Cell Biology and Toxicology

, Volume 26, Issue 6, pp 509–526

Antioxidant enzyme activity and lipid peroxidation in the blood of rats co-treated with vanadium (V+5) and chromium (Cr+3)

  • Agnieszka Ścibior
  • Halina Zaporowska
  • Agnieszka Wolińska
  • Jarosław Ostrowski
Article

DOI: 10.1007/s10565-010-9160-8

Cite this article as:
Ścibior, A., Zaporowska, H., Wolińska, A. et al. Cell Biol Toxicol (2010) 26: 509. doi:10.1007/s10565-010-9160-8

Abstract

Selected biochemical parameters were studied in the blood of outbred, male Wistar rats which daily received to drink deionized water (Group I, control) or solutions of: sodium metavanadate (SMV; 0.100 mg V/mL)—Group II; chromium chloride (CC; 0.004 mg Cr/mL)—Group III; and SMV-CC (0.100 mg V and 0.004 mg Cr/mL)—Group IV for a 12-week period. The diet and fluid intake, body weight gain, and food efficiency ratio (FER) diminished significantly in the rats of Groups II and IV, compared with Groups I and III. The plasma total antioxidant status (TAS) as well as the MDA and the l-ascorbic acid level in the erythrocytes (RBCs) remained unchanged in all the groups, whereas the plasma l-ascorbic acid concentration decreased markedly in Group II, compared with Group III. The activities of Cu,Zn-superoxide dismutase (Cu,Zn-SOD), catalase (CAT), cellular glutathione peroxidase (cGSH-Px), and glutathione reductase (GR) in RBCs remained unaltered in all the treated rats. However, the activity of glutathione S-transferase (GST) and the content of reduced glutathione (GSH) in RBCs decreased and increased, respectively, in Groups II, III, and IV, compared with Group I. A vanadium–chromium interaction which affected the GST activity was also found. To summarize, SMV and CC administered separately or in combination in drinking water for 12 weeks did not alter either lipid peroxidation (LPO) or the activities of Cu,Zn-SOD, CAT, cGSH-Px, and GR, which allows a conclusion that both metals in the doses ingested did not reveal their pro-oxidant potential on RBCs.

Keywords

Antioxidants Erythrocytes Lipid peroxidation Rats Trivalent chromium Total antioxidant status Vanadate 

Abbreviations

AAS

atomic absorption spectrometry

ABTS

2,2′-azino-bis-3-ethyl-benzothiazoline-6-sulfonic acid

ABTS•+

ABTS radical cation

AMV

ammonium metavanadate

BMOV

bis(maltolato)oxovanadium(IV)

CAT

catalase

CC

chromium chloride

CDNB

1-chloro-2,4-dinitrobenzene

cGSH-Px

cellular glutathione peroxidase

CNS

central nervous system

Cr

chromium

Cr2+

divalent chromium

Cr3+

trivalent chromium

Cr5+

pentavalent chromium

Cr6+

hexavalent chromium

CrCl3

chromium chloride

CrPic

chromium picolinate

Cu,Zn-SOD

Cu, Zn-superoxide dismutase

DMH

dimethylhydrazine

DNA

deoxyribonucleic acid

FER

food efficiency ratio

GF-AAS

graphite furnace atomic absorption spectrometry

GR

glutathione reductase

GSH

reduced glutathione

GSH-Px

glutathione peroxidase

GST

glutathione S-transferase

γ-GCS

gamma-glutamylcysteine synthetase

Hb

hemoglobin

HbA1C

glycated hemoglobin

H2O2

hydrogen peroxide

HO

hydroxyl radical

ICP-AES

inductively coupled plasma-atomic emission spectrometry

K2EDTA

bipotassium ethylene-diaminetetraacetic acid

LOOH

lipid hydroperoxides

LPO

lipid peroxidation

MDA

malondialdehyde

NaCl

sodium chloride

NADH

reduced nicotinamide adenine dinucleotide

NaVO3

sodium metavanadate

NH4VO3

ammonium metavanadate

NPY

neuropeptide Y

O2•−

superoxide radical

PBS

buffered solution of physiological salt

RBCs

red blood cells

ROS

reactive oxygen species

SH

sulfhydryl group

SMV

sodium metavanadate

SMV-CC

sodium metavanadate and chromium chloride

SOD

superoxide dismutase

TAS

total antioxidant status

TBARS

thiobarbituric acid reactive substances

V

vanadium

V5+

pentavalent vanadium

VO2+

vanadyl

Copyright information

© Springer Science+Business Media B.V. 2010

Authors and Affiliations

  • Agnieszka Ścibior
    • 1
  • Halina Zaporowska
    • 1
  • Agnieszka Wolińska
    • 2
  • Jarosław Ostrowski
    • 3
  1. 1.Department of Cell Biology, Institute of Environmental ProtectionJohn Paul II Catholic University of LublinLublinPoland
  2. 2.Department of Biochemistry and Environmental Chemistry, Institute of BiotechnologyCatholic University of LublinLublinPoland
  3. 3.Voivodship Inspectorate of Environmental ProtectionLublinPoland

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