, Volume 22, Issue 5, pp 3359–3375 | Cite as

Isolation of phenolic compounds from larch wood waste using pressurized hot water: extraction, analysis and economic evaluation

  • Matej Ravber
  • Željko Knez
  • Mojca Škerget
Original Paper


The main aim of this study was to evaluate pressurized hot water as a green and environmentally friendly extraction medium for the isolation of phenolic compounds from larch waste wood. Such isolates could find applications in the food, feed, pharmaceutical and cosmetics industries or as natural ingredients for adhesives or biocidal coatings in the wood industry. In the first step different larch wood fractions were extracted using batch system aiming to determine the most suitable fraction for isolation. The content of extractives, total phenolic and tannin content of obtained extracts and their antioxidant activity were evaluated. Secondly, semi-continuous operation was applied, where effects of temperature, addition of ethanol and flow rate were studied. Extraction yield was monitored and extracts were again analyzed for their total phenolic content and antioxidant activity. HPLC analysis was performed, in order to study the effect of temperature and addition of ethanol on the hydrothermal degradation of phenolics during semi-continuous operation. Finally, the economics of pilot-scale and industrial-scale processes with different extractor capacities, ranging from approximately 12 to 1200 kg of woody material per day and operating at optimal conditions for isolation of larch wood extractives was evaluated, in order to determine the cost of manufacturing of such a product.

Graphical Abstract


Pressurized hot water extraction Larch wood waste Phenolic compounds Hydrothermal degradation Economic evaluation 



Authors are grateful to the Slovenian Ministry of High Education, Science and Technology for the financial support of this work. This paper was produced within the framework of the operation entitled “Centre of Open innovation and ResEarch of University of Maribor (CORE@UM)”.

Supplementary material

10570_2015_719_MOESM1_ESM.rar (125 kb)
Supplementary material 1 (RAR 125 kb)


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Copyright information

© Springer Science+Business Media Dordrecht 2015

Authors and Affiliations

  1. 1.Faculty of Chemistry and Chemical EngineeringUniversity of MariborMariborSlovenia

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