Preparation and characterization of magnetic Fe3O4–chitosan nanoparticles for cellulase immobilization
- 557 Downloads
Cellulase immobilization is an important issue for cellulose hydrolysis in bioethanol production. In this study, Fe3O4 nanoparticles were synthesized using a coprecipitation method and were coated with chitosan. Glutaraldehyde was used as cross-linking reagent for cellulase immobilization. The morphology, structure, and related physical and chemical properties of the supports were studied by X-ray diffraction, a physical property measurement system, Fourier transform infrared spectroscopy, and Transmission electron microscopy. Response surface methodology was used to determine the optimal cellulase loading efficiency (LE) and standard recovery ratio (RR). The verification tests show the immobilized particles had an LE of 99.6% and an RR value of 68.5%. It was found that immobilized cellulase had a wider range of adaptability to hydrolysis pH and temperature when compared with free cellulase. Five hydrolysis experiments demonstrated effective hydrolysis using immobilized cellulase while maintaining on average 80% of the free cellulase’s activity.
KeywordsMagnetic nanoparticles Immobilization Cellulase Performance optimization
The research project was sponsored by the Major Science and Technology Program for Water Pollution Control and Treatment (2009ZX07101-015-003). The authors would also like to thank Tyler Barzee at University of California, Davis for his invaluable support.
- Rahikainen JL, Martinsampedro R, Heikkinen H, Rovio S, Marjamaa K, Tamminen T, Rojas OJ, Kruus K (2013) Inhibitory effect of lignin during cellulose bioconversion: the effect of lignin chemistry on non-productive enzyme adsorption. Bioresour Technol 133(2):270–278. doi: 10.1016/j.biortech.2013.01.075 CrossRefPubMedGoogle Scholar
- Sánchez-Ramírez J, Martínez-Hernández JL, Segura- Ceniceros P, López G, Saade H, Medina-Morales MA, Ramos-González R, Aguilar CN, Ilyina A (2016) Cellulases immobilization on chitosan-coated magnetic nanoparticles: application for Agave atrovirens lignocellulosic biomass hydrolysis. Bioprocess Biosyst Eng 40:9–22. doi: 10.1007/s00449-016-1670-1 CrossRefPubMedGoogle Scholar
- Zhang J, Song Y, Wang B, Zhang X, Tan T (2016) Biomass to bio-ethanol: the evaluation of hybrid Pennisetum used as raw material for bio-ethanol production compared with corn stalk by steam explosion joint use of mild chemicals. Renew Energy 88:164–170. doi: 10.1016/j.renene.2015.11.034 CrossRefGoogle Scholar