The effects of five types of tea solutions on epiboly process, neural and cardiovascular development, and locomotor capacity of zebrafish

  • Xu Li
  • Qiuping Zhang
  • Xi Peng
  • Jia Xu
  • Yuan Zhang
  • Jiaojiao Zhu
  • Yuhua WangEmail author
  • Yunhe AnEmail author
  • Dongyan ChenEmail author
Original Article


The effects of teas on embryonic development are still known little. The objective of this study was to compare and analyze developmental effects of green tea, delicate flavor oolong tea, strong flavor oolong tea, black tea, and pu’er tea using zebrafish embryos. Embryos were exposed in tea solutions from one-cell stage; the morphology, locomotor capacity, and gene expression of embryos or larvae were analyzed. The results showed that either tea could decrease the length of body and the size of head and eyes. The effect of green tea had the most significant effects on morphology. Only green tea disturbed cell movement, epiboly, and nervous system development. All five tea solutions caused heart structure alternations and lowered heart rates, and effects caused by green tea were severe. Green tea inhibited the formation of dorsal aorta and segmental arteries and decreased the velocity and total movement distance of larvae. In conclusion, the toxicity of green tea to epiboly, neural and cardiovascular development, and locomotor capacity is more severe than that of other teas. Our study played a warning role for safety consumption of teas and provided references for further study of tea’s physiological and pharmacological effects and biological activity.


Cardiovascular Developmental toxicity Locomotor capacity Nervous Tea Zebrafish 


Funding information

This research was funded under the Special Fund for Basic Research on Scientific Instruments from the Chinese National Natural Science Foundation (grant no. 61327802) and the Chinese National Natural Science Foundation of China (Grant No. 31770733).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest

Supplementary material

10565_2018_9453_MOESM1_ESM.docx (1.9 mb)
ESM 1 (DOCX 1987 kb)


