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Cell Biology and Toxicology

, Volume 26, Issue 6, pp 499–507 | Cite as

A preliminary study: the anti-proliferation effect of salidroside on different human cancer cell lines

  • Xiaolan HuEmail author
  • Shuxin Lin
  • Daihua Yu
  • Shuifeng Qiu
  • Xianqi Zhang
  • Ruhuan Mei
Article

Abstract

Salidroside (p-hydroxyphenethyl-beta-d-glucoside), which is present in all species of the genus Rhodiola, has been reported to have a broad spectrum of pharmacological properties. The present study, for the first time, focused on evaluating the effects of the purified salidroside on the proliferation of various human cancer cell lines derived from different tissues, and further investigating its possible molecular mechanisms. Cell viability assay and [3H] thymidine incorporation were used to evaluate the cytotoxic effects of salidroside on cancer cell lines, and flow cytometry analyzed the change of cell cycle distribution induced by salidroside. Western immunoblotting further studied the expression changes of cyclins (cyclin D1 and cyclin B1), cyclin-dependent kinases (CDK4 and Cdc2), and cyclin-dependent kinase inhibitors (p21Cip1 and p27Kip1). The results showed that salidroside inhibited the growth of various human cancer cell lines in concentration- and time-dependent manners, and the sensitivity to salidroside was different in those cancer cell lines. Salidroside could cause G1-phase or G2-phase arrest in different cancer cell lines, meanwhile, salidroside resulted in a decrease of CDK4, cyclin D1, cyclin B1 and Cdc2, and upregulated the levels of p27Kip1 and p21Cip1. Taken together, salidroside could inhibit the growth of cancer cells by modulating CDK4-cyclin D1 pathway for G1-phase arrest and/or modulating the Cdc2-cyclin B1 pathway for G2-phase arrest.

Keywords

Salidroside Cancer cells Cell proliferation Cell cycle arrest 

Abbreviations

ATCC

American Tissue Culture Collection

CDK

Cyclin-dependent kinase

CDKI

Cyclin-dependent kinase inhibitor

CDK4

Cyclin-dependent kinase 4

Cdc2

Cell division cycle protein 2

Cip1

Cdk-interacting protein1

Kip1

Kinase inhibitor protein

MTT

3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyl tetrazolium bromide

PI

Propidium iodide

Notes

Acknowledgement

This study was partly supported by a grant from Medicine Health Science Research Fund of PLA (No: 01L003) and was supported in part by a grant awarded to Zhejiang Provincial Natural Science Foundation (Y2080271).

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Copyright information

© Springer Science+Business Media B.V. 2010

Authors and Affiliations

  • Xiaolan Hu
    • 1
    Email author
  • Shuxin Lin
    • 2
  • Daihua Yu
    • 2
  • Shuifeng Qiu
    • 1
  • Xianqi Zhang
    • 1
  • Ruhuan Mei
    • 1
  1. 1.Department of Pathology and PathophysiologyZhejiang University School of MedicineHangzhouChina
  2. 2.Department of PathophysiologyFourth Military Medical UniversityXi’anChina

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