, Volume 23, Issue 1, pp 929–940 | Cite as

The influences of enzymatic processing on physico-chemical and pigment dyeing characteristics of cotton fabrics

  • Longyun HaoEmail author
  • Rui Wang
  • Lun Wang
  • Kuanjun Fang
  • Jingquan Liu
  • Yajing Men
Original Paper


First, a crude cellulase was used to treat cotton fabrics to investigate its influences on the physicochemical properties of cotton. The FTIR and XRD analyses both confirmed the enzymatic treatment could increase the crystallinity of cotton, especially at a higher cellulase dosage. Once treated, the number of dissociable groups (–COOH) in cotton decreased, while that of the reducing groups (–CHO) increased. Second, copper phthalocyanine (CuPc) was selected to prepare an anionic nanoscale pigment dispersion to detect its dyeability on different cotton samples. It was concluded that the enzymatic hydrolysis itself had no significant impacts on the pigment dyeing performance. However, cellulase protein still stayed on the cotton surface after treatment and produced an enhancement effect on the pigment uptake due to strong hydrophobic interactions between them. This could be verified by K/S measurement and SEM observations.


Cellulase Cotton Copper phthalocyanine Nanoscale pigment Enhancement effect 



This work was financially supported by the National Natural Science Foundation of China (21303092), Science and Technology Plan of Qingdao (13-1-4-247-jch), Shandong Provincial Post-doctoral Foundation (127010) and Shandong Provincial Natural Science Foundation (ZR2010EQ 034).


