRef Google Scholar
Castro C, Zuluaga R, Putaux J-L, Caro G, Mondragon I, Gañán P (2011) Structural characterization of bacterial cellulose produced by
sp. from Colombian agroindustrial wastes. Carbohydr Polym 84:96–102.
https://doi.org/10.1016/j.carbpol.2010.10.072 CrossRef Google Scholar
Chang C, Zhang L (2011) Cellulose-based hydrogels: present status and application prospects. Carbohydr Polym 84:40–53.
https://doi.org/10.1016/j.carbpol.2010.12.023 CrossRef Google Scholar
Cheng K-C, Catchmark JM, Demirci A (2009) Enhanced production of bacterial cellulose by using a biofilm reactor and its material property analysis. J Biol Eng 3:12.
https://doi.org/10.1186/1754-1611-3-12 CrossRef Google Scholar
Colvin JR, Leppard GG (1977) The biosynthesis of cellulose by
. Can J Microbiol 23:701–709.
https://doi.org/10.1139/m77-105 CrossRef Google Scholar
Czaja W, Romanovicz D, Rm Brown (2004) Structural investigations of microbial cellulose produced in stationary and agitated culture. Cellulose 11:403–411.
https://doi.org/10.1023/b:cell.0000046412.11983.61 CrossRef Google Scholar
Delmer DP, Amor Y (1995) Cellulose biosynthesis. Plant Cell 7:987–1000
CrossRef Google Scholar
Dudman WF (1960) Cellulose production by
strains in submerged culture. Microbiology 22:25–39.
https://doi.org/10.1099/00221287-22-1-25 Google Scholar
French AD (2014) Idealized powder diffraction patterns for cellulose polymorphs. Cellulose 21:885–896.
https://doi.org/10.1007/s10570-013-0030-4 CrossRef Google Scholar
Gindl W, Keckes J (2004) Tensile properties of cellulose acetate butyrate composites reinforced with bacterial cellulose. Compos Sci Technol 64:2407–2413.
https://doi.org/10.1016/j.compscitech.2004.05.001 CrossRef Google Scholar
Hestrin S, Schramm M (1954) Synthesis of cellulose by acetobacter xylinum. 2. Preparation of freeze-dried cells capable of polymerizing glucose to cellulose. Biochem J 58:345–352
CrossRef Google Scholar
Hirai A, Tsuji M, Horii F (2002) TEM study of band-like cellulose assemblies produced by acetobacter xylinum at 4 C. Cellulose 9:105–113.
https://doi.org/10.1023/a:1020195205030 CrossRef Google Scholar
Hu Y, Catchmark JM (2011) Integration of cellulases into bacterial cellulose: toward bioabsorbable cellulose composites. J Biomed Mater Res Part B 97B:114–123.
https://doi.org/10.1002/jbm.b.31792 CrossRef Google Scholar
Iguchi M, Yamanaka S, Budhiono A (2000) Bacterial cellulose—a masterpiece of nature’s arts. J Mater Sci 35:261–270.
https://doi.org/10.1023/a:1004775229149 CrossRef Google Scholar
Jahn CE, Selimi DA, Barak JD, Charkowski AO (2011) The Dickeya dadantii biofilm matrix consists of cellulose nanofibres, and is an emergent property dependent upon the type III secretion system and the cellulose synthesis operon. Microbiology 157:2733–2744.
https://doi.org/10.1099/mic.0.051003-0 CrossRef Google Scholar
Keshk S, Razek T, Sameshima K (2006) Bacterial cellulose production from beet molasses. Afr J Biotechnol 5:1519–1523
Klemm D, Heublein B, Fink H-P, Bohn A (2005) Cellulose: fascinating biopolymer and sustainableraw material. Angew Chem Int Ed 44:3358–3393.
https://doi.org/10.1002/anie.200460587 CrossRef Google Scholar
Krystynowicz A, Czaja W, Wiktorowska-Jezierska A, Gonçalves-Miśkiewicz M, Turkiewicz M, Bielecki S (2002) Factors affecting the yield and properties of bacterial cellulose. J Ind Microbiol Biotech 29:189–195.
https://doi.org/10.1038/sj.jim.7000303 CrossRef Google Scholar
Lee H, Zhao X (1999) Effects of mixing conditions on the production of microbial cellulose by
. Biotechnol Bioprocess Eng 4:41–45.
https://doi.org/10.1007/bf02931912 CrossRef Google Scholar
Mihranyan A, Llagostera AP, Karmhag R, Strømme M, Ek R (2004) Moisture sorption by cellulose powders of varying crystallinity. Int J Pharm 269:433–442.
