Cellulose

, Volume 18, Issue 3, pp 739–747 | Cite as

Biochemical localization of a protein involved in synthesis of Gluconacetobacter hansenii cellulose

  • Prashanti R. Iyer
  • Jeffrey Catchmark
  • Nicole R. Brown
  • Ming Tien
Article

Abstract

Using subcellular fractionation and Western blot methods, we have shown that AcsD, one of the proteins encoded by the Acetobacter cellulose synthase (acs) operon, is localized in the periplasmic region of the cell. AcsD protein was heterologously expressed in Escherichia coli and purified using histidine tag affinity methods. The purified protein was used to obtain rabbit polyclonal antibodies. The purity of the subcellular fractions was assessed by marker enzyme assays.

Keywords

AcsD Gluconacetobacter Cellulose synthesis Periplasm 

Abbreviations

SDS-PAGE

Sodium dodecyl sulfate polyacrylamide gel electrophoresis

IPTG

Isopropyl thio-galactopyranoside

AcsD

Acetobacter cellulose synthase operon protein D

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Copyright information

© Springer Science+Business Media B.V. 2011

Authors and Affiliations

  • Prashanti R. Iyer
    • 1
  • Jeffrey Catchmark
    • 2
  • Nicole R. Brown
    • 3
  • Ming Tien
    • 4
  1. 1.Graduate Program in Chemical Biology, Huck Institute of Life SciencesThe Pennsylvania State UniversityUniversity ParkUSA
  2. 2.Department of Agricultural and Biological EngineeringThe Pennsylvania State UniversityUniversity ParkUSA
  3. 3.School of Forest ResourcesThe Pennsylvania State UniversityUniversity ParkUSA
  4. 4.Department of Biochemistry and Molecular BiologyThe Pennsylvania State UniversityUniversity ParkUSA

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