, Volume 16, Issue 6, pp 1089–1097 | Cite as

Analysis of galactoglucomannans from spruce wood by capillary electrophoresis

  • Aleš DoliškaEmail author
  • Simona Strnad
  • Volker Ribitsch
  • Karin Stana Kleinschek
  • Stefan Willför
  • Bodo Saake


The aim of this study was to setup a method for detection and quantification of monosaccharide components in technical galactoglucomannas (T-GGM) from spruce wood using capillary zone electrophoresis (CZE). CZE technique was optimised regarding borate buffer concentrations, EOF modifier application, and system pH. Aqueous solution of T-GGM was chemically hydrolysed by sulphuric acid, in an autoclave. In this way obtained monosaccharides were derivatized with 4-amino benzoic acid ethyl ester via reductive amination using sodium cyanoborohydride. The results of the optimisation procedure showed that the borate buffers at lowest concentrations (100 and 200 mM) with acetonitrile addition as EOF modifier gave the optimal measurement results, as it showed sufficient separation at relatively short migration times. The amounts of single monosaccharide components in the T-GGM samples obtained by the optimised CZE procedure were practically the same in comparison to the results of the well established HPLC-anion exchange chromatography. On the basis of this research, it was concluded that the capillary zone electrophoresis is an efficient analytical procedure for the characterisation of galactoglucomannans derived from softwoods.


Capillary electrophoresis Carbohydrates Galactoglucomannan Glucomannan Polysaccharide 



4-Amino benzoic acid ethyl ester


Background electrolyte


Electroosmotic flow


Capillary zone lectrophoresis












Technical galactoglucomannans


Thermomechanical pulp


Sodium dodecyl sulphate



This work is the part of EPNOE (European Polysaccharide Network of Excellence) activity.


  1. Dahlaman O et al (2000) Analysis of carbohydrates in wood and pulps employing enzymatic hydrolysis and subsequent capillary zone electrophoresis. J Chromatogr A 891:157–174. doi: CrossRefGoogle Scholar
  2. Hannuksela T, Holmbom B (2004) Sorbtion of mannans to different fiber surfaces: an evolution of understanding, American Chemical SocietyGoogle Scholar
  3. Heinze T (2005) Polysaccharides I, structure, characterization and use, advances in polymer science. Springer, BerlinGoogle Scholar
  4. Honda S (1996) Review: separation of neutral carbohydrates by capillary electrophoresis. J Chromatogr A 720:337–351. doi: CrossRefGoogle Scholar
  5. Kazmaier T, Roth S, Zapp J, Harding M, Kuhn R (1998) Quantitative analysis of malto-oligosaccharides by MALDI-TOF mass spectrometry, capillary electrophoresis and anion exchange chromatography. Fresenius J Anal Chem 361:473–478. doi: CrossRefGoogle Scholar
  6. Lagane B, Treilhou M, Couderc F (2000) Capillary electrophoresis: theory, teaching approach and separation of oligosaccharides using indirect UV detection. Biochem Mol Biol Educ 28(5):251–255Google Scholar
  7. Lamari FN et al (2003) Derivatization of carbohydrates for chromatographic, electrophoretic and masss spectrometric structure analysis. J Chromatogr B 793:15–36. doi: CrossRefGoogle Scholar
  8. Nguyen DT, Lerch H, Zemann A, Bonn G (1997) Separation of derivatized carbohydrates by co-electroosmotic capillary electrophoresis. Chromatographia 46(3–4):113–121. doi: CrossRefGoogle Scholar
  9. Pulus A, Klockow A (1996) Review: detection of carbohydrates in capillary electrophoresis. J Chromatogr A 720:353–376. doi: CrossRefGoogle Scholar
  10. Ristolainen M (1999) Characterization of totally chlorine-free effluents from Kaft pulp bleaching II. Analysis of carbohydrate-derived constituents after acid hydrolysis by CZE. J Chromatogr A 832:203–209. doi: CrossRefGoogle Scholar
  11. Ristolainen M, Alén R (2001) Carbohydrates from chemical pulps: characterization by capillary zone electrophoresis. Encyclopedia of Analytical Chemistry: Applications, Theory, and Instrumentation, pp 8345–8361Google Scholar
  12. Rydlund A, Dahlman O (1996) Efficient capillary zone electrophoretic separation of wood-derived neutral and acidic mono- and oligosaccharides. J Chromatogr A 738(1):129–140. doi: CrossRefGoogle Scholar
  13. Rydlund A, Dahlman O (1997) Oligosaccharides obtained by enzymatic hydrolysis of birch kraft pulp xylan: analysis by capillary zone electrophoresis and mass spectrometry. Carbohydr Res 300:95–102CrossRefGoogle Scholar
  14. Santos SM et al (2007) Development and application of capillary electrophoresis based method for the assessment of monosaccharide in soil using acid hydrolysis. Talanta 72:165–171. doi: CrossRefGoogle Scholar
  15. Sjöström E (1993) Wood chemistry-fundamentals and applications, 2nd edn. Academic Press, New YorkGoogle Scholar
  16. Sundberg K et al (1996) Determination of hemicelluloses and pectincs in wood and pulp fibres by acid methanolysis and gas chromatography. Nordic Pulp Pap Res 11(4):216–219. doi: CrossRefGoogle Scholar
  17. Willför S, Sjöholm R, Laine C, Roslund M, Hemming J, Holmbom B (2003) Characterisation of water-soluble galactoglucomannans from Norway spruce wood and thermomechanical pulp. Carbohydr Polym 52:175–187. doi: CrossRefGoogle Scholar
  18. Willför S, Sundberg K, Tenkanen M, Holmbom B (2008) Spruce-derived mannan-A potential raw material for hydrocolloids and novel advanced natural materials. Carbohydr Polym 72:197–210. doi: CrossRefGoogle Scholar
  19. Woodward C, Weinberger R (1994) Capillary zone electrophoresis of carbohydrates derivatized with 4-aminobenzoic acid ethyl ester. Aplication Note, Hewlett PackardGoogle Scholar

Copyright information

© Springer Science+Business Media B.V. 2009

Authors and Affiliations

  • Aleš Doliška
    • 1
    Email author
  • Simona Strnad
    • 1
  • Volker Ribitsch
    • 2
  • Karin Stana Kleinschek
    • 1
  • Stefan Willför
    • 3
  • Bodo Saake
    • 4
  1. 1.Faculty of Mechanical Engineering, Institute for Engineering Materials and DesignUniversity of MariborMariborSlovenia
  2. 2.Institute for ChemistryUniversity of GrazGrazAustria
  3. 3.Process Chemistry Centre, Laboratory of Wood and Paper ChemistryÅbo Akademi UniversityTurkuFinland
  4. 4.vTI-Institute for Wood Technology and Wood BiologyUniversity of HamburgHamburgGermany

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