https://doi.org/10.1016/j.ijpharm.2003.09.030 CrossRef Google Scholar
Mohammadkazemi F, Azin M, Ashori A (2015) Production of bacterial cellulose using different carbon sources and culture media. Carbohydr Polym 117:518–523.
https://doi.org/10.1016/j.carbpol.2014.10.008 CrossRef Google Scholar
Mohite B, Salunke B, Patil S (2013) Enhanced production ofbacterial cellulose by using
NCIM 2529 strain under shaking conditions. Appl Biochem Biotechnol 169:1497–1511.
https://doi.org/10.1007/s12010-013-0092-7 CrossRef Google Scholar
Morgan JLW, Strumillo J, Zimmer J (2013) Crystallographic snapshot of cellulose synthesis and membrane translocation. Nature 493:181–186.
https://doi.org/10.1038/nature11744 CrossRef Google Scholar
Nakagaito AN, Iwamoto S, Yano H (2005) Bacterial cellulose: the ultimate nano-scalar cellulose morphology for the production of high-strength composites. Appl Phys A 80:93–97.
https://doi.org/10.1007/s00339-004-2932-3 CrossRef Google Scholar
Nguyen VT, Gidley MJ, Dykes GA (2008) Potential of a nisin-containing bacterial cellulose film to inhibit
on processed meats. Food Microbiol 25:471–478.
https://doi.org/10.1016/j.fm.2008.01.004 CrossRef Google Scholar
Omran A, Ahearn G, Bowers D, Swenson J, Coughlin C (2013) Metabolic effects of sucralose on environmental bacteria. J Toxicol 2013:6.
https://doi.org/10.1155/2013/372986 CrossRef Google Scholar
Ross P, Mayer R, Benziman M (1991) Cellulose biosynthesis and function in bacteria. Microbiol Rev 55:35–58
Ruka DR, Simon GP, Dean KM (2012) Altering the growth conditions of
to maximize the yield of bacterial cellulose. Carbohydr Polym 89:613–622.
https://doi.org/10.1016/j.carbpol.2012.03.059 CrossRef Google Scholar
Schramm M, Gromet Z, Hestrin S (1957) Synthesis of cellulose by
. 3. Substrates and inhibitors. Biochem J 67:669–679
CrossRef Google Scholar
Tokoh C, Takabe K, Fujita M, Saiki H (1998) Cellulose synthesized by
in the presence of acetyl glucomannan. Cellulose 5:249–261.
https://doi.org/10.1023/a:1009211927183 CrossRef Google Scholar
Tsekos I (1999) The sites of cellulose synthesis in algae: diversity and evolution of cellulose-synthesizing enzyme complexes. J Phycol 35:635–655.
https://doi.org/10.1046/j.1529-8817.1999.3540635.x CrossRef Google Scholar
Watanabe K, Tabuchi M, Morinaga Y, Yoshinaga F (1998) Structural features andproperties of bacterial cellulose produced in agitated culture. Cellulose 5:187–200.
https://doi.org/10.1023/a:1009272904582 CrossRef Google Scholar
Yamada Y, Yukphan P, Lan Vu HT, Muramatsu Y, Ochaikul D, Tanasupawat S, Nakagawa Y (2012) Description of
gen. nov., with proposals of new combinations (
). J Gen Appl Microbiol 58:397–404.
https://doi.org/10.2323/jgam.58.397 CrossRef Google Scholar
Yamamoto H, Horii F, Hirai A (1996) In situ crystallization of bacterial cellulose II. Influences of different polymeric additives on the formation of celluloses I
at the early stage of incubation. Cellulose 3:229–242.
https://doi.org/10.1007/bf02228804 CrossRef Google Scholar
Yamanaka S, Watanabe K, Kitamura N, Iguchi M, Mitsuhashi S, Nishi Y, Uryu M (1989) The structure and mechanical properties of sheets prepared from bacterial cellulose. J Mater Sci 24:3141–3145.
https://doi.org/10.1007/bf01139032 CrossRef Google Scholar
Yoshinaga F, Tonouchi N, Watanabe K (1997) Research progress in production of bacterial cellulose by aeration and agitation culture and its application as a new industrial material. Biosci Biotechnol Biochem 61:219–224.
https://doi.org/10.1271/bbb.61.219 CrossRef Google Scholar
Zhu H, Jia S, Wan T, Jia Y, Yang H, Li J, Yan L, Zhong C (2011) Biosynthesis of spherical Fe
/bacterial cellulose nanocomposites as adsorbents for heavy metal ions. Carbohydr Polym 86:1558–1564.
https://doi.org/10.1016/j.carbpol.2011.06.061 CrossRef Google Scholar Copyright information
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