  1. Arab L, Khan F, Lam H. Tea consumption and cardiovascular disease risk. Am J Clin Nutr. 2013;98(6 Suppl):1651S–9S.CrossRefGoogle Scholar
  2. Balentine DA, Wiseman SA, Bouwens LC. The chemistry of tea flavonoids. Crit Rev Food Sci Nutr. 1997;37(8):693–704.CrossRefGoogle Scholar
  3. Barenys M, Gassmann K, Baksmeier C, Heinz S, Reverte I, Schmuck M, et al. Epigallocatechin gallate (EGCG) inhibits adhesion and migration of neural progenitor cells in vitro. Arch Toxicol. 2016;91(2):1–11.Google Scholar
  4. Bedrood Z, Rameshrad M, Hosseinzadeh H. Toxicological effects of Camellia sinensis (green tea): a review. Phytother Res. 2018;32(7):1163–80.CrossRefGoogle Scholar
  5. Benini A, Cignarella F, Calvarini L, Mantovanelli S, Giacopuzzi E, Zizioli D, et al. slc7a6os gene plays a critical role in defined areas of the developing CNS in zebrafish. Plos One. 2015;10(3):e0119696.CrossRefGoogle Scholar
  6. Borday C, Chatonnet F, Thoby-Brisson M, Champagnat J, Fortin G. Neural tube patterning by Krox20 and emergence of a respiratory control. Respir Physiol Neurobiol. 2005;149(1–3):63–72.CrossRefGoogle Scholar
  7. Fujiki H, Suganuma M. Green tea: an effective synergist with anticancer drugs for tertiary cancer prevention. Cancer Lett. 2012;324(2):119–25.CrossRefGoogle Scholar
  8. Grove KA, Lambert JD. Laboratory, epidemiological, and human intervention studies show that tea (Camellia sinensis) may be useful in the prevention of obesity. J Nutr. 2010;140(3):446–53.CrossRefGoogle Scholar
  9. Heber D, Zhang Y, Yang J, Ma JE, Henning SM, Li Z. Green tea, black tea, and oolong tea polyphenols reduce visceral fat and inflammation in mice fed high-fat, high-sucrose obesogenic diets. J Nutr. 2014;144(9):1385–93.CrossRefGoogle Scholar
  10. Hodgson JM, Croft KD. Tea flavonoids and cardiovascular health. Mol Asp Med. 2010;31(6):495–502.CrossRefGoogle Scholar
  11. Jeng KC, Chen CS, Fang YP, Hou RC, Chen YS. Effect of microbial fermentation on content of statin, GABA, and polyphenols in Pu-Erh tea. J Agric Food Chem. 2007;55(21):8787–92.CrossRefGoogle Scholar
  12. Kalueff AV, Stewart AM, Gerlai R. Zebrafish as an emerging model for studying complex brain disorders. Trends Pharmacol Sci. 2014;35(2):63–75.CrossRefGoogle Scholar
  13. Kane DA, Hammerschmidt M, Mullins MC, Maischein HM, Brand M, van Eeden FJ, et al. The zebrafish epiboly mutants. Development. 1996;123:47–55.Google Scholar
  14. Karlstrom RO, Tyurina OV, Kawakami A, Nishioka N, Talbot WS, Sasaki H, et al. Genetic analysis of zebrafish gli1 and gli2 reveals divergent requirements for gli genes in vertebrate development. Development. 2003;130(8):1549–64.CrossRefGoogle Scholar
  15. Kondo T, Ohta T, Igura K, Hara Y, Kaji K. Tea catechins inhibit angiogenesis in vitro, measured by human endothelial cell growth, migration and tube formation, through inhibition of VEGF receptor binding. Cancer Lett. 2002;180(2):139–44.CrossRefGoogle Scholar
  16. Krauss S, Johansen T, Korzh V, Fjose A. Expression of the zebrafish paired box gene pax [zf-b] during early neurogenesis. Development. 1991;113(4):1193–206.Google Scholar
  17. Kris-Etherton PM, Keen CL. Evidence that the antioxidant flavonoids in tea and cocoa are beneficial for cardiovascular health. Curr Opin Lipidol. 2002;13(1):41–9.CrossRefGoogle Scholar
  18. Li Q, Zhao HF, Zhang ZF, Liu ZG, Pei XR, Wang JB, et al. Long-term green tea catechin administration prevents spatial learning and memory impairment in senescence-accelerated mouse prone-8 mice by decreasing Abeta1-42 oligomers and upregulating synaptic plasticity-related proteins in the hippocampus. Neuroscience. 2009;163(3):741–9.CrossRefGoogle Scholar
  19. Lin JK, Lin CL, Liang YC, Linshiau S, Juan IM. Survey of catechins, gallic acid, and methylxanthines in green, oolong, pu-erh, and black teas. J Agric Food Chem. 1998;46(9):3635–42.CrossRefGoogle Scholar
  20. Lo HM, Hung CF, Huang YY, Wu WB. Tea polyphenols inhibit rat vascular smooth muscle cell adhesion and migration on collagen and laminin via interference with cell-ECM interaction. J Biomed Sci. 2007;14(5):637–45.CrossRefGoogle Scholar
  21. Marlow F, Gonzalez EM, Yin C, Rojo C, Solnica-Krezel L. No tail co-operates with non-canonical Wnt signaling to regulate posterior body morphogenesis in zebrafish. Development. 2004;131(1):203–16.CrossRefGoogle Scholar
