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Cellulose

, Volume 24, Issue 12, pp 5541–5550 | Cite as

Preparation and characterization of magnetic Fe3O4–chitosan nanoparticles for cellulase immobilization

  • Yan Lin
  • Xi Liu
  • Zhaohui Xing
  • Yong Geng
  • Jeffrey Wilson
  • Deyi Wu
  • Hainan KongEmail author
Original Paper

Abstract

Cellulase immobilization is an important issue for cellulose hydrolysis in bioethanol production. In this study, Fe3O4 nanoparticles were synthesized using a coprecipitation method and were coated with chitosan. Glutaraldehyde was used as cross-linking reagent for cellulase immobilization. The morphology, structure, and related physical and chemical properties of the supports were studied by X-ray diffraction, a physical property measurement system, Fourier transform infrared spectroscopy, and Transmission electron microscopy. Response surface methodology was used to determine the optimal cellulase loading efficiency (LE) and standard recovery ratio (RR). The verification tests show the immobilized particles had an LE of 99.6% and an RR value of 68.5%. It was found that immobilized cellulase had a wider range of adaptability to hydrolysis pH and temperature when compared with free cellulase. Five hydrolysis experiments demonstrated effective hydrolysis using immobilized cellulase while maintaining on average 80% of the free cellulase’s activity.

Keywords

Magnetic nanoparticles Immobilization Cellulase Performance optimization 

Notes

Acknowledgments

The research project was sponsored by the Major Science and Technology Program for Water Pollution Control and Treatment (2009ZX07101-015-003). The authors would also like to thank Tyler Barzee at University of California, Davis for his invaluable support.

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Copyright information

© Springer Science+Business Media B.V. 2017

Authors and Affiliations

  • Yan Lin
    • 1
  • Xi Liu
    • 1
  • Zhaohui Xing
    • 1
  • Yong Geng
    • 1
  • Jeffrey Wilson
    • 1
  • Deyi Wu
    • 1
  • Hainan Kong
    • 1
    Email author
  1. 1.School of Environmental Science and EngineeringShanghai Jiao Tong UniversityShanghaiChina

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