  1. Adamczyk Z, Nattich M, Wasilewska M, Sadowska M (2011) Deposition of colloid particles on protein layers: fibrinogen on mica. J Colloid Interface Sci 356:454–464CrossRefGoogle Scholar
  2. Adeel S, Usman M, Haider W, Saeed M, Muneer M, Ali M (2015) Dyeing of gamma irradiated cotton using Direct Yellow 12 and Direct Yellow 27: improvement in colour strength and fastness properties. Cellulose 22:2095–2105CrossRefGoogle Scholar
  3. Akerholm M, Hinterstoisser B, Salmen L (2004) Characterization of the crystalline structure of cellulose using static and dynamic FT-IR spectroscopy. Carbohydr Res 339:569–578CrossRefGoogle Scholar
  4. Andreaus J, Azevedo H, Cavaco-Paulo A (1999) Effects of temperature on the cellulose binding ability of cellulase enzymes. J Mol Catal B Enzym 7:233–239CrossRefGoogle Scholar
  5. Cao Y, Tan H (2005) Study on crystal structures of enzyme-hydrolyzed cellulosic materials by X-ray diffraction. Enzyme Microb Technol 36:314–317CrossRefGoogle Scholar
  6. Cavaco-paulo A, Almeida L, Bishop D (1996) Effects of agitation and endoglucanase pretreatment on the hydrolysis of cotton fabrics by a total cellulase. Text Res J 5:287–294CrossRefGoogle Scholar
  7. Chung C, Lee M, Choe EK (2004) Characterization of cotton fabric scouring by FT-IR ATR spectroscopy. Carbohydr Polym 58:417–420CrossRefGoogle Scholar
  8. Cortez JM, Ellis J, Bishop DP (2002) Using cellulases to improve the dimensional stability of cellulosic fabrics. Text Res J 72:673–680CrossRefGoogle Scholar
  9. Csiszar E, Urbanszki K, Szakacs G (2001) Biotreatment of desized cotton fabric by commercial cellulase and xylanase enzymes. J Mol Catal B Enzym 11:1065–1072CrossRefGoogle Scholar
  10. Dong J, Chen S, Corti DS, Franses EI, Zhao Y, Ng HT, Hanson E (2011) Effect of Triton X-100 on the stability of aqueous dispersions of copper phthalocyanine pigment nanoparticles. J Colloid Interface Sci 362:33–41CrossRefGoogle Scholar
  11. Dourado F, Mota M, Pala H, Gama FM (1999) Effect of cellulase adsorption on he surface and interfacial properties of cellulose. Cellulose 6:265–282CrossRefGoogle Scholar
  12. Driemeier C, Calligaris GA (2011) Theoretical and experimental developments for accurate determination of crystallinity of celllose I materials. J Appl Crystallogr 44:184–192CrossRefGoogle Scholar
  13. Elazzouzi-Hafraoui S, Nishiyama Y, Putaux J, Heux L, Dubreuil F, Rochas C (2008) The shape and size distribution of crystalline nanoparticles prepared by acid hydrolysis of native cellulose. Biomacromolecules 9:57–65CrossRefGoogle Scholar
  14. French AD, Santiago Cintrón M (2013) Cellulose polymorphy, crystallite size, and the Segal crystallinity index. Cellulose 20:583–588CrossRefGoogle Scholar
  15. Gulrajani ML, Dayal A, Chakraborty M (1998) Kawabata evaluation of enzyme treated cotton knitted fabrics. Indian J Fibre Text 23:160–164Google Scholar
  16. Gusakov AV, Sinitsyn AP, Berlin AG, Markov AV, Ankudimova NV (2000) Surface hydrophobic amino acid residues in cellulase molecules as a structural factor responsible for their high denim-washing performance. Enzyme Microb Technol 27:664–671CrossRefGoogle Scholar
  17. Hao L, Cai Y, Fang K (2009) Dyeing of lyocell fabrics with pigment dispersion systems. J Disper Sci Technol 30:332–335CrossRefGoogle Scholar
  18. Hao L, Cai Y, Wang R (2011) Preparation of ultrafine pigment dispersion and investigation of its adsorption performance on cationized flax substrate. Adsorpt Sci Technol 29:875–885CrossRefGoogle Scholar
  19. Hao L, Wang R, Liu J, Cai Y, Liu R (2012a) Investigating the adsorption performance of nanoscale pigment on cationized cotton substrate. Powder Technol 222:176–181CrossRefGoogle Scholar
  20. Hao L, Wang R, Liu J, Liu R (2012b) The adsorptive and hydrolytic performance of cellulase on cationised cotton. Carbohydr Polym 89:171–176CrossRefGoogle Scholar
  21. Hao L, Wang R, Liu J, Liu R (2012c) Ultrasound-assisted adsorption of anionic nanoscale pigment on cationised cotton fabrics. Carbohydr Polym 90:1420–1427CrossRefGoogle Scholar
  22. Hao L et al (2014) Utilizing cellulase as a hydrogen peroxide stabilizer to combine the biopolishing and bleaching procedures of cotton cellulose in one bath. Cellulose 21:777–789CrossRefGoogle Scholar
  23. Hashem M, El-Bisi M, Sharaf S, Refaie R (2010) Pre-cationization of cotton fabrics: an effective alternative tool for activation of hydrogen peroxide bleaching process. Carbohydr Polym 79:533–540CrossRefGoogle Scholar
  24. Henrissat B (1994) Cellulases and their interaction with cellulose. Cellulose 1:169–196CrossRefGoogle Scholar
  25. Kang SY, Epps HH (2009) Effect of scouring and enzyme treatment on moisture regain percentage of naturally colored cottons. J Text I 100:598–606CrossRefGoogle Scholar
  26. Khan AA, Iqbal N, Adeel S, Azeem M, Batool F, Bhatti IA (2014) Extraction of natural dye from red calico leaves: gamma ray assisted improvements in colour strength and fastness. Dyes Pigments 103:50–54CrossRefGoogle Scholar
  27. Langan P, Nishiyama Y, Chanzy H (2001) X-ray structure of mercerized cellulose II at 1Å resolution. Biomacromolecules 2:410–416CrossRefGoogle Scholar
  28. Lenting HBM, Warmoeskerken MMCG (2001) Guidelines to come to minimized tensile strengh losss upon cellulase application. J Biotechnol 89:227–232CrossRefGoogle Scholar
  29. Lewin M, Ettinger A (1969) Oxidation of cellulose by hydrogen peroxide. Cell Chem Technol 3:9–20Google Scholar
