, Volume 13, Issue 6, pp 689–696 | Cite as

Protease and ROS activities influenced by a composite of bacterial cellulose and collagen type I in vitro

  • Cornelia Wiegand
  • Peter Elsner
  • Uta-Christina Hipler
  • Dieter Klemm


The application of bacterial cellulose as a dressing for chronic and burn wounds has been described as beneficial for healing. However, this study and other tests have shown that bacterial cellulose has no significant influence on the biochemical state of chronic wounds. Such wounds persist in the inflammatory phase of the normal healing process and fail to heal. Exudates from non-healing wounds show elevated levels of proteolytic enzymes, cytokines and reactive oxygen species (ROS), leading to a reduced concentration of growth factors and proteinase inhibitors and to a degradation of tissue and severe damage. Thus, the reduction of protein degrading enzymes and proinflammatory interleukins as well as scavenging of ROS appears to be a suitable way to support the healing process. The aim of the present study was to improve the positive features of bacterial cellulose as wound dressing by incorporation of collagen type I into the cellulose pellicle. The research focussed on the possible in situ formation of a composite of bacterial cellulose and collagen type I. This biomaterial is able to reduce the amount of selected proteases and interleukins significantly and possesses a distinct antioxidant capacity as well.


Bacterial cellulose Collagen Gluconacetobacter xylinus Matrix metalloprotease PMN elastase Reactive oxygen species 



matrix metalloproteases


oxidized regenerated cellulose

PMN granulocytes

polymorphonuclear granulocytes


reactive oxygen species


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Copyright information

© Springer 2006

Authors and Affiliations

  • Cornelia Wiegand
    • 1
  • Peter Elsner
    • 1
  • Uta-Christina Hipler
    • 1
  • Dieter Klemm
    • 1
  1. 1.Department of DermatologyFriedrich Schiller UniversityJenaGermany

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