Cell Biology and Toxicology

, Volume 26, Issue 4, pp 355–365 | Cite as

l-carnitine modulates blood platelet oxidative stress

  • Joanna Saluk-JuszczakEmail author
  • Beata Olas
  • Barbara Wachowicz
  • Rafal Glowacki
  • Edward Bald


The oxidative stress induced by acute exertion may interfere with blood platelet activation. The beneficial effect of l-carnitine (γ-trimethylamino-β-hydroxybutyric acid) on oxidative stress in blood platelets has not been fully investigated; however, different studies indicate that this compound modulates platelet functions. The aim of our study was to assess the effects of l-carnitine on platelet activation and oxidative/nitrative protein damage (determined by the levels of protein carbonyl groups, thiol groups, and 3-nitrotyrosine residues) in resting blood platelets or platelets treated with peroxynitrite (ONOO, a strong physiological oxidant) in vitro. We also investigated the effects of l-carnitine on the level of platelet glutathione and on the formation of superoxide anion radicals \( \left( {{\hbox{O}}_2^{ - \bullet }} \right) \), lipid peroxidation measured by thiobarbituric acid reactive substances (TBARS) in blood platelets stimulated by thrombin (a strong physiological agonist), and platelet aggregation induced by adenosine diphosphate (a strong physiological stimulator). We have observed that carnitine decreases platelet activation (measured by platelet aggregation, the generation of \( {\hbox{O}}_2^{ - \bullet } \), and TBARS production). Moreover, our results in vitro demonstrate that carnitine may protect against oxidation of thiol groups induced by ONOO. Thus, carnitine may have some protectory effects against oxidative changes induced in blood platelets.


Blood platelets Carnitine Oxidative stress Peroxynitrite 



This work was supported by grant 505/374 from the University of Lodz.


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Copyright information

© Springer Science+Business Media B.V. 2010

Authors and Affiliations

  • Joanna Saluk-Juszczak
    • 1
    Email author
  • Beata Olas
    • 1
  • Barbara Wachowicz
    • 1
  • Rafal Glowacki
    • 2
  • Edward Bald
    • 2
  1. 1.Department of General Biochemistry, Institute of BiochemistryUniversity of LodzLodzPoland
  2. 2.Department of Environmental ChemistryUniversity of LodzLodzPoland

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