Cellulose

, Volume 21, Issue 2, pp 1075–1086 | Cite as

Cellulose microfibril orientation in onion (Allium cepa L.) epidermis studied by atomic force microscopy (AFM) and vibrational sum frequency generation (SFG) spectroscopy

  • Kabindra Kafle
  • Xiaoning Xi
  • Christopher M. Lee
  • Bernhard R. Tittmann
  • Daniel J. Cosgrove
  • Yong Bum Park
  • Seong H. Kim
Original Paper

Abstract

Cellulose microfibril orientation in plant cell walls changes during cell expansion and development. The cellulose microfibril orientation in the abaxial epidermis of onion scales was studied by atomic force microscopy (AFM) and sum frequency generation (SFG) vibrational spectroscopy. Onion epidermal cells in all scales are elongated along the onion bulb axis. AFM images showed that cellulose microfibrils exposed at the innermost surface of the abaxial epidermis are oriented perpendicular to the bulb axis in the outer scales and more dispersed in the inner scales of onion bulb. SFG analyses can determine the orientation of cellulose microfibrils averaged over the entire thickness of the cell wall. We found that the average orientation of cellulose microfibrils inside onion abaxial epidermal cell walls as revealed by SFG is similar to the orientation observed at the innermost cell wall surface by AFM. The capability to determine the average orientation of cellulose microfibrils in intact cell walls will be useful to study how cellulose microfibril orientation is related to biomechanical properties and the growth mechanism of plant cell walls.

Keywords

Onion epidermis Cellulose microfibril Microfibril orientation Sum frequency generation spectroscopy Atomic force microscopy 

Supplementary material

10570_2013_121_MOESM1_ESM.doc (1.7 mb)
Supplementary material 1 (DOC 1767 kb)

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Copyright information

© Springer Science+Business Media Dordrecht 2013

Authors and Affiliations

  • Kabindra Kafle
    • 1
  • Xiaoning Xi
    • 2
  • Christopher M. Lee
    • 1
  • Bernhard R. Tittmann
    • 2
  • Daniel J. Cosgrove
    • 3
  • Yong Bum Park
    • 3
  • Seong H. Kim
    • 1
  1. 1.Department of Chemical Engineering and Materials Research InstituteThe Pennsylvania State UniversityUniversity ParkUSA
  2. 2.Department of Engineering Science and MechanicsThe Pennsylvania State UniversityUniversity ParkUSA
  3. 3.208 Mueller Laboratory, Department of BiologyThe Pennsylvania State UniversityUniversity ParkUSA

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