  30. Li Y, Hardin IR (1997) Enzymatic scouring of cotton: effects on structure and properties. Text Chem Color 8:71–76Google Scholar
  31. Lv J, Zhou X (1999) Relationship between cellulase treatment and dyeing properties of cotton fabrics. J China Text Univ 16:32–34Google Scholar
  32. Mori R, Haga T, Takagishi T (1996) Reactive dye dyeability of cellulose fibers with cellulase treatment. J Appl Polym Sci 59:1263–1269CrossRefGoogle Scholar
  33. Nelson ML, O’Connor RT (1964) Relations of certain infrared bands to cellulose crystallinity and crystal lattic type. Part I. Spectra of lattice types I, II, III and amorphous cellulose. J Appl Polym Sci 8:1311–1324CrossRefGoogle Scholar
  34. Nishiyama Y (2009) Structure and properties of the cellulose microfibril. J Wood Sci 55:241–249CrossRefGoogle Scholar
  35. Nithya E, Radhai R, Rajendran R, Shalini S, Rajendran V, Jayakumar S (2011) Synergetic effect of DC air plasma and cellulase enzyme treatment on the hydrophilicity of cotton fabric. Carbohydr Polym 83:1652–1658CrossRefGoogle Scholar
  36. Ogeda TL, Silva IB, Fidale LC, Seoud OAE, Petri DFS (2012) Effect of cellulose physical characteristics, especially the water sorption value, on the efficiency of its hydrolysis catalyzed by free or immobilized cellulase. J Biotechnol 157:246–252CrossRefGoogle Scholar
  37. Pandiyaraj KN, Selvarajan V (2008) Non-thermal plasma treatment for hydrophilicity improvement of grey cotton fabrics. J Mater Process Technol 199:130–139CrossRefGoogle Scholar
  38. Paralikar KM, Bhatawdekar SP (1984) Hydrolysis of cotton fibres by cellulase enzymes. J Appl Polym Sci 29:2573–2580CrossRefGoogle Scholar
  39. Park S, Venditti RA, Abrecht DG, Jameel H, Pawlak JJ, Lee JM (2007) Surface and pore structure modificatellulose fibers through cellulase treatment. J Appl Polym Sci 103:3833–3839CrossRefGoogle Scholar
  40. Park S, Baker JO, Himmel ME, Parilla PA, Johnson DK (2010) Cellulose crystallinity index: measurement techniques and their impact on interpreting cellulase performance. Biotechnol Biofuels 3:1–10CrossRefGoogle Scholar
  41. Paulo AC, Almeida L (1996) Effect of agitation and endoglucanase pretreatment on the hydrolysis of cotton fabrics by a total cellulase. Text Res J 66:287–294CrossRefGoogle Scholar
  42. Rousselle MA, Howley PS (1998) Molecular weight of cotton cellulose: effect of treatment with a total cellulase. Text Res J 68:606–610CrossRefGoogle Scholar
  43. Saravanan D, Vasanthi NS, Ramachandran T (2009) A review on influential behaviour of biopolishing on dyeability and certain physico-mechanical properties of cotton fabrics. Carbohydr Polym 76:1–7CrossRefGoogle Scholar
  44. Segal L, Creely JJ Jr, Martin AE, Conrad CM (1959) An empirical method for estimating the degree of crystallinity of native cellulose using the X-ray diffractometer. Text Res J 29:786–794CrossRefGoogle Scholar
  45. Spinelli HJ (1998) Polymeric dispersants in ink jet technology. Adv Mater 10:1215–1218CrossRefGoogle Scholar
  46. Sugiyama J, Persson J, Chanzy H (1991) Combined infrared and electron diffraction study of the polymorphism of native celluloses. Macromolecules 24:2461–2466CrossRefGoogle Scholar
  47. Tsatsaroni E, Liakopoulou-Kyriakides M (1995) Effect of enzymatic treatment on the dyeing of cotton and wool fibres with natural dyes. Dyes Pigments 29:203–209CrossRefGoogle Scholar
  48. Wada M, Okano T, Sugiyama J (1997) Sychrotron-radiaated X-ray and neutron diffraction study of native cellulose. Cellulose 4:221–232CrossRefGoogle Scholar
  49. Wang N, Zha A, Wang J (2008) Study on the wicking property of polyester filament yarns. Fiber Polym 9:97–100CrossRefGoogle Scholar
  50. Xie K, Hu C, Zhang X (2012) Low temperature bleaching and dyeing properties of modified cellulose fabrics with triazine derivative. Carbohydr Polym 87:1756–1762CrossRefGoogle Scholar
  51. Yamade M, Amano Y, Horikawa E, Nozaki K, Kanda T (2005) Mode of action of cellulase on dyed cotton with a reactive dye. Biosci Biotech Bioch 69:45–50CrossRefGoogle Scholar
  52. Yue Y, Zhou C, French AD, Xia G, Han G, Wang Q, Wu Q (2012) Comparative properties of cellulose nano-crystals from native and mercerized cotton fibers. Cellulose 19:1173–1187CrossRefGoogle Scholar
  53. Zemljic LF (2008) Carboxyl groups in pre-treated regenerated cellulose fibres. Cellulose 15:681–690CrossRefGoogle Scholar
  54. Zhang M et al (2011) Highly efficient decomposition of organic dye by aqueous-solid phase transfer and in situ photocatalysis using hierarchical copper phthalocyanine hollow spheres. ACS Appl Mater Inter 3:2573–2578CrossRefGoogle Scholar
  55. Zolriasatein AA, Yazdanshenas ME (2014) Changes in composition, appearence, physical, and dyeing properties of jute yarn after bio-pretreatment with laccase, xylanase, cellulase, and pectinase enzymes. J Text I 105:609–619CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media Dordrecht 2015

Authors and Affiliations

  • Longyun Hao
    • 1
    • 2
    • 3
    Email author
  • Rui Wang
    • 1
  • Lun Wang
    • 4
  • Kuanjun Fang
    • 1
    • 2
  • Jingquan Liu
    • 1
  • Yajing Men
    • 3
  1. 1.Chemical Engineering College of Qingdao UniversityQingdaoChina
  2. 2.Collaborative Innovation Center for Marine Biomass Fibers, Materials and Textiles of Shandong ProvinceQingdaoChina
  3. 3.Sunvim Group Co., Ltd.GaomiChina
  4. 4.Exit-Entry Inspection and Quarantine Bureau of QingdaoQingdaoChina

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