Cellulose

, Volume 18, Issue 6, pp 1433–1440 | Cite as

Anisotropy of cell wall polymers in branches of hardwood and softwood: a polarized FTIR study

Article

Abstract

The mechanical and physical properties of wood fibres are dependent on the organisation of their constituent polymers (cellulose, hemicellulose and lignin). Fourier Transform Infrared (FTIR) microscopy was used to examine the anisotropy of the main wood polymers in isolated cell wall fragments from branches of maple and Serbian spruce. Polarised FTIR measurements indicated an anisotropy, i.e. orientation of the cellulose microfibrils that was more or less parallel to the longitudinal axis of the cell wall. The hemicelluloses, glucomannan and xylan appeared to have a close link to the orientation of the cellulose and, thus, an orientation more parallel to the axis of the cell wall. An important result is that, in both maple and spruce samples, lignin was found to be organised in a parallel way in relation to the longitudinal cell wall axis, as well as to the cellulose. The results show that, despite the different lignin precursors and the different types of hemicelluloses in these two kinds of wood, lignin has a similar orientation, when it comes to the longitudinal axis of the cell wall.

Keywords

Cellulose Glucomannan Lignin Orientation Wood Xylan 

Supplementary material

10570_2011_9584_MOESM1_ESM.doc (369 kb)
Supplementary material 1 (DOC 367 kb)

References

  1. Åkerholm M, Salmén L (2001) Interactions between wood polymers studied by dynamic FT-IR spectroscopy. Polymer 42:963–969CrossRefGoogle Scholar
  2. Åkerholm M, Hinterstoisser B, Salmén L (2004) Characterization of the crystalline structure of cellulose using statistic and dynamic FT-IR spectroscopy. Carbohydr Res 339:569–578CrossRefGoogle Scholar
  3. Aspinall GO (1980) Chemistry of cell wall polysaccharides. In: Priess J (ed) The biochemistry of plants: a comprehensive treatise. Academic Press, New York, pp 477–500Google Scholar
  4. Atalla RH, Agarwal UP (1985) Raman microprobe evidence for lignin orientation in the cell walls of native woody tissue. Science 227:636–638CrossRefGoogle Scholar
  5. Baskin TI (2005) Anisotropic expansion of the plant cell wall. Annu Rev Cell Dev Biol 21:203–222CrossRefGoogle Scholar
  6. Bergander A, Salmén L (2002) Cell wall properties and their effects on the mechanical properties of fibres. J Mater Sci 37:151–156CrossRefGoogle Scholar
  7. Bogdanovic J, Djikanovic D, Maksimovic V, Tufegdzic S, Djokovic D, Isajev V, Radotic K (2006) Phenolics, lignin content and peroxidase activity in Picea omorika lines. Biol Plant 50:461–464CrossRefGoogle Scholar
  8. Bogdanovic J, Milosavic N, Prodanovic R, Ducic T, Radotic K (2007) Variability of antioxidant enzyme activity and isoenzyme profile in needles of Serbian spruce (Picea omorika (Panc.) Purkinye). Biochem System Ecol 35:263–273CrossRefGoogle Scholar
  9. Chen M, Sommer AJ, McClure JW (2000) Fourier transform–IR determination of protein contamination in thioglycolic acid lignin from radish seedlings and improved methods for extractive-free cell wall preparation. Phytochem Anal 11:153–159CrossRefGoogle Scholar
  10. Faix O (1991) Classification of lignins from different botanical origins by FTIR spectroscopy. Holzforschung 45:21–27CrossRefGoogle Scholar
  11. Gierlinger N, Goswami L, Schmidt M, Burgert I, Coutand C, Rogge T, Schwanninger M (2008) In situ FT-IR microscopic study on enzymatic treatment of poplar wood cross-sections. Biomacromolecules 9:2194–2201CrossRefGoogle Scholar
  12. Harris PJ (1983) Cell walls. In: Hall JL, Moore AL (eds) Isolation of membranes and organelles from plant cells. Academic Press, London, pp 25–53Google Scholar
  13. Hatfield R, Vermeris W (2001) Lignin formation in plants. The dilemma of linkage specificity. Plant Physiol 126:1351–1357CrossRefGoogle Scholar
  14. Houtman CJ, Atalla RH (1995) Cellulose: lignin interactions. A computational study. Plant Physiol 107:977–984Google Scholar
  15. Liang CY, Basset KH, McGinnes EA, Marchessault RH (1960) Infrared spectra of crystalline polysaccharides; VII. Thin wood sections. Tappi 43:232–235Google Scholar
  16. Marchessault RH (1962) Application of infra-red spectroscopy to cellulose and woody polysaccharides. Pure Appl Chem 5:107–129CrossRefGoogle Scholar
  17. Melton LD, Smith BG (2001) Current protocols in food analytical chemistry. Wiley, New YorkGoogle Scholar
  18. Micic M, Jeremic M, Radotic K, Mavers M, Leblanc R (2000) Visualization of artificial lignin supramolecular structures. Scanning 22:288–294CrossRefGoogle Scholar
  19. Micic M, Orbulescu J, Radotic K, Jeremic M, Sui G, Zheng Yu, Leblanc RM (2002) ZL-DHP lignin model compound at the air–water interface. Biophys Chem 99:55–62CrossRefGoogle Scholar
  20. Micic M, Radotic K, Jeremic M, Leblanc RM (2003) Study of self-assembly of the lignin model compound on cellulose model substrate. Macromol Biosci 3:100–106CrossRefGoogle Scholar
  21. Monties B (1998) Novel structures and properties of lignins in relation to their natural and induced variability in ecotypes, mutants and transgenic plants. Polym Degrad Stabil 59:53–64CrossRefGoogle Scholar
  22. Olsson A-M, Bjurhager I, Gerber L, Sundberg B, Salmén L (2011) Ultra-structural organisation of cell wall polymers in normal- and tension wood of aspen revealed by polarisation FT-IR microscopy. Planta 233:1277–1286Google Scholar
  23. Radotic K, Simic-Krstic J, Jeremic M, Trifunovic M (1994) A study of lignin formation at the molecular level by scanning tunneling microscopy. Biophys J 66:1763–1767CrossRefGoogle Scholar
  24. Salmon S, Hudson SM (1997) Crystal morphology, biosynthesis and physical assembly of cellulose chitin and chitosan. J Macromol Sci C37:199–263Google Scholar
  25. Sarkanen KV, Hergert HL (1971) Classification and distribution. In: Sarkanen KV, Ludwig CH (eds) Lignins: occurrence, formation, structure and reactions. Wiley, New York, pp 43–94Google Scholar
  26. Steinbach G, Pomozi I, Zsiros O, Páy A, Horváth GV, Garab G (2008) Imaging fluorescence detected linear dichroism of plant cell walls in laser scanning confocal microscope. Cytometry 73A:202–208CrossRefGoogle Scholar
  27. Stevanic J, Salmén L (2009) Orientation of the wood polymers in the cell wall of spruce wood fibres. Holzforschung 63:497–503CrossRefGoogle Scholar
  28. Strack D, Heilemann J, Wray V, Dirks H (1988) Cell wall: conjugated phenolics from coniferae leaves. Phytochemistry 28:2071–2078CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media B.V. 2011

Authors and Affiliations

  1. 1.Institute for Multidisciplinary ResearchBelgradeSerbia
  2. 2.InnventiaStockholmSweden

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