  22. Namazi Shabestari A, Saeedi Moghaddam S, Sharifi F, Fadayevatan R, Nabavizadeh F, Delavari A, et al. The most prevalent causes of deaths, DALYs, and geriatric syndromes in Iranian elderly people between 1990 and 2010: findings from the global burden of disease study 2010. Arch Iran Med. 2015;18(8):462–79.Google Scholar
  23. Norton WH. Toward developmental models of psychiatric disorders in zebrafish. Front Neural Circuits. 2013;7:79.CrossRefGoogle Scholar
  24. Pei DS, Sun YH, Long Y, Zhu ZY. Inhibition of no tail (ntl) gene expression in zebrafish by external guide sequence (EGS) technique. Mol Biol Rep. 2008;35(2):139–43.CrossRefGoogle Scholar
  25. Pfeffer PL, Gerster T, Lun K, Brand M, Busslinger M. Characterization of three novel members of the zebrafish Pax2/5/8 family: dependency of Pax5 and Pax8 expression on the Pax2.1 (noi) function. Development. 1998;125(16):3063–74.Google Scholar
  26. Rahmani AH, Al Shabrmi FM, Allemailem KS, Aly SM, Khan MA. Implications of green tea and its constituents in the prevention of cancer via the modulation of cell signalling pathway. Biomed Res Int. 2015;2015:925640.Google Scholar
  27. Rottbauer W, Wessels G, Dahme T, Just S, Trano N, Hassel D, et al. Cardiac myosin light chain-2: a novel essential component of thick-myofilament assembly and contractility of the heart. Circ Res. 2006;99(3):323–31.CrossRefGoogle Scholar
  28. Sang S, Lambert JD, Ho C-T, Yang CS. The chemistry and biotransformation of tea constituents. Pharmacol Res. 2011;64(2):87–99.CrossRefGoogle Scholar
  29. Sarma DN, Barrett ML, Chavez ML, Gardiner P, Ko R, Mahady GB, et al. Safety of green tea extracts: a systematic review by the US pharmacopeia. Drug Saf. 2008;31(6):469–84.CrossRefGoogle Scholar
  30. Schmidt M, Schmitz HJ, Baumgart A, Guedon D, Netsch MI, Kreuter MH, et al. Toxicity of green tea extracts and their constituents in rat hepatocytes in primary culture. Food Chem Toxicol. 2005;43(2):307–14.CrossRefGoogle Scholar
  31. Segner H. Zebrafish (Danio rerio) as a model organism for investigating endocrine disruption. Comp Biochem Physiol C. 2009;149(2):187–95.Google Scholar
  32. Shao J, Chen D, Ye Q, Cui J, Li Y, Li L. Tissue regeneration after injury in adult zebrafish: the regenerative potential of the caudal fin. Dev Dyn. 2011;240(5):1271–7.CrossRefGoogle Scholar
  33. Sharangi AB. Medicinal and therapeutic potentialities of tea (Camellia sinensis L.)—a review. Food Res Int. 2009;42(5–6):529–35.CrossRefGoogle Scholar
  34. Stangl V, Lorenz M, Stangl K. The role of tea and tea flavonoids in cardiovascular health. Mol Nutr Food Res. 2006;50(2):218–28.CrossRefGoogle Scholar
  35. Tang FY, Chiang EP, Shih CJ. Green tea catechin inhibits ephrin-A1-mediated cell migration and angiogenesis of human umbilical vein endothelial cells. J Nutr Biochem. 2007;18(6):391–9.CrossRefGoogle Scholar
  36. Wang CC, Chu KO, Chong WS, Li WY, Pang CP, Shum AS, et al. Tea epigallocatechin-3-gallate increases 8-isoprostane level and induces caudal regression in developing rat embryos. Free Radic Biol Med. 2007;43(4):519–27.CrossRefGoogle Scholar
  37. Westerfield M. The zebrafish book: a guide for the laboratory use of zebrafish (Danio rerio): M. Westerfield 2007Google Scholar
  38. Wilkinson DG, Bhatt S, Chavrier P, Bravo R, Charnay P. Segment-specific expression of a zinc-finger gene in the developing nervous system of the mouse. Nature. 1989;337(6206):461–4.CrossRefGoogle Scholar
  39. Yang CS, Wang X, Lu G, Picinich SC. Cancer prevention by tea: animal studies, molecular mechanisms and human relevance. Nat Rev Cancer. 2009;9(6):429–39.CrossRefGoogle Scholar
  40. Yang CS, Li G, Yang Z, Guan F, Chen A, Ju J. Cancer prevention by tocopherols and tea polyphenols. Cancer Lett. 2013;334(1):79–85.CrossRefGoogle Scholar

Copyright information

© Springer Nature B.V. 2018

Authors and Affiliations

  1. 1.Tianjin Key Laboratory of Tumor Microenvironment and Neurovascular Regulation, Department of Histology and Embryology, School of MedicineNankai UniversityTianjinChina
  2. 2.Department of Pathogenic Biology, School of MedicineNankai UniversityTianjinChina
  3. 3.College of HorticultureNanjing Agricultural UniversityNanjingChina
  4. 4.Beijing Center for Physical and Chemical AnalysisBeijingChina

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