Studies on combination of oxaliplatin and dendrosomal nanocurcumin on proliferation, apoptosis induction, and long non-coding RNA expression in ovarian cancer cells
Drug resistance remains a major challenge in the treatment of patients with ovarian cancer. Therefore, the development of new anticancer drugs is a clinical priority to develop more effective therapies. New approaches to improve clinical outcomes and limit the toxicity of anticancer drugs focus on chemoprevention. The aim of this study was to determine the effects of dendrosomal nanocurcumin (DNC) and oxaliplatin (Oxa) and their combination on cell death and apoptosis induction in human ovarian carcinoma cell lines analyzed by MTT assay and flow cytometry, respectively. The synergism effect of Oxa and DNC was analyzed using the equation derived from Chou and Talalay. In addition, real-time PCR was used to measure the effect of this combination on the expression levels of long non-coding RNAs with different expression in ovarian cancer and normal ovaries. Our data showed that the effect of DNC on cell death is more than curcumin alone in the same concentration. The greatest cell death effect was observed in combination of Oxa with DNC, while Oxa was added first, followed by DNC at 4 h interval (0/4 h). The findings indicated that DNC induced apoptosis significantly in both cell lines as compared to control groups; however, combination of both agents had no significant effect in apoptosis induction. In addition, combination of both agents significantly affects the relative expression of long non-coding RNAs investigated in the study as compared with mono therapy.
KeywordsOvarian cancer Oxaliplatin Dendrosomal nanocurcumin Long non-coding RNA
Long non-coding RNA
Fourier transform infrared
Nuclear magnetic resonance
High-performance liquid chromatography with a diode-array detector
Dynamic light scattering
Fetal bovine serum
3-(4,5-Dimethylthiazole-yl)-2, 5-diphenyltetrazolium bromide
The half maximal inhibitory concentration of cells
The original research described in this paper is part of the Ph.D. thesis of ESH. The present work was supported financially by grant no. 94123 from Kashan University of Medical Sciences, Kashan, Iran, and grant no. 95819873 from Iran National Science Foundation. We also thank the Deputy of Research and Technology, Ministry of Health and Medical Education of Iran for research grant support.
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ESH and HN provided direction and guidance throughout the preparation of this manuscript. ESH and HN conducted the literature and drafted the manuscript. Other authors reviewed the manuscript and made significant revisions on the drafts. All authors read and approved the final version.
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The authors declare that they have no competing interests.
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