Cell Biology and Toxicology

, Volume 27, Issue 5, pp 333–342 | Cite as

Cytotoxicity, permeability, and inflammation of metal oxide nanoparticles in human cardiac microvascular endothelial cells

Cytotoxicity, permeability, and inflammation of metal oxide nanoparticles
  • Jing Sun
  • Shaochuang Wang
  • Dong Zhao
  • Fei Han Hun
  • Lei Weng
  • Hui LiuEmail author


Wide applications and extreme potential of metal oxide nanoparticles (NPs) increase occupational and public exposure and may yield extraordinary hazards for human health. Exposure to NPs has a risk for dysfunction of the vascular endothelial cells. The objective of this study was to assess the cytotoxicity of six metal oxide NPs to human cardiac microvascular endothelial cells (HCMECs) in vitro. Metal oxide NPs used in this study included zinc oxide (ZnO), iron(III) oxide (Fe2O3), iron(II,III) oxide (Fe3O4), magnesium oxide (MgO), aluminum oxide (Al2O3), and copper(II) oxide (CuO). The cell viability, membrane leakage of lactate dehydrogenase, intracellular reactive oxygen species, permeability of plasma membrane, and expression of inflammatory markers vascular cell adhesion molecule-1, intercellular adhesion molecule-1, macrophage cationic peptide-1, and interleukin-8 in HCMECs were assessed under controlled and exposed conditions (12–24 h and 0.001–100 μg/ml of exposure). The results indicated that Fe2O3, Fe3O4, and Al2O3 NPs did not have significant effects on cytotoxicity, permeability, and inflammation response in HCMECs at any of the concentrations tested. ZnO, CuO, and MgO NPs produced the cytotoxicity at the concentration-dependent and time-dependent manner, and elicited the permeability and inflammation response in HCMECs. These results demonstrated that cytotoxicity, permeability, and inflammation in vascular endothelial cells following exposure to metal oxide nanoparticles depended on particle composition, concentration, and exposure time.


Cytotoxicity Permeability Inflammation Metal oxide nanoparticles Vascular endothelial cells 





Iron(III) oxide


Yttrium oxide


Cerium oxide


Zinc oxide


Iron(II,III) oxide


Magnesium oxide


Aluminum oxide


Copper(II) oxide


Endothelial cell medium


Human cardiac microvascular endothelial cells


Lactate dehydrogenase


Reactive oxygen species


Vascular cell adhesion molecule-1


Intercellular adhesion molecule 1


Macrophage cationic peptide-1





This study was supported by grants from the Shanghai Municipal Health Bureau (2008Y077) and the Sub-Project of the National Grand Fundamental Research 863 Program of China (2007AA021802 and 2007AA022004).

Conflict of interest

The authors have no conflict of interest.


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Copyright information

© Springer Science+Business Media B.V. 2011

Authors and Affiliations

  • Jing Sun
    • 1
  • Shaochuang Wang
    • 2
  • Dong Zhao
    • 1
  • Fei Han Hun
    • 1
  • Lei Weng
    • 1
  • Hui Liu
    • 3
    Email author
  1. 1.Shanghai First Maternity and Infant HospitalTongji UniversityShanghaiChina
  2. 2.Department of Hepatobiliary Pancreatic SurgeryThe Huai’an First People’s Hospital Affiliated to Nanjin Medical UniversityJiangsuPeople’s Republic of China
  3. 3.Eastern Hepatobiliary Surgery HospitalSecond Military Medical UniversityShanghaiPeople’s Republic of China

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