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Cellulose

, Volume 16, Issue 6, pp 1089–1097 | Cite as

Analysis of galactoglucomannans from spruce wood by capillary electrophoresis

  • Aleš DoliškaEmail author
  • Simona Strnad
  • Volker Ribitsch
  • Karin Stana Kleinschek
  • Stefan Willför
  • Bodo Saake
Article

Abstract

The aim of this study was to setup a method for detection and quantification of monosaccharide components in technical galactoglucomannas (T-GGM) from spruce wood using capillary zone electrophoresis (CZE). CZE technique was optimised regarding borate buffer concentrations, EOF modifier application, and system pH. Aqueous solution of T-GGM was chemically hydrolysed by sulphuric acid, in an autoclave. In this way obtained monosaccharides were derivatized with 4-amino benzoic acid ethyl ester via reductive amination using sodium cyanoborohydride. The results of the optimisation procedure showed that the borate buffers at lowest concentrations (100 and 200 mM) with acetonitrile addition as EOF modifier gave the optimal measurement results, as it showed sufficient separation at relatively short migration times. The amounts of single monosaccharide components in the T-GGM samples obtained by the optimised CZE procedure were practically the same in comparison to the results of the well established HPLC-anion exchange chromatography. On the basis of this research, it was concluded that the capillary zone electrophoresis is an efficient analytical procedure for the characterisation of galactoglucomannans derived from softwoods.

Keywords

Capillary electrophoresis Carbohydrates Galactoglucomannan Glucomannan Polysaccharide 

Abbreviations

ABEE

4-Amino benzoic acid ethyl ester

BGE

Background electrolyte

EOF

Electroosmotic flow

CZE

Capillary zone lectrophoresis

Gal

Galactose

GGM

Galactoglucomannans

Glc

Glucose

GM

Glucomannans

Man

Mannose

T-GGM

Technical galactoglucomannans

TMP

Thermomechanical pulp

SDS

Sodium dodecyl sulphate

Notes

Acknowledgments

This work is the part of EPNOE (European Polysaccharide Network of Excellence) activity.

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Copyright information

© Springer Science+Business Media B.V. 2009

Authors and Affiliations

  • Aleš Doliška
    • 1
    Email author
  • Simona Strnad
    • 1
  • Volker Ribitsch
    • 2
  • Karin Stana Kleinschek
    • 1
  • Stefan Willför
    • 3
  • Bodo Saake
    • 4
  1. 1.Faculty of Mechanical Engineering, Institute for Engineering Materials and DesignUniversity of MariborMariborSlovenia
  2. 2.Institute for ChemistryUniversity of GrazGrazAustria
  3. 3.Process Chemistry Centre, Laboratory of Wood and Paper ChemistryÅbo Akademi UniversityTurkuFinland
  4. 4.vTI-Institute for Wood Technology and Wood BiologyUniversity of HamburgHamburgGermany

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